Effect of Docosahexaenoic acid (DHA) on sperm quality during liquid storage of canine semen at 5 °C

Document Type : Full Research Paper

Authors

Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

Abstract

The storage process significantly affects the quality of dog semen because the dog's semen membrane is very sensitive to this process. In addition, this process leads to the production of free radicals and, as a result, to the peroxidation of the sperm membrane. The aim of the present study was to minimize the harmful effects of free oxygen radicals by adding different concentrations of docosahexaenoic acid to dog semen at 5 °C for five days. In this study, 25 ejaculates were collected three mixed-breed dogs and diluted in a Tris-based extender. Then they were divided into 5 parts into the control groups (without antioxidants) and amounts of 1.25, 2.5, 5 and 10 ng/mL docosahexaenoic acid in the extender. The evaluations (motility, morphology, DNA integrity, viability and total antioxidant capacity) were carried out after 0, 24, 48, 72 and 120 hours during storage at cool temperatures using CASA software and a light microscope for sperm parameters and using the ELISA method for the total amount of antioxidant capacity performed. The results showed that the groups containing docosahexaenoic acid had a significant increase in the percentage of cell viability, survival and total antioxidant capacity, as well as a significant increase in DNA integrity at 24, 48, 72 and 120 hours compared to the control group groups compared to the control group. became. The proportion of sperm with normal morphology increased significantly after 120 hours compared to the control group.

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History

  • Receive Date: 15 October 2023
  • Revise Date: 25 December 2023
  • Accept Date: 26 December 2023

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