Molecular detection and prevalence of B. bigemina and B. bovis in cattle in Lorestan province, Iran

Document Type : Full Research Paper

Authors

1 Department of Parasitic Disease Research, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

2 Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural ‎Research Education and Extension Organization (AREEO), Shiraz, Iran.‎

Abstract

Babesiosis is a tick-borne disease caused by protozoa of the genus Babesia. This study aimed to determine the B. bigemina and B. bovis among cattle of Lorestan Province. A total of 258 blood samples were collected via the jugular vein from healthy cattle, randomly. The extracted DNA from blood cells was amplified by Babesia-all primers, which amplify an approximately 400bp DNA fragment from the region of the 18S rRNA gene from various members of the genus Babesia. All cattle positive samples were further analysed for the presence of B. bigemina and B. bovis by specific semi-nested PCR. B. bigemina and B. bovis were identified by specific semi-nested PCR in 17.8% and 19.4% of cattle blood samples, respectively. Chi-square tests were used to compare molecular prevalence values relative to climate, altitude, longitude, latitude,  farm type, hygiene, vectors, use of acaricide, distance from other farms, contact with wild ruminants, farm density, race, age, and sex were recorded for each animal. Among these factors, a significant association was only found between the prev-alence of B. bigemina infection and farm density (P< 0.05). Multivariable logistic regression analysis revealed that farm type and farm density were significant risk factors for B. bovis infection in cattle of Lorestan province (P< 0.01). The results of this study can be used in strategic planning for the prevention and control of bovine babesiosis in dairy cattle in the west of Iran.

Keywords


1- Abdela, N., N. Ibrahim and F. Begna. 2018. Prevalence, risk factors and vectors identification of bovine anaplasmosis and babesiosis in and around Jimma town, Southwestern Ethiopia. Acta Tropica. 177: 9-18.
2- Aktas, M. and S. Ozubek. 2015. Molecular and Parasitological survey of bovine piroplasms in the Black Sea region, including the first report of babesiosis associated with Babesia divergens in Turkey. Journal of Medical Entomology. 52: 1344-1350.
3- Amorim, L. S., A. A. Wenceslau, F. S. Carvalho, P. L. S. Carneiro and G. R. Albuquerque. 2014. Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil. Revista Brasileira de Parasitologia Veterinária. 23: 328-336.
4- Bhat, S., H. Singh, N. Singh and S. Rath. 2015. Molecular detection of Babesia bigemina infection in apparently healthy cattle of central plain zone of Punjab. Journal of Parasitic Diseases 39: 649-653.
5- Bloch, E. M., T. H. Lee, P. J. Krause, S. R. Telford III, L. Montalvo, D. Chafets, S. Usmani-Brown, T. J. Lepore and M. P. Busch. 2013. Development of a real time polymerase chain reaction assay for sensitive detection and quantitation of Babesia microti infection. Transfusion 53: 2299-2306.
6- Bock, R., L. Jackson, A. De Vos and W. Jorgensen. 2004. Babesiosis of cattle. Parasitology 129: S247-S269.
7- Chaudhry, Z., M. Suleman, M. Younus and A. Aslim. 2010. Molecular detection of Babesia bigemina and Babesia bovis in crossbred carrier cattle through PCR. Pakistan Journal of Zoology 42.
8- Cheshti, B., G. Razmi and A. NaghibI. 2013. A comparative study on haemoprotozoa infection in apparently healthy cattle in different geographical areas of Iran using PCR method.
9- Dantas-Torres, F., L. C. Alves and G. Uilenberg. Section. 2017. Babesiosis.  347-354.  Arthropod Borne Diseases. Springer; 
10- Fakhar, M., A. Hajihasani, S. Maroufi, H. Alizadeh, H. Shirzad, F. Piri and A. S. Pagheh. 2012. An epidemiological survey on bovine and ovine babesiosis in Kurdistan Province, western Iran. Tropical Animal Health and Production 44: 319-322.
11- Farooqi, S. H., M. Ijaz, M. I. Rashid, A. I. Aqib, Z. Ahmad, M. H. Saleem, K. Hussain, S. Islam, H. Naeem and A. Khan. 2017. Molecular epidemiology of Babesia bovis in bovine of Khyber Pakhtunkhwa, Pakistan. Pak Vet J 37: 275-280.
12- Georges, K., G. R. Loria, S. Riili, A. Greco, S. Caracappa, F. Jongejan and O. Sparagano. 2001. Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily. Veterinary Parasitology. 99: 273-286.
13- Ghadimipour, R., V. Noaman and M. Taghizadeh. 2020. Prevalence and risk factors of Babesia bovis and Babesia bigemina infection in cattle in northwestern Iran. Veterinary Clinical Pathology .14: 155-168.
14- Gubbels, J., A. De Vos, M. Van der Weide, J. Viseras, L. Schouls, E. De Vries and F. Jongejan. 1999. Simultaneous Detection of BovineTheileria and Babesia Species by Reverse Line Blot Hybridization. Journal of Clinical Microbiology. 37: 1782-1789.
15- Hashemi-Fesharki, R. and A. Amjadi. 1977. Outbreak of Babesia bovis infection in cattle and its control. Archives of Razi Institute. 29: 83-86.
16- Hussain, S., K. Ashraf, N. Anwar, M. Jamal, H. Naeem, N. Ahmad and A. Rahman. 2017. Diagnosis of babesia bovis infection in indigenous and crossbred cattle with comparison between conventional and molecular diagnostic techniques. J Inf Mol Biol. 5: 1-6.
17- Ibrahim, O., Z. Taha and S. Jassim. 2012. Prevalence of Babesia bovis in cattle in Tikreet city and its surroundings with hematological study. Tikrit Journal of Pure Science. 17: 32-34.
18- Jirapattharasate, C., P. F. A. Moumouni, S. Cao, A. Iguchi, M. Liu, G. Wang, M. Zhou, P. Vudriko, T. Changbunjong and S. Sungpradit. 2016. Molecular epidemiology of bovine Babesia spp. and Theileria orientalis parasites in beef cattle from northern and northeastern Thailand. Parasitology International. 65: 62-69.
19- Kalani, H., M. Fakhar and A. Pagheh. 2012. An overview on present situation babesiosis and theileriosis and their distribution of ticks in Iran. Iranian Journal of Medical Microbiology. 5: 59-71.
20- Khamesipour, F., A. Doosti, A. Koohi, M. Chehelgerdi, A. Mokhtari-Farsani and A. A. Chengula. 2015. Determination of the presence of Babesia DNA in blood samples of cattle, camel and sheep in Iran by PCR. Arch Biol Sci Belgrade. 63: 83-90.
21- Motavalli-Haghii, M., F. Etemadifar, M. Fakhar, S. H. Teshnizi, M. Soosaraei, A. Shokri, A. Hajihasani and H. Mashhadi. 2017. Status of babesiosis among domestic herbivores in Iran: A systematic review and meta-analysis. Parasitology Research. 116: 1101-1109.
22- Muhanguzi, D., E. Matovu and C. Waiswa. 2010. Prevalence and characterization of Theileria and Babesia species in cattle under different husbandry systems in western Uganda. International Journal of Animal and Veterinary Advances. 2: 51-58.
23- Nayel, M., K. M. El-Dakhly, M. Aboulaila, A. Elsify, H. Hassan, E. Ibrahim, A. Salama and T. Yanai. 2012. The use of different diagnostic tools for Babesia and Theileria parasites in cattle in Menofia, Egypt. Parasitology Research. 111: 1019-1024.
24- Niu, Q., Z. Liu, P. Yu, J. Yang, M. O. Abdallah, G. Guan, G. Liu, J. Luo and H. Yin. 2015. Genetic characterization and molecular survey of Babesia bovis, Babesia bigemina and Babesia ovata in cattle, dairy cattle and yaks in China. Parasites & Vectors. 8: 518.
25- Noaman, V. 2013. A molecular study on Theileria and Babesia in cattle from Isfahan province, Central Iran. Journal of Parasitic Diseases. 37: 208-210.
26- Noaman, V. 2014. Comparison of molecular and microscopic technique for detection of Theileria spp. in carrier cattle. Journal of Parasitic Diseases. 38: 64-67.
27- Noaman, V., M. Abdigoudarzi and A. Nabinejad. 2017. Abundance, diversity, and seasonal dynamics of hard ticks infesting cattle in Isfahan Province, Iran. Archives of Razi Institute. 72: 15-21.
28- Noaman, V., R. Ghadimipour and R. Nabavi. 2021. First report of Babesia occultans in two symptomatic cows in Iran. Parasitology Research. 120: 1915-1919.
29- Noaman, V., A. A. Jahangirnejad and A. Nabinejad. 2005. A study on prevalence and identification of Babesia spp. in immigrant and sheep & goats and nomadic people of Isfahan Province. Pajouhesh – Va- sazandegi. 67: 35-41 (In Persian).
30- OIE. Section. 2012. Bovine anaplasmosis, Section 2.4. Bovinae.  589-600.  World Organization for Animal Health OIE terrestrial manual  Paris, France.
31- Rajabi, S., B. Esmaeilnejad and M. Tavassoli. 2017. A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran.  Veterinary Research Forum. Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. p. 299-306.
32- Ristic, I. and I. McIntyre. 2013. Diseases of Cattle in the Tropics: Economic and Zoonotic Relevance. illustrated ed.  Springer Netherlands.
33- Romero-Salas, D., A. Mira, J. Mosqueda, Z. García-Vázquez, M. Hidalgo-Ruiz, N. A. O. Vela, A. A. P. de León, M. Florin-Christensen and L. Schnittger. 2016. Molecular and serological detection of Babesia bovis-and Babesia bigemina-infection in bovines and water buffaloes raised jointly in an endemic field. Veterinary Parasitology. 217: 101-107.
34- Schnittger, L., A. E. Rodriguez, M. Florin-Christensen and D. A. Morrison. 2012. Babesia: a world emerging. Infection, Genetics and Evolution. 12: 1788-1809.
35- Sevgili, M., A. Cakmak, A. Gokcen, M. G. Altas and G. Ergun. 2010. Prevalence of Theileria annulata and Babesia bigemina in cattle in the vicinity of Sanliurfa. Journal of Animal and Veterinary Advances. 9: 292-296.
36- Shayan, P. and S. Rahbari. 2005. Simultaneous differentiation between Theileria spp. and Babesia spp. on stained blood smear using PCR. Parasitology Research 97: 281-286.
37- Simking, P., N. Yatbantoong, N. Saetiew, S. Saengow, W. Yodsri, R. Chaiyarat, S. Wongnarkpet and S. Jittapalapong. 2014. Prevalence and risk factors of Babesia infections in cattle trespassing natural forest areas in Salakpra Wildlife Sanctuary, Kanchanaburi Province. Journal of Tropical Medicine and Parasitology. 37: 10-19.
38- Terkawi, M. A., H. Alhasan, N. X. Huyen, A. Sabagh, K. Awier, S. Cao, Y.-K. Goo, G. Aboge, N. Yokoyama and Y. Nishikawa. 2012. Molecular and serological prevalence of Babesia bovis and Babesia bigemina in cattle from central region of Syria. Veterinary Parasitology. 187: 307-311.
39- Vahedi Nouri, N. and V. Noaman. 2020. The molecular study of babesia species in the cattles of Mazandaran province. Journal of Animal Biology. 12: 81-90.
40- Yusuf, J. J., P. Jimma and T. Ethiopia. 2017. Review on Bovine Babesiosis and its Economical Importance. J Vet Med Res. 4: 1090.
41- Ziapour, S. P., B. Esfandiari and M. R. Youssefi. 2011. Study of the prevalence of babesiosis in domesticated animals with suspected signs in Mazandaran province, north of Iran, during 2008. Journal of Animal and Veterinary Advances. 10: 712-714.