Document Type : Full Research Paper
Authors
1
Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute (Hesarak), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2
Department of Poultry disease diagnosis and research, Razi Vaccine and Serum Research Institute (Hesarak), Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
3
Associate Professor,Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
4
Department of Human vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
5
Department of Immunology, Pasteur Institute of Iran
Abstract
Researchers record new isolates of circulating influenza viruses. To prove the novelty of the virus phenotype, it is checked against the standard antiserum with a specific HI test. If the standard serum fails to completely neutralize the isolate, the genotypic change has increased to such an extent that it has caused a phenotypic change. In a research, the method of investigation and phenotypic identification of new strains of influenza (H9N2) in Iran was carried out according to the instructions of the CDC center. Three isolates of avian influenza virus (strain (H9N2) named A, B and C and the vaccine strain virus were passaged in SPF embryonated eggs for renewal. (1377) with fresh antigens and polyclonal serum, a special hemagglutination inhibition test was performed and the neutralization rate of viruses against polyclonal antiserum was evaluated .Of the three selected isolates, only sample C showed a difference of five wells compared to the vaccinal virus. The results of isolates A and B were similar to influenza vaccine virus. To ensure the accuracy of the results, the experiment was repeated three times, and the results of all three repetitions were similar. The results of this research proved the applicability of the special hemagglutination (HI) test method for the phenotypic identification of new isolates under the H9N2 type. It is recommended that the country's avian influenza reference laboratories use this method.
Keywords
Main Subjects
)