Evaluation of osmotic fragility of erythrocytes following injection of crude venom and extracted fractions of Hemiscorpius lepturus scorpion and the effect of polyvalent scorpion antivenom to prevent these changes

Document Type : Full Research Paper

Authors

1 Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization (AREEO), Iran

2 epartment of Venomous Animals and Toxins, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization (AREEO), Ahvaz, Iran.

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Abstract

Scorpion sting is one of the most important health problems in the world, including Iran. In this study, changes in osmotic fragility of erythrocytes following injection of crude venom and fractions of Hemiscorpius lepturus and evaluation of the efficacy of polyvalent antivenom to prevent these changes in rats. A total 96 rats, including the control and the second to eighth groups, received crude scorpion venom and fractions one to six, respectively. Then, blood samples were taken at 1, 3 and 24 hours after injection. Red blood cells were placed in media with different concentrations of sodium chloride and the amount of hemolysis was determined. The crude venom and the fraction two had reduced the resistance of erythrocytes to hypotonic concentrations. These two groups were challenged to evaluate the effect of antimony produced by Razi antivenom Institute. The results showed that in the groups received the crud venom and fraction 2, the resistance of red blood cells to hypotonic concentrations decreased and the curve of red blood cells changed. The fragility of red blood cells in these groups and compared with the control group showed a significant increase (p <0.05). The one-third fraction produced the least changes in the fragility of red blood cells. The level of erythrocyte fragility in the groups that received the scorpion sting antivenom did not show significant changes compared to the control group. (P> 0.05). Administration of scorpion sting antivenom has been able to significantly prevent changes in the extracellular osmotic curve of red blood cells.

Keywords

References

1. Afzali, N. And N. Pezeshki. 1998. Evaluation of acute renal failure due to gadium bite in children, Scientific, Medical Journal of Ahvaz University of Medical Sciences 25: 42 -48.
2. Atyabi, N. 1384. Clinical Veterinary Pathology: Laboratory Methods. First Edition, University of Tehran Press. 99-100.
3. Badhe R. V., A.B. Thomas, A.D. Deshpande, N. Salvin and A. Waghmare. 2007. The action of red scorpion (Mesobuthus tumulus Coconsis, Pocock) venom and its isolated protein fractions on blood sodium level. Journal of Venomous Animals and Toxins including Tropical Diseases 13(1):82-93. 
4. Chaubey, M. 2009. Antivenom reversal of biochemical alterations induced by black scorpion Heterometrus fastigiousus venom in mice, Journal Of venomous Animals and Toxins including tropical Diseases 15(2): 226-235.
5.  Chaubey, M. 2010. Changes in Different Blood Parameters during Mesobuthus tamulus gangeticus Pocock Envenomation.  Asian Journal of Applied Sciences. 3 (6): 411-416, 2010
6. Chitnis, P., A. Maraghi Sharif and B. Vazirianzadeh. 1372. Epidemiological and laboratory study of scorpion sting in Khuzestan, Journal of Guilan Medical School 8: 5-12.
7. Dehghani, R., T. Khamechian, T. Tirgari, S.Vatandoost, H. Rathi, Y. Rafinejad, J. Vousavi. 2005. The effect of gadium scorpion venom on white and red blood cells and rat hematocrit, Journal of Shahid Sadoughi University of Medical Sciences and Health Services 13 (1): 32 -41.
8. Dehghani, R. and N. Valaei. 2005. A Review of the Scorpion Situation and Its Problems in Iran. Feyz Scientific Research Quarterly 33: 66-84.
9. Farzan Pey, R. 2004.Scorpion bite and its consequences. Journal of Research and Construction 25: 123-125.
10. Godal, H.C., A.T. Elde, N. Nyborg, N. Brosstad. 1980. The normal range of osmotic fragility of red blood cells .Scand Journal Hematology 25(2):107-12.
11. Jalali, A., M.H. Pipelzadeh, R. Sayedian and E. Rowan. 2010. A review of epidemiological, clinical and in vitro physiological studies of envenomation by the scorpion Hemiscorpius lepturus (Hemiscorpiidae) in Iran. Toxicon 55: 173-179. 
12. Kaneko, JJ. 2008. Clinical Biochemistry of Domestic Animal. 6th ed. Academic Press, San Diego 287-330.
13. Possani, L. D., R.C. Rodriguez de la vega, 2006. Scorpion venom peptides. In: Kastin, A.J. (Ed.), In: Hand book of Biologically Active Peptides. Elsevier Inc.339 -354.
14. Radha Krishna Murthy, K., A. Zare. 2001.  The use of Antivenom reverses hematological and Osmotic Fragility changes of erythrocytes caused by Indian Red Scorpion Mesobuthus tamulus concanesis Pocock in experimental envenoming.  Journal of Venomous Animimal Toxns7 (1)
15.  Radmanesh, M. 1997. Study of scorpion bites in Khuzestan, Part 3, Bites, Hemiscorpius leptorus and its clinical study. Journal of Medicine - Treatment 160: 41-43.
16.  Razi Jalali, M., S.M. Jalali, H. Najafzadeh Varzi, and A.A. Shokraian. 2016. Investigation of the protective effect of polyvalent and quercetin antifungal on homogram changes and osmotic fragility of red blood cells after injection of Mesobuthus eupeus in rats - Journal Iranian Veterinary Medicine, 12 (1):
17.  Razi Jalali, M., S.R. Fatemi Tabatabaei, M. Ahmadizadeh, and H. Mohseni. 1396. Effects of Scorpion venom on Hemiascorpius leptorus on hemogram changes and fragility of red blood cells and the study of the role of polyvalent 
18. antinoma in rats - Iranian Journal of Veterinary Medicine 131: 41-52.
19. Salama, WM., KM. Sharshar. 2013. Surveillance study on scorpion species in Egypt and comparison of the crude venom protein profiles. The journal of Basic and Applied Zoology. 66: 76-86.
20. Shahbazzadeh, D., T. Abdollahi., K. Pooshang Bagheri, M. Hosseini-NejadChafi, F. Sasani, F. Torkashvand, G. Khalili, B. Vaziri. 2012 Purification of Haemolytic/Necrotic Protein from the Venom of Deathful Hemiscorpius lepturus Scorpion in Khuzestan Province. Iranian Journal of Infectious Diseases and Tropical Medicine 16: 55. 
21. Shirmardi, S.P., M. Shamsaei, M. Gandomkar, E. Saniei, M. Ghannadi, A. Zare. 2010. Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom. Journal of Venomous Animals and Toxins including Tropical Diseases
22. Suleiman, J., J. Zargan, F. Ebrahimi, A. Farahmandnejad, and A. Haji Beigi. 2002. Investigation of the role of Hemiscorpius leptorus scorpion venom in erythrocyte fragility, Kowsar Medical Journal 7(3): 185-189
23. Zare Mirkabadi, A. 1385. Scorpion stings (prevention), first aid and treatment; First edition; Teymourzadeh and Tabib Publishing Cultural Institute. 16(4): 639-646.
24. Zare Mirakabbadi, A., H. Zolfagharian, A. Hedayat, A, Jalali. 2007.  Clinical and biochemical manifestation produced by scorpion (Hemiscorpius lepturus) venom in experimental animals. Journal of Venomous Animals and Toxins including Tropical Diseases 13 (4) 
25. Valavi E., M.J. Alemzadeh Ansari. 2008. Hemolytic uremic syndrome following Hemiscorpius lepturus (scorpion) sting. Indian J Nephrol. 18:166-8. 
 
Veterinary Research & Biological Products
Volume 35, Issue 4 - Serial Number 4
December 2022
Pages 140-149

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History

  • Receive Date: 16 July 2022
  • Revise Date: 30 October 2022
  • Accept Date: 22 November 2022

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