بررسی حضور E.coli O157:H7 به روش PCR و ارزیابی مقاومت به آنتی بیوتیک های بتالاکتام در E. coliهای جداسازی شده از کبوترهای شهرکرد

نویسندگان

1 دانشجوی دانشکده دامپزشکی و عضو پژوهشکده بیماری های مشترک انسان و دام دانشگاه شهرکرد

2 دانشیار گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه آزاد اسلامی واحد شهرکرد

3 دانشجوی دانشکده دامپزشکی دانشگاه شهرکرد

4 دانشجوی دانشکده داروسازی، دانشگاه علوم پزشکی جندی شاپور اهواز

چکیده

کبوترها می توانند حامل بعضی از عوامل بیماری زای انسانی باشند و در انتقال و گسترش عوامل عفونی مقاوم به دارو به انسان نقش ایفا کنند. با توجه به اینکه بسیاری از مردم به نگه داری از کبوتر علاقه مندند، در این مطالعه به روش PCR به جستجوی E. coli  O157:H7 که عامل بیماری های روده ای انسانی و سندروم مرگبار اورمی همولیتیک در دنیا می باشد و همچنین ارزیابی مقاومت به آنتی بیوتیک های بتالاکتام در اشرشیا کلی های جداسازی شده از کبوترهای شهرکرد پرداخته شد. مجموعاً 180 نمونه مدفوعی از کبوتر از نقاط مختلف شهرکرد به وسیله ی سواب استریل جمع آوری گردید. سواب ها مستقیماً درون محیط آبگوشت تریپتون سوی (TSB) قرار داده شدند. در آزمایشگاه نمونه ها بر روی محیط های مکانکی آگار و مکانکی آگار سوربیتول دار حاوی مکمل سفکسیم و تلئوریت به عنوان محیط های انتخابی کشت داده شدند. سپس آزمون آنتی بیوگرام با استفاده از روش انتشار دیسک انجام شد. کلنی های مشکوک به E. coli O157:H7 به وسیله آزمون واکنش زنجیره ای پلی مراز (PCR) مورد آزمون قرار گرفتند. E. coli از 93 نمونه از 180 نمونه (7/51 درصد) جداسازی شد. میزان مقاومت جدایه ها به آنتی بیوتیک های سفوتاکسیم، ایمیپنم، سفیکسیم، آموکسی سیلین، سفالکسین، پنی سیلین جی و اگزاسیلین به ترتیب 1/1، 2/3، 6/8، 6/22، 9/40، 3/90 و 7/96 درصد بود. در هیچ کدام از نمونه ها E. coli  O157:H7 یافت نگردید. اگرچه کبوترها در این منطقه منبع یا حامل E. coli  O157:H7 نبودند، اما E. coli های مقاوم به آنتی بیوتیک های بتالاکتام را در خود جای داده بودند و می توانند عامل مهمی در انتقال عفونت های مقاوم به دارو از کبوتر به انسان، خصوصاً کودکان تلقی شوند و خطری جدی برای سلامت انسان ایجاد نمایند. با توجه به آنچه ذکر گردید توصیه می شود که صاحبان کبوتر و همچنین عموم مردم را از خطرات بالقوه ی نگه داری از کبوتر آگاه ساخت.

عنوان مقاله [English]

Detection of Escherichia coli O157:H7 by PCR and evaluation of resistance to beta-lactam antibiotics in Escherichia coli isolated from pigeons in Shahrekord, Iran

نویسندگان [English]

  • H Tahmasby 1
  • H Momtaz 2
  • M Rafiee Dolatabadi 3
  • S Barati 3
  • S Mehrabiyan 3
  • M Jafari 3
  • M Khosravi Farsani 3
  • M Ghasemi 3
  • S.V Ahmadi Salianeh 4
1 Student, Faculty of Veterinary Medicine and Member of Research Institute of Zoonotic Diseases, University of Shahrekord, Shahrekord, Iran
2 Associate Professor, Department of Microbiology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3 Student, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
4 Student, Faculty of Pharmacy, Ahvaz Jondishapour University of Medical Sciences, Ahvaz, Iran.
چکیده [English]

Pigeons can be carriers of some human pathogens and contribute to transmission and spread of drug resistant infectious agents to human. Considering many people’s interests to keep pigeons, present study was conducted to PCR detection of Escherichia coli O157:H7 that is responsible for outbreaks of human intestinal diseases and fatal haemolytic-uraemic syndrome worldwide and evaluation of resistance to beta-lactam antibiotics in Escherichia coli isolated from pigeons in Shahrekord. Altogether 180 samples of pigeon faeces were collected with sterile cotton swabs from different areas of Shahrekord, Iran. Swabs were placed directly into Tryptone Soya Broth (TSB). In the laboratory samples were streaked onto MacConkey agar and Cefixime Tellurite Sorbitol MacConkey agar as selective plating media. Antibiogram tests were then performed using disc diffusion method. Suspected colonies to E. coli O157:H7 were tested by polymerase chain reaction (PCR). E. coli was isolated from 93 (51.7%) of 180 the samples. Resistance of isolates to Cefotaxime, Imipenem, Cefixime, Amoxicillin, Cefalexin, Penicillin G and Oxacillin was 1.1%, 3.2% ,8.6% ,22.6% ,40.9% ,90.3% and 96.7% respectively. E. coli O157:H7 was not found in any samples. Although pigeons were not sources or carriers of E. coli O157:H7 in the region, they harbored beta-lactam antibiotics resistant E. coli and could be an important component of drugresistant infections transmission from pigeons to human, especially kids and can pose a risk to human health. Considering of what mentioned it is recommended to make pigeons owners and general public aware of potential dangers of pigeons keeping.

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