Serological and molecular study of Avibacterium paragallinarum infection in broilers at slaughter age in Karaj county

Document Type : Full Research Paper

Authors

1 Razi serum vaccine research institute

2 Department of Research and Diagnosis of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ministry of Agriculture Jihad, Karaj

3 undergraduate student of veterinary School of Islamic Azad University, Garmsar branch

Abstract

Avian Infectious Coryza (IC) is an acute respiratory disease in chickens caused by Avibacterium Paragallinarum (AP) which, results in a notable reduction in egg production in layers and performance in broiler chickens. IC exacerbates the severity of the condition when it occurs concurrently with other bacterial or  viral pathogens. There is a scarcity of data on the  prevalence of IC in broiler flocks in Karaj district. This study was initiallyconducted to determine the role of IC in broiler chickens through the use of  Agar Gel Precipitation (AGP), Serum Plate Agglutination (SPA), and species-specific PCR of HPG-2. A total of 30 thirty broiler flocks were sampled  for  blood and swabbing from choanal cleft (7-5 samples from each herd)  between February  and May 2022, at an avian slaughterhouse. The AGP test yielded  no serological results, whereas the SPA test indicated  a positive reaction in two flocks.  Molecular detection  via by PCR demonstrated the presence ofIC infection in 16 farms, representing a prevalence of 53%. The findings of this indicate that broiler chickens are  significantly exposed to IC,which poses a considerable threat to the viability of nearby commercial layer farms. It is therefore imperative that greater attention be paid to IC infection in epidemiological studies and  that effective solutions be provided  to deal with it in the country.

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History

  • Receive Date: 21 January 2024
  • Revise Date: 02 June 2024
  • Accept Date: 07 June 2024

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