Effect of curcumin-loaded dendrimer nanoparticles on Giardia lamblia invivo

Document Type : Full Research Paper

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Babol Branch, Babol, Iran.

2 Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.

Abstract

Giardia lamblia is one of the most common intestinal parasites in Iran, which is treated with metronidazole. This drug hasGiardia lamblia is one of the most prevalent intestinal parasites in Iran, and its treatment typically involves the use of metronidazole. This drug has been associated with  side effects and there are numerous reports of parasite resistance to this drug. In light of the significance of giardiasis and the necessity for an alternative drug, the present study was undertaken to assess the  impact of the curcumin-loaded dendrimer nanoparticles on Giardia lamblia in vivo. In this experimental study, after preparation of the nanodendrimer-curcumin, size and chemical bonding between nanoparticles were determined by dynamic light scattering (DLS) and Fourier-transform infrared (FTIR) technique, respectively, following the preparation of the nanodendrimer-curcumin. The anti-giardial activity of the synthesized nanoparticle was then evaluated in infected mice by microscopic method. The final data were subjected to one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparisons test. The synthesized nanoparticles with an average size of 32 nm were found to be suitable for use indrug delivery systems. The results demonstrated that the highest efficacy of synthesized  nanoparticles in this study was observed at a dose of 16 mg/kg, with an efficacy of 79% in infected mice, which was statistically significant in comparison  to the negative control group (P<0.05). Given the superior efficacy of the synthesized nano dendrimer-curcumin against Giardia lamblia in mice compared to metronidazole, it can be proposed as a novel  candidate for the treatment of giardiasis. however ,further research is necessary to substantiate this claim.

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History

  • Receive Date: 01 June 2024
  • Revise Date: 29 August 2024
  • Accept Date: 31 August 2024

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