Epidemiologic and histopathological investigation of inclusion body hepatitis (IBH) in liver lesions of boiler chickens in Sanandaj slaughterhouse

Document Type : Short Communication

Authors

1 Graduate, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.

2 (Corresponding Author) Department of Pathobiology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

3 Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Kurdistan, Iran

4 Department of Animal Sciences, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran

Abstract

Adenoviruses belonging to group I in poultry (FAdV) cause essential diseases and are divided into five types from A to E. The strains of types D and E can cause severe damage to the liver tissue and cause a condition known as inclusion body hepatic (IBH). In recent years, outbreaks of IBH disease have been reported in different geographic regions, emphasizing the spread of the disease worldwide. The outbreak of IBH causes severe economic consequences, including poor performance and increased herd mortality. In this study, livers with lesions were sampled at two time intervals and transferred to the pathology laboratory for histopathological tests to investigate contamination in broiler poultry in poultry farms around Sanandaj city. Tissue samples were fixed in Buen's fixative solution. Sections were prepared from fixed tissues and stained with hematoxylin-eosin dye. Our research showed that according to the observation of eosinophilic inclusions inside the nucleus (as a pathognomic sign), the outbreak of IBH in broiler flocks slaughtered was 54.66%. The present study determined the IBH disease by examining the microscopic lesions in the liver of several killed chickens in the Sanandaj slaughterhouse.

Keywords

References

1-Gharbiani, M. Hatfinejad, K. and Shaik, J. (2018). Identification of the virus causing hepatitis with inclusion disease (IBH) by PCR method in broiler chickens of Isfahan province. Medical Microbiology Journal. 38:138-131.
 2-Moghadam, H. Pak inter-theoretic right, e. Hosseini, S. M. and Faryabi, S. (2017). Report of the occurrence of IBH in the beef herd of Gorgan city. Veterinary Laboratory Research, 10 (Special Letter No. 2 of the 12th Iranian Veterinary Congress), 155.‎
3- Alvarado, I. R., P. Villegas, J. El-Attrache, E. Jensen, G. Rosales, F. Perozo and L. B, Purvis. 2007. Genetic characterization, pathogenicity, and protection studies with an avian adenovirus isolate associated with inclusion body hepatitis. Avian diseases 51 (1): 27-32. 
4- Choi, K. S., S. J. Kye, J. Y. Kim, W. J. Jeon, E. K. Lee, K. Y. Park and H. W. Sung. 2012. Epidemiological investigation of outbreaks of fowl adenovirus infection in commercial chickens in Korea. Poultry Science 91: 2502-2506.
5- Dar, A., S. Gomis, I. Shirley, G. Mutwiri, R. Brownlie, A. Poter, V. Gerdts and S. K. Tikoo. 2012. Pathotypic and molecular characterization of a fowl adenovirus associated with inclusion body hepatitis in Saskatchewan chickens. Avian diseases 56: 73-81
6- Fadly, A. M., R. Winterfield and H. J. Olander. 1976. Role of the bursa of Fabricius in the pathogenicity of inclusion body hepatitis and infectious bursal disease viruses. Avian Diseases 20: 467-477.
7- Fitzgerald, S. V. and A. M. Connell. 2008. Group I adenovirus infections. In: Saif, Y.M. Diseases of Poultry. 12 thEds. Iowa, Wiley- Blackwell pp: 252-266.
8- Gomis, S., A. R. Goodhope, A. D. Ojkic and W. Philip. 2006. Inclusion body hepatitis as a primary disease in broilers in Saskatchewan, Canada. Avian diseases 50(4): 5-550.
9- Harrachm B., M. Benkö, G. W. Both, M. Brown, A. J. Davison, M. Echavarria, M. Hess, M. S. Jones, A. Kajon, H. D. Lehmkuhl, V. Mautner, S. K. Mittal and G. Wadell. 2012. Family Adenoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus Taxon. Ninth Rep. Int. Committtee Taxon. Viruses, 9th ed. Elsevier Academic Press, New York, p 125–141. 
10- Helmboldt, C. F. and M. N. Frazier. 1963. Avian hepatic inclusion bodies of unknown significance. Avian Diseases 7: 446-450.
11- Hess, M. 2000. Detection and differentiation of avian adenoviruses: a review. Avian pathology  29: 195-206
12- Hosseini, H. and R. Morshed. 2012. Molecular identification of fowl adenovirus associated with inclusion body hepatitis in Iran. Iranian Journal of virology 6(4): 7-12.
13- Khodakaram-Tafti, A., K. Asasi and F. Namazi. 2016. Clinicopathological characteristics of acute inclusion body hepatitis outbreak in broiler chickens in Iran. Bulg. Journal of Veterinary Medicine 19: 163-168.
14- King, A. M. Q., E. Lefkowitz, M. J. Adams and E. B. Carstens. 2012. Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses, 9th ed. Elsevier Academic Press, New York 41-125.
15- Morshed, R., H. Hosseini, A. G. Langeroudi, M. H. B. Fard and S. Charkhkar. 2017. Fowl adenoviruses D and E cause inclusion body hepatitis outbreaks in broiler and broiler breeder pullet flocks. Avian Diseases 61: 205-210.
16- Nateghi, E., J. Razmyar and M. R. Bassami. 2014. Molecular characterization of avian adenoviruses in Iranian broiler flocks. Iranian Journal of Veterinary Research 15: 164-167.
17- Rosenberger, J. K., R. J. Eckroade, S. Klopp and W. C. Krauss. 1974. Characterization of several viruses isolated from chickens with inclusion body hepatitis and aplastic anemia. Avian Diseases 18: 399-409.
18- Schachner, A., M. Matos, B. Grafl and M. Hess. 2018. Fowl adenovirus-induced diseases and strategies for their control–a review on the current global situation. Avian Pathology 47: 111-126.
19- Steer, P. A., D. O’sourke, S. A. Ghorashi and A. H. Noormohammadi. 2011. Application of high-resolution melting curve analysis for typing of fowl adenoviruses in field cases of inclusion body hepatitis. Australian Veterinary Journal 89(5):92-184.
20-Villate, D. 2001. Maladie des volailles. Eds. France. Agricole, p: 282
21- VonBulow, V., R. Rudolph and B. Fuchs. 1986. Folgen der Dopperlinfektion von kuken mit Adenovirus oder Reovirus und dem Erreger der Aviaren Infektiosen Anemia (CAA). Journal of Veterinary Medicine, Series B 33: 26-717.
 
Veterinary Research & Biological Products
Volume 36, Issue 3 - Serial Number 3
September 2023
Pages 69-76

Files

History

  • Receive Date: 02 January 2023
  • Revise Date: 07 March 2023
  • Accept Date: 07 March 2023

Share

How to cite

Statistics