مطالعه تجربی اثر متفورمین روی زخم سوختگی قرنیه در خرگوش

اصغری, احمد; هاشم زاده همایونی, سیده بهاره; مرتضوی, پژمان; جهاندیده, علیرضا

doi:10.22034/vj.2016.106438

مطالعه تجربی اثر متفورمین روی زخم سوختگی قرنیه در خرگوش

نوع مقاله : مقاله کامل

نویسندگان

¹ گروه جراحی دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

² دانش آموخته دکترای حرفه‌ای دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

³ گروه پاتوبیولوژی دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

10.22034/vj.2016.106438

چکیده

این تحقیق به منظور مطالعه تجربی اثر متفورمین بر روی ترمیم زخم سوختگی قرنیه در خرگوش انجام گرفت. در این تحقیق از 20 سر خرگوش نیوزلندی نر بالغ با میانگین وزنی 3-5/2 کیلوگرم سالم استفاده شد. بعد از بی‌هوشی عمومی، زخم قرنیه در چشم چپ تمام حیوانات ایجاد شد. بعد از برگشت از بی‌هوشی، خرگوش‌ها به 4 گروه 5 تایی تقسیم شدند. سپس در گروه اول متفورمین mg/kg 50 گاواژ شد، در گروه دوم متفورمین mg/kg 100، در گروه سوم متفورمین mg/kg 200 و در گروه چهارم آب مقطر تا 21 روز بعد از ایجاد زخم قرنیه روزانه گاواژ شد. 21 روز بعد از ایجاد سوختگی قرنیه، نمونه‌های بافتی از محل زخم اخذ و تحت بررسی آسیب‌شناسی بافتی قرار گرفتند. با توجه به مطالعات آسیب‌شناختی عروق‌زایی، میزان التهاب و ادم بستره قرنیه در خرگوش‌های تحت درمان با متفورمین در مقایسه با گروه شاهد بطور معنی‌داری کاهش اما لایه‌های بافت پوششی قرنیه افزایش یافته بود (05/0 >p). همچنین استفاده از متفورمین موجب بهبود نظم طبیعی رشته‌های کلاژن قرنیه شد (05/0 >p). براساس نتایج به دست آمده به نظر می‌رسد که متفورمین اثرات مناسب و قابل قبولی بر روند التیام بخشی و ترمیم زخم قرنیه خرگوش داشته باشد.

کلیدواژه‌ها

20.1001.1.24235407.1395.29.3.7.8

عنوان مقاله [English]

Experimental study of the effect of metformin on corneal burn ulcer healing in rabbit

نویسندگان [English]

A. Asghari ¹
B. Hashemzadeh Homaioni ²
P. Mortazavi ³
A. Jahandideh ¹

¹ Department of Veterinary Surgery, Science and Research Branch, Islamic Azad University, Tehran, Iran

² Veterinary Graduate Student, Science and Research Branch, Islamic Azad University, Tehran, Iran

³ Department of Veterinary Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran;

چکیده [English]

This study was conducted to examine the effect of metformin on corneal burn wound healing in rabbit. In this study, 20 adult healthy male New Zealand rabbits were used with an average weight of 2.5-3 kg. After general anesthesia, corneal ulcer in the left eye of the all animals was induced. After returning from anesthesia, the rabbits were randomly divided into 4 groups of 5 numbers. Then the first group was gavaged with 50 mg/kg of metformin, the second group with 100 mg/kg and the third group with 200 mg/kg while the fourth group was gavaged with water for 21 days. In the day 21 after making a corneal ulcer, tissue samples were taken and evaluated histopathologically. According to the pathological results, the vascularization, inflammation and edema of matrix of cornea significantly diminished while the epithelium of cornea increased in metformin-treated rabbits compared to control group (P<0.05). Also, administration of metformin significantly improved the natural structure of cornea collagen fibers (P<0.05). Based on the results, it seems metformin has favorable effect on the healing process in rabbit’s corneal ulcer.

کلیدواژه‌ها [English]

Corneal ulcer
Metformin
NaOH
Rabbit

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