Synergistic effects of cholecystokinin isoforms (CCK4 and CCK8s) with glutamate on food intake in laying chickens

Document Type : Full Research Paper

Authors

1 Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2 university of tehran

3 Islamic Azad University, Tehran, Iran. Veterinary Medicine

Abstract

The present study was conducted with the aim of investigating the synergistic effects of CCK and glutamate on feeding behavior in layers. For this purpose. In the first experiment, the control solution, CCK4 (1 nmol), glutamate (75 nmol), and CCK4 + glutamate were injected intracerebroventricularly (ICV). The second experiment was performed according to the first experiment, with the difference that glutamate was injected with an effective dose (300 nmol). In the third experiment, the control solution, CCK8s (0.125 nmol), glutamate (75 nmol), and CCK8s + glutamate were administered. The fourth experiment was also the same as the third experiment and only the doses of CCK8s (1 nmol) and glutamate (300 nmol) were changed. According to the obtained results, the administration of doses under the effect of CCK4 and glutamate separately or simultaneously did not have a significant effect on the food intake of chickens (p<0.05). Injection of an effective dose of glutamate led to a significant decrease in feed intake, and simultaneous administration of CCK4 with glutamate did not change this hypophagic effect (p<0.05). Injection of sub-effective doses of CCK8s and glutamate had no significant effect on food intake (p<0.05), however, their simultaneous administration weakened food intake (p<0.05). Injection of effective doses of CCK8s and glutamate separately caused a significant decrease in food intake in chickens, and the administration of both of them strengthened this hypophagic effect (p<0.05). Finally, it seems that there is a synergistic effect between CCK8s and glutamate in the regulation of food intake in laying chickens.

Keywords

Main Subjects

References

  1. Atoji, Y., and M. R. Islam. 2009. Distribution of glutamate transporter 1 mRNA in the central nervous system of the pigeon (Columba livia). J Chem Neuroanat, 37(4), 234-244.
  2. Baghbanzadeh, A., and V. Babapour. 2007. Glutamate ionotropic and metabotropic receptors affect feed intake in broiler cockerels. Iran J Vet Med, 1(1), 125-129.
  3. Baldwin, B. A., T. R. Cooper, and R. F. Parrot. 1982. Effect of cholecystokinin octapeptide on food intake in pigs, Proc Nutr Soc, 41, 119.
  4. Campos, A., I. D. Port, and A. Acosta. 2022. Integrative Hedonic and Homeostatic Food Intake Regulation by the Central Nervous System: Insights from Neuroimaging. Brain Sci, 12(4), 431.
  5. Cawthon, C. R., and B. Claire. 2021. The critical role of CCK in the regulation of food intake and diet-induced obesity.Peptides, 138, 170492.
  6. Coyle, J. T., and P. Puttfarcken. 1993. Oxidative stress, glutamate, and neurodegenerative disorders. Science (New York, N.Y.), 262(5134), 689–695.
  7. Danbolt, N. C. 2001. Glutamate uptake. Progress in neurobiology, 65(1), 1–105.
  8. Deng, P. Y., Z. Xiao, A. Jha, D. Ramonet, T. Matsui, M. Leitges, H. S. Shin, J. E. Porter, J. D. Geiger, and S. Lei. 2010. Cholecystokinin facilitates glutamate release by increasing the number of readily releasable vesicles and releasing probability.J Neurosci, 30(15), 5136-48.
  9. Drewe, J., A. Gadient, L.C. Rovati, and C. Beglinger. 1992. Role of circulating cholecystokinin in control of fat-induced inhibition of food intake in humans, Gastroenterology, 102(5), 1654–1659.
  10. Gibbs, J., R. C. Young, and G. P. Smith. 1973. Cholecystokinin decreases food intake in rats, J Comp Physiol Psychol, 84(3), 488–495.
  11. Hermansen K. (1984). Effects of cholecystokinin (CCK)-4, nonsulfated CCK-8, and sulfated CCK-8 on pancreatic somatostatin, insulin, and glucagon secretion in the dog: studies in vitro. Endocrinology, 114(5), 1770–1775.
  12. Jelokhani, M., B. Vazir, M. Zendehdel, and A. Jahandideh. 2022. Interactions of Cholecystokinin and Glutamatergic Systems in Feeding Behavior of Neonatal Chickens. Arch Razi Inst, 77(2), 681-688.
  13. Kitagawa, K., H. Adachi, Y. Sekigawa, T. Yagami, S. Futaki, Y. J. Gu, and K. Inoue. 2004. Total chemical synthesis of large CCK isoforms using a thioester segment condensation approach. Tetrahedron, 60(4), 907-918.
  14. Maniscalco, J. W., C. M. Edwards, and L. Rinaman. 2020. Ghrelin signaling contributes to fasting-induced attenuation of hindbrain neural activation and hypophagic responses to systemic cholecystokinin in rats. Am J Physiol Regul Integr Comp Physiol, 318(5), R1014-23.
  15. Maslami, V., Y. S. Nur, and Y. Marlida. 2019. Effect of glutamate supplementation as a feed additive on performance of broiler chickens. J World's Poult Res, 9(3), 154-159.
  16. Miller, G. D. 2017. Appetite Regulation: Hormones, Peptides, and Neurotransmitters and Their Role in Obesity. Am J Lifestyle Med, 13(6), 586-601.
  17. Minaya, D. M., R. W. Larson, P. Podlasz, and K. Czaja. 2018. Glutamate-dependent regulation of food intake is altered with age through changes in NMDA receptor phenotypes on vagal afferent neurons.Physiol Behav, 189, 26-31.
  18. Mobarhan Fard, M., B. Vazir, M. Zendehdel, and A. Asghari. 2021. Interaction of Central Glutamatergic and Histaminergic Systems on Food Intake Regulation in Layer Chickens. Arch Razi Inst, 76(3), 537–551.
  19. Moran, T. H., P. J. Ameglio, H. J. Peyton, G. J. Schwartz, and P. R. McHugh. 1993. Blockade of type A, but not type B, CCK receptors postpones satiety in rhesus monkeys, Am J Physiol, 265(3), R620–R624.
  20. Mutluay, S. U., and H. Karataş. 2022. A Review of Glutamate and Its Receptors: Their Roles in Brain Physiology and Pathology. Acta Medica, 53(2), 99-109.
  21. Qi, W., D. Ding, and R. J. Salvi. 2008. Cytotoxic effects of dimethyl sulphoxide (DMSO) on cochlear organotypic cultures. Hear res, 236(1-2), 52–60.
  22. Rahmani, B., K. Mahdavi, M. Zendedel kheybari, M. Khodadadi, M. Keshavarz, M. Shahabi, and A. Baghbanzadeh. 2022. Role of central opioid receptors on serotonin-Induced hypophagia in the neonatal broilers. Iran J Vet Sci Tech, 14(1), 9-19.
  23. Rehfeld, J. F. 2020. Measurement of cholecystokinin in plasma with reference to nutrition related obesity studies. Nutr Res, 76, 1-8.
  24. Rotzinger, S., and F. J. Vaccarino. 2003. Cholecystokinin receptor subtypes: role in the modulation of anxiety-related and reward-related behaviours in animal models: 2001 CCNP Heinz Lehmann Award Paper. J Psychiatry Neurosci, 28(3), 171-181.
  25. Saito, E. S., H. Kaiya, T. Tachibana, S. Tomonaga, D. M. Denbow, K. Kangawa, and M. Furuse. 2005. Inhibitory effect of ghrelin on food intake is mediated by the corticotropin-releasing factor system in neonatal chicks. Regul pept, 125, 201-208.
  26. Schwartz, M. W., S. C. Woods, D. Porte Jr, R. J. Seeley, and D. G. Baskin. 2000. Central nervous system control of food intake. Nature, 404(6778), 661-671.
  27. Şener, K., E. N. Alver, and S. C. Cevher. 2022. An Overview of Appetite Regulation Mechanisms. Kocaeli j sci eng, 5(2), 178-193.
  28. Sutton, G. M., L. M. Patterson, and H. R. Berthoud. 2004. Extracellular signal-regulated kinase 1/2 signaling pathway in solitary nucleus mediates cholecystokinin-induced suppression of food intake in rats.J Neurosci, 24(45), 10240-7.
  29. Taati, M., H. Nayebzadeh, and M. Zendehdel. 2011. The effects of DL-AP5 and glutamate on ghrelin-induced feeding behavior in 3-h food-deprived broiler cockerels. J Physiol Biochem, 67, 217-223.
  30. Tachibana, T., K. Matsuda, M. Kawamura, H. Ueda, M. S. Khan, and M. A. Cline. 2012. Feeding-suppressive mechanism of sulfated cholecystokinin (26–33) in chicks. Comp Biochem Physiol Mol Integr Physiol, 161(4), 372-8.
  31. Van Tienhoven, A. T., and L. Juhasz. 1962. The chicken telencephalon, diencephalon and mesencephalon in stereotaxic coordinates. J Comp Neurol, 118, 185-197.
  32. Wank, S. A. 1998. I. CCK receptors: an exemplary family. Am J Physiol - Gastrointest. Liver Physiol, 274(4), G607-G613.
  33. Willard, S. S., and S. Koochekpour. 2013. Glutamate, glutamate receptors, and downstream signaling pathways. Int J Biol Sci, 9(9), 948.
  34. Wu, X., J. Y. Li, A. Lee, Y. X. Lu, S. Y. Zhou, and C. Owyang. 2020. Satiety induced by bile acids is mediated via vagal afferent pathways.JCI Insight, 5(14).
  35. Zeni, L. A., H. B. Seidler, N. A. De Carvalho, C. G.  Freitas, J. Marino-Neto, and M. A. Paschoalini. 2000. Glutamatergic control of food intake in pigeons: effects of central injections of glutamate, NMDA, and AMPA receptor agonists and antagonists. Pharmacol Biochem Behav, 65(1), 67-74.
Veterinary Research & Biological Products
Volume 37, Issue 1
March 2024
Pages 42-49

Files

History

  • Receive Date: 23 August 2023
  • Revise Date: 08 September 2023
  • Accept Date: 08 September 2023

Share

How to cite

Statistics