A survy of Escherichia coli Contamination in eggs of Ardabil and determination of their Antibiotic Resistance

Document Type : Full Research Paper

Author

Poulry Diseases, Meshginshahr Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

Escherichia coli is causative agent of colibacillosis, which contaminated eggs are important sources of of spread of this disease. Antimicrobial therapy is an important way to control and reduce this disease. But in recent years, the widespread use of antibiotics has led to the proliferation of resistant genes and increased antibiotic resistance among bacterial strains. Increasing antibiotic resistance is currently a global health problem. The aim of this study was to determine contaminated rate of Escherichia coli in native and industrial poultry eggs of Ardabil city and to evaluate their drug resistance. 250 eggs including 81 native eggs, 86 bulk eggs and 83 labeled industrial eggs were randomly collected from retail stores in Ardabil during a period of 6 months and were examined in terms of E.coli contamination by standard culture methods. Biochemical tests and PCR were used for definitive diagnosis of strains. Then, antibiotic resistance was determined by disk diffusion method. From 250 samples examined, five isolates (2%) were identified, of which three isolates (1.20%) were obtained from the egg shells and two isolates (0.80%) from the egg contents. 2.47% (two cases)of native egg shells and 1.16% (one case) of bulk egg shells were contaminated with E. coli. 1.23% (one case)  of native egg contents and 1.16% (one case) of bulk egg contents had E. coli contamination. E. coli was not isolated in the shell and contents of industrial eggs. In the study of antibiotic resistance pattern of isolated strains, the highest resistance was observed to neomycin, chloramphenicol, nalidixic acid, lincomycin and amoxicillin and the lowest resistance was to kanamycin, ceftazidium and cephalexin. The results of this study showed that native eggs and bulk eggs are E.coli contamination, which is necessary to pay attention to their hygienic maintenance and cooking. Also, due to the high antibiotic resistance of the isolates, the excessive use of antibiotics in poultry farms should be prevented.

Keywords

References

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Veterinary Research & Biological Products
Volume 34, Issue 4 - Serial Number 4
January 2022
Pages 112-120

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History

  • Receive Date: 31 August 2020
  • Revise Date: 11 October 2020
  • Accept Date: 12 October 2020

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