Serological and molecular study of Avibacterium paragallinarum infection in broilers at slaughter age in Karaj District, Iran

Document Type : Full Research Paper

Authors

1 Razi serum vaccine research institute

2 Department of Research and Diagnosis of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ministry of Agriculture Jihad, Karaj

3 undergraduate student of veterinary School of Islamic Azad University, Garmsar branch

10.22092/vj.2024.364736.2120

Abstract

Avian Infectious Coryza (IC) is an acute respiratory disease in chicken caused by Avibacterium Paragallinarum (AP) which impose significant decline in egg production in layers and performance in broiler chicken. IC additionally aggravating severity of condition in coincident infection by other bacterial or virus pathogens. In the country, there is scarce information on the incidence of IC in broiler flocks in Karaj district. This study, as preliminary conducted to determine IC involvement in broilers chickens using by Agar Gel Precipitation (AGP), Serum Plate Agglutination (SPA) and species-specific PCR of HPG-2. Thirty broiler flocks were subjected for sampling of blood and swabbing from choanal cleft (7-5 samples from each herd) from February through May 2022, at avian slaughterhouse. We obtained serologically no result by AGP test but the SPA test in two herds revealed positive reaction. Molecular detection using by PCR reaction shown IC infection in 16 farms (53 %). This study showed that broiler chicken are heavily exposed to IC and has threatening role for nearby commercial layer farms. Therefore, it is necessary to pay more attention to IC infection in epidemiological studies and provide effective solutions to deal with it in the country.

Keywords

Main Subjects

Veterinary Research & Biological Products

Articles in Press, Accepted Manuscript
Available Online from 11 June 2024

Files

History

  • Receive Date: 21 January 2024
  • Revise Date: 02 June 2024
  • Accept Date: 07 June 2024

Share

How to cite

Statistics