Construction of recombinant protein Influenza A Virus Neuraminidase Gene expression in Baculovirus

Document Type : Full Research Paper

Authors

1 member of scientific staff

2 Associate Professor,Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

3 Department of Research and Production of antiserum , Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Two structural antigens, haemagglutinin (HA) and neuraminidase (NA), are attractive candidates for the development of a genetically engineered vaccine against influenza. Recombinant vaccines are produced by a simple and effective method, although expected to induce an immune response to a specific antigen, remain to be further improved for their high effectiveness. On the other hand, a potent and effective vaccine against influenza should be able to induce both humoral and cellular immune responses. In the present study, the NA gene, which is more stable than the HA one, was amplified by polymerase chain reaction (PCR), and the band appeared in the range of 1400 bp. Then cloned into a eukaryotic expression vector pFastBac HTA. The purity of the expressed NA protein was analyzed on SDS-PAGE electrophoresis. In the western blot, a band with a weight of about 41 kDa produced by the recombinant baculovirus containing NA is observed compared to the uninfected cells used as a negative control, and a recombinant neuraminidase is confirmed. Finally, neuraminidase (NA) gene clone can be used to make recombinant human influenza vaccine.

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Veterinary Research & Biological Products
Volume 37, Issue 3
October 2024
Pages 42-49

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History

  • Receive Date: 24 October 2023
  • Revise Date: 06 February 2024
  • Accept Date: 10 February 2024

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