تعیین ویژگی‌های ژنتیکی سویه‌های مایکوباکتریوم ایویوم زیر گونه پاراتوبرکولوزیس III & V و 316F با استفاده از یک راهبرد چندگانه

نوع مقاله: مقاله کامل

نویسندگان

1 بخش واکسن‌های باکتریایی هوازی دامپزشکی، مؤسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی

2 بخش تحقیقات دامپزشکی مرکز تحقیقات کشاورزی و منابع طبیعی استان اصفهان، سازمان تحقیقات، آموزش و ترویج کشاورزی

3 رئیس آزمایشگاه تشخیص مؤسسه تحقیقات و اکسن و سرم سازی رازی

4 بخش تحقیق و تولید توبرکولین و مالئین، مؤسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی

چکیده

دو سویه غیر بومی (Mycobacterium avium subspecies paratuberculosis (MAP 316F و MAP III & V به مدت نیم قرن در موسسه رازی نگهداری و از آن‌ها برای تهیه پاراتوبرکولین استفاده شده است. به منظور شناسایی خصوصیات ژنتیکی این دو سویه یک استراتژی سه محور تعیین هویت (PCR-IS900, PCR-F57)، تعیین تیپ (PCR-Collins, PCR-gyrA, PCR-gyrB) و تعیین ژنوتیپ (MLVA-Thibault, SSR-Amonsin) به کار گرفته شد. در نتیجه اجرای این استراتژی هویت هر دو سویه به عنوان MAP تایید گردید و تیپ آنها از نوع گاوی شناخته شد. در ژنوتایپینگ تیپ MLVA آن‌ها INMV2 نشان داده شد که با نمونه فرانسوی (Merial) سویه MAP 316F همخوانی دارد. در تفسیر این یافته ها می‌توان گفت شباهت تیپ ژنتیکی دو سویه در SSR-Amonsin و MLVA-Thibault ممکن است اتفاقی باشد و یا نشانگر تعلق آنها به یک کلون اجدادی واحد باشد. علاوه بر این چون مؤسسه اتلیک ترکیه تامین کننده سویه MAP 316F مورد بررسی در این تحقیق می باشد، بنابراین احتمالا یک منبع فرانسوی تامین کننده این سویه برای مؤسسه اتلیک ترکیه بوده است. انتظار می رود اجرای این استراتژی بر روی جدایه‌های بومی ایران دانش مولکولار اپیدمیولوژی موجود را در رابطه با پاراتوبرکولوزیس در ایران افزایش دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Molecular identification of Mycobacterium avium subspecies paratuberculosis 316F and III & V strains by a multi-approach strategy

نویسندگان [English]

  • M. Mohrekesh Haghighat, 1
  • A.H. Shahmoradi 2
  • K. Tadayon 1
  • R. Keshavarz 3
  • R. Ghaderi 1
  • M. Sekhavati 1
  • Nader Mosavari 4
1 Tuberculin & Mallein Research and Production Department, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2 Veterinary Research Department, Isfahan Agriculture and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
3 Tuberculin & Mallein Research and Production Department, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
4 Tuberculin & Mallein Research and Production Department, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

For almost half a century Mycobacterium avium subspecies paratuberculosis (MAP) 316F and MAP III & V strains have been used in preparation of paratuberculin at Razi institute. In order to characterize their genomic properties, a multi-approach strategy was employed to authenticate their identity (PCR-IS900 or PCR-F57), their type (PCR-Collins, PCR-gyrA or PCR-gyrB) and their genotype (MLVA-Thibault or SSR-Amonsin). Consequently, both strains proved to be MAP and from a cattle type. In MLVA the genotype was found to be INMV2, a type identical to that of the French MAP 316F Merial sub-strain. In explanation of these observations, the identical patterns of both studies strains in MLVA as well as SSR typing might be a case of coincidence or a an indication of a mutual ancestral clone. Besides, the fact that the supplier of MAP 316F strain studied here is the Turkish Etlik institute, it is likely that the original source for this strain is French. Application of the strategy developed in this study on indigenous MAP isolates will improve the current molecular epidemiology understanding of paratuberculosis in Iran.

کلیدواژه‌ها [English]

  • IS900
  • F57
  • MLVA
  • SSR typing
  • gyrA
  • gyrB
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