Seroprevalence of Linguatula serrata infection in goats from Ilam province using an in-house ELISA test

Document Type : Full Research Paper

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz,, Ahvaz, Iran

3 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz,, Iran

Abstract

Linguatula serrata is the cause of linguatolosis, a parasitic disease common to humans and animals. Canines and ruminants are important final and intermediate hosts for the parasite respectively. The objective of this study was to determine the seroprevalence of Linguatula serrata infection in goats of different regions in Ilam province by using the enzyme-linked immunosorbent assay (ELISA). The ELISA test was designed to detect Linguatula serrata infection in goats using excretory-secretory antigens, serum samples (62 negative and 40 positive) and anti-goat IgG conjugate.  A total of 805 goat serum samples collected from five regions of Ilam province were tested by ELISA.
Seroprevalence of parasite infection among goats in the province of Ilam was 28.57 percent, along with 65.8% in Ilam (center), 63.4% in Ivan (north), 19.2% in Dehloran (south), 16.9% in Chardavol (east), and 9.9% in Mehran (west).
The seroprevalence varied significantly between different areas of the province (p<0.01). There was no significant difference between the rate of infection in age groups 1- <2, 2- 3>, 3- ≥4 with 27.7%, 29.5%, 28.3%, respectively (P = 0.849), and also in resident (29.1%) and nomadic (26.9%) herds were not observed (P=0.545).
The results of this study indicate that the ELISA test can be used as one of the convenient serological methods to detect Linguatula serrata infection in goats. To control and manage the disease, educational and preventive measures are recommended due to the presence of infection in livestock of the region.

Keywords

References

1-Alborzi, A.  and T. Darakhshandeh. 2008. A survey on infection of Linguatula serrata nymphs in sheep slaughtered at Yasuj abattoir. Iranian Veterinary Journal 4(1):103-109    
2-Alborzi, A.R., M. Ghorbanpoor., H. Hamidinejat., M. Poormehdi Boroujeni and A. Mehdizadeh. 2015. Development and evaluation of an indirect ELISA test for serodiagnosis of Linguatula serrata infection in sheep. Iranian Veterinary Journal 11(1):24-33.  
3- Alborzi, A.R., M. Ghorbanpoor., H. Hamidinejat., S. Chamanara and S. Noori. 2016. Seroprevalence of Linguatula serrata nymph infection in sheep from west of Iran by ELISA. Iranian Veterinary Journal 12(2):1-9. 
4- Hassanzadeh khanbaghi, A., Sh. Ranjbar bahadori. And N. Hooghogi Rad. 2013. Survey of linguatulosis occurrence in small ruminants Slaughtered in Shahriyar Abattoir. Comparative Pathology, Journal of Islamic Azad University, Tehran 10(4): 1059-1064. 
5-Alborzi, A.R., P. Haddad Molayan and M. Akbari. 2013. Prevalence of Linguatula serrata Nymphs in Mesenteric Lymph Nodes of Cattle and Buffaloes Slaughtered in Ahvaz Abattoir, Iran. Iranian Journal of Parasitology 8(.2): 327-332.
6-Acha, P.N. and B. Szyfre. 2003. Zoonoses and communicable diseases common to man and animals (Parasitosis). PP. 345-380, Pan American Health Organization. Washington, DC, 
7- Anaraki Mohammadi, G., I. Mobedi., M. Ariaiepour., Z. Pourmohammadi and M. Zare Bidaki. 2008. A Case Report of Nasopharyngeal Linguatuliasis in Tehran, Iran and Characterization of the Isolated Linguatula serrata. Iranian Journal of Parasitology 3(1):53-5.
8- Bahrami, A.M., S. Yousofzadeh. and A. Kermanjani. 2010. Study of Linguatula serrata infection rate among shepherd and stray dogs in Ilam (western Iran). Scientific Journal of Ilam University of Medical Sciences19: 60-65.
9-Bowman, D.D., R.C Lynn. and M.L. Eberhard. 2003. Georgis Parasitology for Veterinarians. PP: 76-77. In: (8th ed). Elsevier Science, USA. 
10- Esmail-Nia K., S. Hadizadeh-Moalem., A. Derakhshanfa and G. Moatamedi. 2000. A study on the prevalence of Linguatula serrata infestation in small ruminants of Mazandaran Province in Babol abattoir. Pajouhesh and Sazandegi 54: 94–97. (in Farsi)
11- Garedaghi, Y. (2011). Prevalence of Linguatula serrata Nymph in Goat in Tabriz, North-West of Iran. Veterinary Research Forum 2 (2):129 –133.
12- Goddard, P., P. Bates. and K.A. Webster. 1999. Evaluation of a direct ELISA for the serodiagnosis of Osterus ovis infection in sheep. Veterinary Record 12(144):497-501.
13- Koehsler M., J. Walochnik., M. Georgopoulos., C. Pruente., W. Boeckeler., H. Auer and T. Barisani-Asenbauer. 2011. Linguatula serrata tongue worm in human eye, Austria. Emerging Infectious Diseases 17(5): 870-872.
14- Maleky, F. 2001. A case report of Linguatula serrata in human throat from Tehran, central Iran. Indian Journal of Medicine Science 55:439-41.
15- Meshgi, B. and O. Asgarian. 2009. Prevalence of Linguatula serrata Infestation in Stray Dogs of Shahrekord, Iran. Journal of Veterinary Medicine, Series B 50(9): 466–467.
16-Nourollahi-Fard, S. R., R. Kheirandish., E. Norouzi-Asl. and S. Fathi. 2010.The prevalence of Linguatula serrata nymphs in goats slaughtered in Kerman slaughterhouse, Kerman, Iran. Veterinary Parasitology171(1-2):176–178.
17- Oryan, A., S. Sadjjadi., D. Mehrabani and M. Rezaei. 2007. The status of Linguatula serrata infection of stray dogs in Shiraz, Iran. Comparative Clinical Pathology17(1):55-60.
18 –Saumya, Pal, S., M. Bhargava., A. Kumar., N. Mahajan., S. Das., K. Nandi., S. Guha., M. Raman., N. Jeyathilakan. and J. Biswas. 2011. An unusual intraocular tongue worm in anterior chamber: a case report. Ocular Immunology and Inflammation 19(6):442-443. 
19- Razavi, SM., SS. Shekarforoush and M. Izadi. 2004. Prevalence of Linguatula serrata nymph in goats in Shiraz, Iran. Small Ruminant Research 54: 213–217.
20- Rezaei, F., M. Tavassoli and A. Mahmoudian. 2011. Prevalence of Linguatula serrata infection among dogs (definitive host) and domestic ruminants (intermediate host) in the North West of Iran. Veterinarni Medicina 56 (11): 561–567.
21- Sadjjadi, S.M., S.M. Ardehali and A. Shojaei. 1998. A case report of Linguatula serrata in human pharynx from Shiraz, Southern Iran. Medical Journal of Islamic Republic of Iran12:193-194.
22-  Schmidt, G.D. and L.S. Roberts. 2000. Foundation of Parasitology. (6th ed.). McGraw- Hill International Editions, Singapore. pp: 485 - 490. 
23- Siavashi M. R., M. Assmar and A. Vatankhah. 2002. Nasopharyngeal pentastomiasis (Halzoun): report of 3 cases. Iranian Journal of Medical Sciences 27(4):191-192.
24-Soulsby, E.J.L. 1982. Helminths, Arthropods & Protozoa of domesticated Animals. (7th ed.). Bailliere Tindall and Cassell Ltd, London. pp: 497-498.
25-Tappe, D. and D.W. Buttner. 2009. Diagnosis of human visceral pentastomosis, PLOS Neglected Tropical Diseases 3(2):320.
26- Tappe, D., R. Winzer., D. W. Buttner., P. Strobel., A. Stich., H. Klinker and M. Frosch. 2006. Linguatuliasis in Germany. Emerging infectious diseases 12(6):1034-6.
27- Tavassoli M., H. Tajik., B. Dalir-Naghadeh. and H. Lotfi. 2007. Study of Linguatula serrata infestation in mesenteric lymph nodes of goats in slaughterhouse of Urmia, Iran. Iranian Veterinary Journal 3: 85–90.
28.Valinejad MR., H. Shokrani. and Gh. Farjanikish. 2019. Frequency, pathology and public health importance of linguatulosis in goats slaughtered in Lorestan province abattoirs, Veterinary Researches & Biological Products 125: 51-58.
29-Yilmaz H., Z. T. Cengiz., M. Cicek. and A.C. Dulger. 2011. A nasopharyngeal human infestation caused by Linguatula serrata nymphs in Van province: a case report. Turkiye Parazitol Derg 35:47-9.
 
Veterinary Research & Biological Products
Volume 35, Issue 4 - Serial Number 4
December 2022
Pages 52-58

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History

  • Receive Date: 26 September 2021
  • Revise Date: 03 March 2022
  • Accept Date: 07 March 2022

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