Transcriptomic Study of lncRNAs in Native Isfahan and Ross Commercial Chicken

Document Type : Full Research Paper

Authors

1 Department of Animal Science, Animal Science and Food Technology Faculty, Agricultural Sciences and Natural Resources, University of Khuzestan, Mollasani, Iran

2 Department of Animal Science, Animal Science and Food Technology Faculty, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran

3 South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute (IFSRI), Ag-ricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran

Abstract

 Based on the RNA-seq method, a thorough set of information can be obtained from the identification of geneswhich the results  may be beneficial  in the genetics and breeding of livestock and poultry. So far, few studies have been reported regarding the use of this method in the evaluation of native poultry transcriptome. Considering the importance of lncRNAs in biological processes, the present study has investigated the lncRNAs from the transcriptome of native Isfahan  chicken and the commercial Ross 708 strain as well as differences  in their expression. In this study using next generation sequencing, 568 lncRNAs were identified, out of which the expression changes of 116 lncRNAs were meaningful (P-value ≤0.05). The results showed differently-expressed incRNAs which are associated with the biological pathways including histone and lysine methylation, myeloid leukocyte activation, positive regulation of cytokine production ,, , and regulation of lymphocyte activation. The analysis of molecular functions of the genes indicated the activity of non-membrane-spanning tyrosine kinase, protein methyltransferase, S-adenosylmethionine-dependent methyltransferase, and transfer of one-carbon groupsacting on a protein, and signaling receptor binding are enhanced meaningfully. Based on the results, lncRNAs that vary in expression between native and commercial groups are associated with immune and methylation pathways, and can be used as biomarkers.

Keywords

References

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History

  • Receive Date: 04 June 2022
  • Revise Date: 20 August 2022
  • Accept Date: 24 August 2022

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