Effect of mitochondria-targeted antioxidant Mito-TEMPO addition to the sperm herbal extender on ram chilled sperm quality

Document Type : Full Research Paper

Authors

Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

The aim of this study was to evaluate the sperm quality preservation in sheep using mitochondria-targeted antioxidant Mito-TEMPO during chilling process. In this experiment, semen samples were collected from five Zandi rams and added to the extender containing 0, 0.5, 1, 5, 50 and 500 µM Mito-TEMPO and stored at 4 ˚C until 48 hours. Sperm quality was evaluated at 0, 24 and 48 hours after chilling and total motility, progressive motility, viability, membrane integrity, mitochondrial activity and lipid peroxidation were assessed. No difference was observed between treatments at time 0 of cooling. During 24 and 48 hours cooling periods, using 5 and 50 µM Mito-TEMPO increased total motility, progressive motility, viability, membrane integrity, mitochondrial activity and decreased lipid peroxidation in ram chilled sperm. In conclusion, using 5 and 50 µM Mito-TEMPO in chilling herbal extender could be a suitable method to conserve ram sperm quality during chilling process. 

Keywords

References

1-Bucak, M.N. and N. Tekin. 2007. Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Small Ruminant Research. 73: 103–108.
2-Dikalova, A.E., Bikineyeva, A.T., Budzyn, K., Nazarewicz, R.R., McCann, L., Lewis, W., Harrison, D.G. and Dikalov, S.I., 2010. Therapeutic targeting of mitochondrial superoxide in hypertension. Circulation Research. 107: 106-116.
3-Dikalov, S., 2011. Cross talk between mitochondria and NADPH oxidases. Free Radical Biology and Medicine. 51: 1289-1301.
4- Hu, H. and Li, M., 2016. Mitochondria-targeted antioxidant mitotempo protects mitochondrial function against amyloid beta toxicity in primary cultured mouse neurons. Biochemical and biophysical research communications. 478:174-180.
5-Lu, X., Zhang, Y., Bai, H., Liu, J., Li, J. and Wu, B., 2018. Mitochondria-targeted antioxidant MitoTEMPO improves the post-thaw sperm quality. Cryobiology. 80:26-29.
6- Masoudi, R., Asadzadeh, N. and Sharafi, M. 2020. The mitochondria-targeted antioxidant Mito-TEMPO conserves rooster’s cooled semen quality and fertility potential. Theriogenology. 156: 236-241.
7- Masoudi, R., Asadzadeh, N. and Sharafi, M. 2021. Effects of freezing extender supplementation with mitochondria-targeted antioxidant Mito-TEMPO on frozen-thawed rooster semen quality and reproductive performance. Animal Reproduction Science. 225:106671.
8- Salmani, H., M.M. Nabi, H. Vaseghi-Dodaran, M.B. Rahman and et al. 2013. Effect of glutathione in soybean lecithin-based semen extender on goat semen quality after freeze-thawing. Small Ruminant Research. 112: 123–127.
9- Sharafi, M., M. Forouzanfar, S.M. Hosseini, M. Hajian and et al. 2009. In vitro comparison of soybean lecithin based-extender with commercially available extender for ram semen cryopreservation. International Journal of Fertility and Sterility. 3: 149–152.
10- Trnka, J., Blaikie, F.H., Smith, R.A. and Murphy, M.P., 2008. A mitochondria-targeted nitroxide is reduced to its hydroxylamine by ubiquinol in mitochondria. Free Radical Biology and Medicine. 44: 1406-1419.
11-Yang, S.G., Park, H.J., Kim, J.W., Jung, J.M., Kim, M.J., Jegal, H.G., Kim, I.S., Kang, M.J., Wee, G., Yang, H.Y. and Lee, Y.H., 2018. Mito-TEMPO improves development competence by reducing superoxide in preimplantation porcine embryos. Scientific Reports. 8: 1-10.
12-Zaniboni, L. and Cerolini, S., 2009. Liquid storage of turkey semen: Changes in quality parameters, lipid composition and susceptibility to induced in vitro peroxidation in control, n-3 fatty acids and alpha-tocopherol rich spermatozoa. Animal Reproduction Science. 112:51-65.
13- Zarei, F., Kia, H.D., Masoudi, R., Moghaddam, G. and Ebrahimi, M. 2021. Supplementation of ram's semen extender with Mito-TEMPO I: Improvement in quality parameters and reproductive performance of cooled-stored semen. Cryobiology. 98:215-218.
14-Zhang, X., Lu, X., Li, J., Xia, Q., Gao, J. and Wu, B., 2019. Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients. Cryobiology. 91:18-22.
Veterinary Research & Biological Products
Volume 36, Issue 4 - Serial Number 4
December 2023
Pages 14-19

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History

  • Receive Date: 25 December 2022
  • Accept Date: 01 January 2023

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