فراوانی آنتی بادی ضد نئوسپورا کانینوم در نمونه‌های شیر تعدادی از گاوداری‌های شهرستان شهریار

نوع مقاله: مقاله کامل

نویسندگان

1 دانش آموخته کارشناسی ارشد رشته انگل شناسی پزشکی دانشگاه تربیت مدرس تهران ، تهران- ایران

2 گروه انگل شناسی دانشگاه تربیت مدرس تهران ، تهران- ایران

چکیده

نئوسپورا کانینوم انگل داخل سلولی اجباری از شاخه آپی‌کمپلکسا بوده و باعث سقط جنین در گاو و فلج عصبی عضلانی در سگ می‌گردد. با توجه به ضررهای اقتصادی وارده به صنعت دامپروری در اثر سقط جنین و کاهش تولید شیر ناشی از نئوسپورا کانینوم، فراوانی این تک یاخته در گاوهای شیری منطقه شهریار مورد ارزیابی قرار گرفت . با توجه به پراکندگی دامی 150 نمونه شیرخام از 5 دامداری گاو شیری از 4 منطقه شهریار جمع آوری و با روش الایزای غیر مستقیم از لحاظ وجود آنتی بادی ضد نئوسپورا کانینوم مورد ارزیابی قرار گرفتند. از مجموع 150 نمونه، 22% از لحاظ وجود آنتی بادی ضد نئوسپورا کانینوم مثبت برآورد گردید. با استفاده از آزمون مجذور کای بیشترین میزان شیوع در گاوهای بالاتر از 4 سال سن مشاهده گردید (۰۳۹/۰=P-value)آزمون الایزای غیر مستقیم بکار رفته در شیر بنظر می رسد مفید و مقرون به صرفه برای نظارت بر برنامه های استراتژی کنترل نئوسپورا کانینوم در مزارع پرورش گاو شیری باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Prevalence of antibodies to Neospora caninum in milk samples from dairy farms in the Shahriyar

نویسندگان [English]

  • Kamal Taheri Lak 1
  • Javid Sadraei 2
  • A. Dalimi 2
1 Taheri Lak, K., Department of Parasitology, Faculty of Medical Sciences Tarbiat Modares University, Tehran, Iran.
2 Department of Parasitology, Faculty of Medical Sciences Tarbiat Modares University, Tehran, Iran.
چکیده [English]

Neospora caninum is an obligate intracellular parasite in the Apicomplexa phylum. The parasite causes abortion in cattle and Neuromascular paralysis in dog. Given the economic losses incurred by abortion caused by Neospora caninum and reduced milk production, in this study the frequncy of Neospora was evaluated by ELISA in the Shahriyar area. 150 samples of raw milk of 5 dairy cattle herds collected and tested for the detection of antibodies against Neospora caninum by ELISA. 22% of the samples were positive for the presence of antibodies against Neospora. The highest prevalence was observed in cows older than 4 years of age (P-value =0.039). The indirect ELISA used in milk seems to be a useful for epidemiological surveys, and to increase the level of control strategy of Nespora caninum in dairy cattle farms.

کلیدواژه‌ها [English]

  • Neospora caninum
  • Milk
  • ELISA
  • Shahriyar
1. Catherine, S., T. Sohn Tim, L. Cheng Michael, D. Drummond Eric, J. Peng Sarah, J. Vermont Dong Xia Stephen, M. Cheng Jonathan and J. Peter. (2011) Identification of Novel Proteins in Neospora caninum Using an Organelle Purification and Monoclonal Antibody Approach. PLoS One. 6(4): e18383.
2. Chanlun, A., U. Emanuelson, S. Chanlun, S. Aiumlamai and C. Bjorkman. (2006) Application of repeated bulk milk testing for identification of infection dynamics of Neospora caninum in Thai dairy herds. Veterinary Parasitology. 136: 243–250.
3. Darwich, L., O. Cabezon, I. Echeverria, M. Pabon, I. Marco, R. Lopez, O. Alejos, F. Gatius, S. Lavin and S. Almeria. (2012) Presence of Toxoplasma gondii and Neospora caninum DNA in the brain of wild birds.Veterinary Parasitology. 183 (3-4): 377–381.
4. Dijkstra, T., H.W. Barkema, M. Eysker and W. Wouda. (2001) Evidence of post-natal transmission of Neospora caninum in Dutch dairy herds. International Journal for Parasitology. 31: 209–215.
5. Dubey, J.P. and D. S. Lindsay. (1996) A review of Neospora caninum and neosporosis. Veterinary Parasitology. 67: 1–59.
6. Dubey, J. P. (2005) Neosporosis in cattle. Vet. Clin. Of North America, Food animal practice. 21: 473-483.
7. Frossling, J., A. Uggla and C. Bjorkman. (2005) Prevalence and transmission of Neospora caninum within infected Swedish dairy herds. Veterinary Parasitology. 128: 209–218.
8. Frossling, J., A. dtvedt, A. Lindberg and C. rkman. (2008) Spatial analysis of Neospora caninum distributio cattle from Sweden. Geospatial Health . 3(1): 39-45.
9. Garcia, D. P., L. M. Regidor, E. Ortega, V. S. F. Collantes, M. A. de Oliveria and A. C. Da Silva. (2009) Isolation and biological characterization of a new isolate of Neospora caninum from an asymptomatic calf in Brazil. Acta Parasitologica. 54: 180-185.
10. Gharekhani, J., H. Heidari and H. Akbarein. (2012) Seroepidemiology of Neospora caninum in Iranian native and crossbreed cattle : A cross sectional study. Journal of Veterinary Research. 67(4): 325-329
11. Gonzalez, M., J. A. Castro, C. Carro and M. Mezo. (Aug 2011) anti-Neospora caninum antibodies in milk in relation to production losses in dairy cattle. Preventive Veterinary Medicine. 1;101(1-2):58-64. 1; 101(1-2): 58-64.
12. Hajikolaei, M.R., H. Hamidinejat and S. Goraninejad. (2008) Serological study of Neospora caninum infection in cattle from Ahvaz. Iranian International Journal of Veterinary Researches. 2:1:63-66.
13. Jensen, A.M., C. Bjorkman, A.M. Kjeldsen, A. Wedderkopp, C. Willadsen, A. Uggla and P. Lind. (1999) Associations of Neospora caninum seropositivity with gestation number and pregnancy outcome in Danish dairy herds. Preventive Veterinary Medicine. 40: 151-163.
14. King, J. S., J. Slapeta, D. J. Jenkins, S. E. Al-Qassab, J. T. Ellis and P. A. Windsor. (2010) Australian dingoes are definitive hosts of Neospora caninum. International Journal of Parasitology. 40 (8): 945–950.
15. McAllister, M. M., J. P. Dubey, D. S. Lindsay, W. R. Jolley, R. A. Wills and A. M. McGuire. (1998) Dogs are definitive hosts of Neospora caninum. International Journal for Parasitology. 28 (9): 1473–1478..
16. Moayer, F., A. Ataei, F. Moosakhani and A. Bahonar. (2011) Pathologic findings of aborted fetuses in industrial farms around Tehran. Journal of Veterinary Clinics. 2(3): 155-156.
17. Nematollahi, A., M. Jafari and G. Moghadam. (2011) Neospora caninum infection study using standard household ELISA in dairy farms Tabriz around. Iranian Veterinary Journal. 7(1): 62
18. Romero, D., Z. Garcia, F. Montiel, M. Dominguez and L. Medina Esparza. (2010) Seroprelence of Neospora caninum antibodies in cattle in Veracarz Mexico. Journal of Animal and Veterinary Advances. 9: 1445- 1451.
19. Sabevarinejad, Gh., A. Dalimi, F. Ghafarifar and M. Forouzandeh Moghadam. (2013) Serological survey of Neospora caninum infection in Holstein dairy cattle from Khoramabad region. Veterinary Journal (Pajouhesh & Sazandegi) 26(4): 45-48.
20. Sadrebazzaz, A., H. Haddadzadeh, K. Esmailnia, G. Habibi, M. Vojgani and R. Hashemifesharaki. (2004) Serological prevalence of Neospora caninum in healthy and aborted dairy cattle in Mashhad,Iran. Veterinary Parasitology. 124(3-4):201-204.
21. Sadrebazzaz, A., G. Habibi, H. Haddadzadeh and J.Ashrafi. (2007) Evaluation of bovine abortiob associated with Neospora caninum by differenet diagnostic techniques in Mashhad, Iran. Parasitology Researches.101:413-417
22. Thurmond , M. C. and J. P. Picanso. (2007) Asurveillance system of bovine abortion. Prev. Vet. Med. 8: 41-53.
23. Trees, A. J. and D. J. Williams. (2005) Endogenous and exogenous transplacental infection in Neospora caninum and Toxoplasma gondii. Trends Parasitol. 21: 558–561.