The effect of pollen cake feeding on the quantity and quality of Royal Jelly produced at 24, 48, 72 hours after larval transplantation in beehives

Document Type : Full Research Paper

Authors

1 Department of Animal Sciences, Sari Agricultural Sciences and Natural Resources University, Mazandaran, Iran.

2 Department of Animal Sciences, Sari Agricultural Sciences and Natural Resources University, Mazandaran, Iran

3 Animal Science Research Institute of Iran,, Agricultural Research,Education and Extension Organization, Karaj, Iran

Abstract

The aim of this study was to use pollen cake on the quantity and quality of royal jelly (RJ) produced at 24, 48 and 72 hours after larval transplantation in beehives. The statistical design used in a completely randomized form was factorial in which the consumption and non-consumption of pollen cake and harvest times (24, 48 and 72 hours after larval transplantation) were main factors. The amount of RJ harvested in each pole was evaluated. Percentage of HDA-10, crude protein and moisture were measured to measure quality. The results showed that the quantity of RJ (g) was affected by harvest time, and the interaction of harvest time and cake feeding. The highest amount of RJ was seen in the treatment of cake feeding and harvest time 72. The percentage of HDA-10 was affected only by harvest time and its maximum value was at 24 harvest time. The percentage of moisture, protein and fat of RJ were not affected by the harvest time, cake feeding and the interactions. However, the percentage of sugars (glucose and fructose) and total phenol were affected by different harvest times and the interactions. Consumption of pollen cake has increased the harvest time of Royal Jelly for at least 20 days. In conclusion, the results of the present study showed that due to the cost of gel removal in 24 hours and its low amount and high quality, the price of 24-hour Royal Jelly was estimated to be at least 2.5 times the price of regular gel. 

Keywords


1. Al-Kahtani, S.N., E.K. Taha, K.A. Khan, M.J. Ansari, S.A. Farag, D.M. Shawer and E.S.M. Elnabawy. 2020. Effect of harvest season on the nutritional value of bee pollen protein. PloS one. 15(12): e0241393.
2. Cobey, S. 2005a. A versatile queen rearing and banking. System, part I: The Cloake board method of queen rearing. American Bee Journal. 4:308-311. 
3. Cobey, S. 2005b. A versatile queen rearing and banking system, part II: Conclusion use of the Cloake board for banking purposes. American Bee Journal. 5: 385-386
4. Flanjak, I., M. Jakovljević, D. Kenjerić, M. Cvijetić Stokanović, L. Primorac and B. Bilić Rajs. 2017. Determination of (2E)-10-hydroxydec-2-enoic acid in Croatian royal jelly by high-performance liquid chromatography. Croatian Journal of Food science and Technology. 9 (2):152-157.
5. Garcia-Amoedo, L.H. and L. B. D. Almeida-Muradian. 2003. Determination of trans-10-hydroxy-2-decenoic acid (10-HDA) in royal jelly from São Paulo State, Brazil. Food Science and Technology. 23:62-65
6. Jie, H., P. M. Li, G.J. Zhao, X.L. Feng, D.J. Zeng, C.L. Zhang and Q. Chen. 2016. Amino acid composition of royal jelly harvested at different times after larval transfer. Genetics and Molecular Research. 15(3):110-119.
7. Kamyab, S., M. Gharachorloo, M. Honarvar and M. Ghavami. 2020. Quantitative analysis of bioactive compounds present in Iranian royal jelly. Journal of Apicultural Research. 59: 42-52
8. Karacaoğlu, M., A.U. Koç, B. Bakır, K. Metin, B. Keser, and B. Birincioğlu. 2019. The effect of harvest time and number of queen cell on 10-HDA and total protein content in royal jelly. International Animal Science conference, Cappadocia, Turkey, 
9. Kumar, J. 2015. Impact of Harvesting Time on Royal Jelly Production in Apis mellifera L. Proceedings of UGC Sponsored National Seminar on ‘Innovative Approaches for Sustainable Agriculture, Livelihood and Environment Security. Rome, Italy
10. Ma, C., L. Zhang, M. Feng, Y. Fang, H. Hu, B. Han, L. Meng and J. Li. 2021. Metabolic profiling unravels the effects of enhanced output and harvesting time on royal jelly quality. Food Research International. 139:109974.
11. Mohebodini, H., Gh. Tahmasebi, B. Dastar, Y. Jafari-Ahangari and S. Zerehdaran. 2016. The hypopharyngeal glands development in Iranian honey bee workers fed with different level of thiamin.  Animal Science Journal. 110:212-223 (In Farsi).
12. Pattamayutanon, P., C. Peng, C. Sinpoo and P. Chantawannakul. 2018. Effects of Pollen Feeding on Quality of Royal Jelly. Journal of Economic Entomology. 111(6):2974-2978
13. Sadredini, A., Gh. Nehzati Paghale and F. Ghaziani. 2015. Growth hypopharyngeal glands and factors affecting the activity of the worker bees. Quarterly Journal of Bee Science and Technology 11:19-24 (In Farsi).
14. Şahinler, N., A. Gül and A. Şahin. 2005. Vitamin E supplement in honey bee colonies to increase cell acceptance rate and royal jelly production. Journal of Apicultural Research. 44:58-60.
15. Sereia, M.J., V.A. Arnaut de Toledo, M.C. Ruvolo-Takasusuki, E.S. Sekine, P. Faquinello and F. M. Costa-Maia. 2010. Financial viability of the royal jelly production with Africanized honeybees supplemented with different nutrients, in Portuguese. Acta Scientiarum Animal Sciences. 32:467–474. 
16. Steen, J. 2007. Effect of home-made pollen substitute on honey bee colony fevelopment. Journal of Apicultural Research. 46:114-119.
17. Van Toor, R. F. 2006. Producing Royal Jelly: A Guide for the Commercial and Hobbyist Beekeeper. pp. 180-220, In: How To Content Press, Begbroke. United Kingdom
18. Wu, X., F. Zhang, C. Guan, P. Qi-Zhong, Z. Lin-Bin, Y. Wei-Yu and Z. Zhi-Jiang. 2015. A new method of royal jelly harvesting without grafting larvae. Entomological News. 124:277-281.
19. Zheng, H.Q., F.L. Hu and V. Dietemann. 2011. Changes in composition of royal jelly harvested at different times: consequences for quality standards. Apidologie. 42(1): 39-47.