Phenotypic and genotypic characterization and determination of Listeria monocytogenes antibiotic resistance profile isolated from raw milk samples, worker's hands and milking equipment in Shahrekord

Document Type : Full Research Paper

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

4 Department of Pathobiology, Faculty of Veterinary Medicine, Bo Ali Sina University, Hamadan, Iran

5 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

Abstract

 contaminated ,milk raw as such food contaminated from humans to transmitted is ,listeriosis of cause the ,monocytogenes Listeria
 ,samples milk raw the in monocytogenes Listeria identify to conducted was research This .equipment milking contaminated or ,hands
 worker 40 and equipment milking 40 ,samples milk raw 70 ,study this In .workers dairy Shahrekord of hands and equipment milking
 Prs primers with PCR by amplified and boiling by extracted was colonies suspected the of DNA the ,confirmation biochemical ingFollow .agar Palcom on grown were they ,broth nutritional Brucella with enrichment initial an after and collected were samples hand
 the by assessed was isolates confirmed of resistance Antibiotic .)species monocytogenes Listeria (74DG69-DG and) genus Listeria(
 the on Based .assays biochemical using confirmed were) 9.33% (14 ,samples 150 Of .antibiotics five against method diffusion disc
 (0.67%) workers of sample hand one and) 0.67% (equipment milking of sample one ,)6.67% (milk raw of samples ten ,results PCR
 sample hand one and) 0.67% (equipment milking of sample one ,)5.33% (milk raw of samples eight and Listeria as identified were
 to resistance highest the exhibited isolates Confirmed .species monocytogenes Listeria for results positive gave) 0.67% (workers of
 Listeria ,Therefore .)80% (ampicillin and) 90% (amikacin to susceptibility highest the and) 60% (erythromycin and) 90% (ceftizoxime
 listeriosis of risks possible and versa vice and milk to hands' workers and equipment milking from transferred be can monocytogenes
.consumers milk raw threaten

Keywords

Main Subjects

References

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Veterinary Research & Biological Products
Volume 37, Issue 1
March 2024
Pages 34-41

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History

  • Receive Date: 13 March 2023
  • Revise Date: 06 July 2023
  • Accept Date: 06 July 2023

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