Bioinformatic Analysis and Sequencing of OmpL1-LipL41 Leptospiral Fusion Protein as a New Candidate for Leptospirosis Vaccine

Document Type : Short Communication

Authors

1 Department of biology , falavarjan Branch , Islamic Azad University , Isfahan , Iran

2 Department of Microbiology, falavarjan Branch, Islamic Azad University, Isfahan, Iran

3 Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

4 Department of Human vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

5 Department of Immunology, Razi Vaccine and Serum Research Institute, Agricultural Research,Education and Extension Organization (AREEO), Karaj, Iran

Abstract

In recent years, due to advances in vaccine design, bioinformatic studies on Leptospira have undergone significant changes and advances. The aim of this study was to design a fusion protein based on OmpL1 and LipL41 surface proteins that have a conserved structure over time. Based on the previous field studies, the target proteins were examined and the OmpL1-LipL41 fusion protein was selected. After examining the chemical properties of the target proteins using appropriate servers, the initial structure of the fusion protein was determined and finally this protein was placed in the PET32a+ vector between the two enzymatic regions of HindIII and BamHI. The results revealed that the protein weighs 73 kDa and has 2097 bp and 610 amino acids. The isoelectric pH of the above protein is 7 and its solubility degree in the prokaryotic system is 100%. Finally, according to the above data, it is predicted that the designed construct structure has acceptable immunogenic properties and can be expressed to an acceptable and successful extent in prokaryotic systems and used for immunogenicity studies of Leptospira as a new candidate in the production of leptospirosis vaccine and in the design and manufacture of diagnostic kits.

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References

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History

  • Receive Date: 30 December 2021
  • Revise Date: 12 March 2022
  • Accept Date: 29 March 2022

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