Evaluation of antibiotic resistance of Staphylococcus aureus isolated from bovine mastitis

Document Type : Full Research Paper

Authors

Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Kurdistan, Iran

Abstract

Mastitis is one of the most important diseases in the livestock industry, which causes many economic losses. Staphylococcus aureus is one of the infectious agents of bovine mastitis. Due to the prevalence of antibiotic resistance in isolates of this bacterium, the aim of this study was to determine the antibiotic resistance pattern of bovine mastitis S. aureus isolates. This study was conducted in 7 industrial farms around Hamedan. The amount of 100 μl milk samples were cultured on the specific and differential culture media and according to the characteristics of S. aureus on these media, the bacteria were isolated. Isolated bacteria were confirmed by PCR test for genus and species specific gene (femA). Then, the antibiotic resistance patterns to different antibiotics were investigated by disk diffusion method and the results were reported according to the CLSI standard. In the present study, from a total of 280 milk samples taken from 7 industrial farms, a total of 53 (18.92%) S. aureus bacteria were isolated. Based on the results, the highest resistance was related to penicillin (100% resistant) and the highest sensitivity was related to the vancomycin (2 cases resistant (3.7%)). The results of this test showed that out of 53 bacteria, 7 (13.20%) had intermediate resistance to vancomycin. The report of high resistance to common antibiotics in this study is very important. Also, the observation of 7 cases of intermediate resistance and one case of complete resistance to vancomycin is a serious warning for the emergence of vancomycin-resistant animal strains.

Keywords

References

1- Bradley, A. J. 2002. Bovine mastitis: an evolving disease. The Veterinary Journal 164: 116-128.
2- Brakstad, O. G., K. Aasbakk and J. A. Maeland. 1992. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. Journal of Clinical Microbiology 30: 1654-1660.
3- Cheng, W. N. and S. G. Han. 2020. Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments—A review. Asian-Australasian Journal of Animal Sciences 33: 1699.
4- Cockerill, F. R. and M. Wikler. 2012. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing: twentysecond informational supplement;[ provides updated tables for M02-A11 and M07-A9].
5- De Oliveira, A., J. Watts, S. Salmon and F. M. Aarestrup. 2000. Antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis in Europe and the United States. Journal of Dairy Science 83: 855-862.
6- Hosseinpour Feizi, M. A., G. Zarrini and M. Tahmasebi. 2013. Evaluation of Protein A Gene Tandem Repeat Polymorphism of Staphylococcus aureus Isolated From Bovine Mastitis in Tabriz. Jundishapur Journal of Microbiology 6.
7- Khodadadi, P., M. Bizhanzadeh, A. Najafi, V. Zarinpour, A. Moshfe and H. Ansari. 2016. Antibiotic Resistance and Detection of femA Gene in Staphylococcus Aureus Isolates from Raw Milk. Medical Laboratory Journal 10: 40-45.
8- Kobayashi, N., H. Wu, K. Kojima, K. Taniguchi, S. Urasawa, N. Uehara, Y. Omizu, Y. Kishi, A. Yagihashi and I. Kurokawa. 1994. Detection of mecA, femA, and femB genes in clinical strains of staphylococci using polymerase chain reaction. Epidemiology & Infection 113: 259-266.
9- Li, T., H. Lu, X. Wang, Q. Gao, Y. Dai, J. Shang and M. Li. 2017. Molecular characteristics of Staphylococcus aureus causing bovine mastitis between 2014 and 2015. Frontiers in Cellular and Infection Microbiology 7: 127.
10- Li, X., Y. Xiong, X. Fan, P. Feng, H. Tang and T. Zhou. 2012. The role of femA regulating gene on methicillin-resistant Staphylococcus aureus clinical isolates. Medecine et Maladies Infectieuses 42: 218-225.
11- Quinn, P. J., B. K. Markey, F. C. Leonard, P. Hartigan, S. Fanning and E. Fitzpatrick. 2011. Veterinary Microbiology and Microbial Disease. John Wiley & Sons.
12- Schmidt, T., M. M. Kock and M. M. Ehlers. 2017. Molecular characterization of Staphylococcus aureus isolated from bovine mastitis and close human contacts in South African dairy herds: genetic diversity and inter-species host transmission. Frontiers in Microbiology 8: 511.
13- Shamakhi, M., F. Mousakhani, M. Arjomandzadegan and S. Dezfouli. 2013. Study on antibiotic resistance and frequency of some genes of biofilm production in staphylococcus aureus isolated from mastitis of cow. Journal of Veterinary Clinical Research 3: 225-234.
14- Weese, J. S. 2010. Methicillin-resistant Staphylococcus aureus in animals. ILAR journal 51: 233-244.
15- Shamakhi, M., F. Mousakhani, M. Arjomandzadegan, and S. Dezfouli. 2013. Study on antibiotic resistance and frequency of some genes of biofilm production in staphylococcus aureus isolated from mastitis of cow. Journal of Veterinary Clinical Research 225-234.
16- Bahrami, A., Shamsi, M., BAHRAMI, A., Soltani, S., Shokri, A., Ahmadi, M., Talebimaymand, F. and Abasian, L., 2016. Staphylococcus aureus isolated from bovine mastitis milk livestock and the study of antibiotic resistance. Journal of Ilam University of Medical Sciences 24: 18-26.
Veterinary Research & Biological Products
Volume 35, Issue 4 - Serial Number 4
December 2022
Pages 68-73

Files

History

  • Receive Date: 07 December 2021
  • Revise Date: 12 January 2022
  • Accept Date: 22 January 2022

Share

How to cite

Statistics