Investigating the lethal effect of curcumin on hydatid cyst protoscolices in vitro

Document Type : Full Research Paper

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Hydatidosis is one of the most important parasitic diseases common to human beings and animals. Various  drugs are used to cure this disease.. Due to the side effects of the currently available drug treatments, efforts are being made to find effectual alternatives with fewer side effects. In this current research, curcumin, as an effective ingredient of turmeric, was examined on protoscolices of hydatid cysts in vitro. For this purpose, sheep livers infected with hydatid cysts  were obtained from Mashhad industrial slaughterhouse, the cyst protoscolices  were collected, and the viability of protoscolices  was determined by 0.1% eosin staining. To study scolicidal effects of curcumin, different concentrations of curcumin (40, 80, 160, 320 micromol ) along with positive control (Savlon) and negative control (normal saline) were applied on protoscolices  with 90% viability, 30, 60, 120, 240, 480 minutes after 37̊ C heat exposure . Subsequently, vital staining was done , and pictures were captured with a camera equipped with Tcapture software, finally, the viability of the protoscolices was calculated. All  the experiments were repeated four times. The scolicidal effects of curcumin concentrations of 160 and 320 μmol one hour after exposure were significant in comparison with the negative control group. Therefore, curcumin in the used form showed  protoscolicidal effects only in high doses. However, it may be possible  to increase the anti-protoscolices  effects of curcumin in future studies by  modifications or  combination with substances that increase its penetration and absorption.

Keywords


1- Almalki, E., E. M. Al-Shaebi, S. Al-Quarishy, M. El-Matbouli and A. A. S. Abdel-Baki. 2017. In vitro effectiveness of Curcuma longa and Zingiber officinale extracts on Echinococcus protoscoleces. Saudi Journal of Biological Sciences 24: 90-94.
2- Carmena, D. and G. A. Cardona. 2013. Canine echinococcosis: Global epidemiology and genotypic diversity. Acta Tropica 128: 441-460.
3- Cui, L., J. Miao and L. Cui. 2007. Cytotoxic effect of curcumin on malaria parasite Plasmodium falciparum: Inhibition of histone acetylation and generation of reactive oxygen species. Antimicrobial Agents and Chemotherapy 51: 488-494.
4- El-Sayed, N. M., K. A. Ismail, S. A. E. G. Ahmed and M. H. Hetta. 2012. In vitro amoebicidal activity of ethanol extracts of Arachis hypogaea L., Curcuma longa L. and Pancratium maritimum L. on Acanthamoeba castellanii cysts. Parasitology Research 110: 1985-1992.
5- Gutiérrez-Gutiérrez, F., L. Palomo-Ligas, J. M. Hernández-Hernández, A. Pérez-Rangel, R. Aguayo-Ortiz, A. Hernández-Campos, R. Castillo, S. González-Pozos, R. Cortés-Zárate, M. A. Ramírez-Herrera, M. L. Mendoza-Magaña and A. Castillo-Romero. 2017. Curcumin alters the cytoskeleton and microtubule organization on trophozoites of Giardia lamblia. Acta Tropica 172: 113-121.
6- Hosseini, A., F. Jaffary, G. R. Asghari, S. H. Hejazi and L. S. Bidabadi. 2012. In vitro effects of turmeric and licorice total extracts on L. Major promastigotes. Journal of Isfahan Medical School 29.
7- Maggiore, M. A., A. A. Albanese, L. B. Gende, M. J. Eguaras, G. M. Denegri and M. C. Elissondo. 2012. Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes. Parasitology Research 110: 1103-1112.
8- Maheshwari, R. K., A. K. Singh, J. Gaddipati and R. C. Srimal. 2006. Multiple biological activities of curcumin: A short review. Life Sciences 78: 2081-2087.
9- Mahmoudvand, H., E. S. Dezaki, F. Kheirandish, B. Ezatpour, S. Jahanbakhsh and M. F. Harandi. 2014. Scolicidal effects of black cumin seed (nigella sativa) essential oil on hydatid cysts. Korean Journal of Parasitology 52: 653-659.
10- Moazeni, M., S. Larki, M. J. Saharkhiz, A. Oryan, M. A. Lari and A. M. Alavi. 2014. In vivo study of the efficacy of the aromatic water of Zataria multiflora on hydatid cysts. Antimicrobial Agents and Chemotherapy 58: 6003-6008.
11- Moazeni, M. and A. Nazer. 2010. In vitro effectiveness of garlic (allium sativum) extract on scolices of hydatid cyst. World Journal of Surgery 34: 2677-2681.
12- Moazeni, M. and A. Roozitalab. 2012. High scolicidal effect of Zataria multiflora on protoccoleces of hydatid cyst: An in vitro study. Comparative Clinical Pathology 21: 99-104.
13- Morais, E. R., K. C. Oliveira, L. G. Magalhães, É. B. C. Moreira, S. Verjovski-Almeida and V. Rodrigues. 2013. Effects of curcumin on the parasite Schistosoma mansoni: A transcriptomic approach. Molecular and Biochemical Parasitology 187: 91-97.
14- Oyeyemi, O., O. Adegbeyeni, I. Oyeyemi, J. Meena and A. Panda. 2018. In vitro ovicidal activity of poly lactic acid curcumin-nisin co-entrapped nanoparticle against Fasciola spp. eggs and its reproductive toxicity. Journal of Basic and Clinical Physiology and Pharmacology 29: 73-79.
15- Qian, W., H. Wang, D. Shan, B. Li, J. Liu and Q. Liu. 2015. Activity of several kinds of drugs against Neospora caninum. Parasitology International 64: 597-602.
16- Rouhani, S., N. Salehi, M. Kamalinejad and F. Zayeri. 2013. Efficacy of Berberis vulgaris aqueous extract on viability of Echinococcus granulosus protoscolices. Journal of Investigative Surgery 26: 347-351.
17- Saleheen, D., S. A. Ali, K. Ashfaq, A. A. Siddiqui, A. Agha and M. M. Yasinzai. 2002. Latent activity of curcumin against leishmaniasis in vitro. Biological and Pharmaceutical Bulletin 25: 386-389.
18- Sharma, R. A., A. J. Gescher and W. P. Steward. 2005. Curcumin: The story so far. European Journal of Cancer 41: 1955-1968.
19- Tiwari, B., R. Pahuja, P. Kumar, S. K. Rath, K. C. Gupta and N. Goyal. 2017. Nanotized curcumin and miltefosine, a potential combination for treatment of experimental visceral leishmaniasis. Antimicrobial Agents and Chemotherapy 61.