Molecular detection of gyrB and oprL genes in Pseudomonas aeruginosa isolated from bovine mastitis in Maragheh city (East Azarbaijan province, Iran) in 2022.

Document Type : Full Research Paper

Authors

1 دانشگاه آزاد اسلامی واحد مراغه

2 Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran

Abstract

Pseudomonas aeruginosa is an important opportunistic pathogen that causes subclinical mastitis resistanted to treatment in lactating dairy cows. The aim of this research was to study the frequency of gyrB and oprL genes in Pseudomonas aeruginosa isolated from bovine mastitis in Maragheh city in 2022. In this descriptive cross-sectional study, 60 samples of Pseudomonas aeruginosa isolated from bovine mastitis which were identified by biochemical and staining tests. The samples were analyzed for the presence of the genes by PCR. Based on the results, the frequency of gyrB and oprL genes were observed in 10 (16.7%) and 3 (5%) isolates, respectively. Also, 3 isolates (5%) harbored both gyrB and oprL genes simultaneously. According to the results of this study, control of infection sources in mastitis caused by Pseudomonas aeruginosa is emphasized in order to control and prevent transmission of this bacterium.

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History

  • Receive Date: 26 July 2023
  • Revise Date: 20 November 2023
  • Accept Date: 21 November 2023

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