Validation of human urinary dipsticks for different biochemical parameters in dog

Document Type : Full Research Paper

Authors

1 Basic sciences,, School of Veterinary Medicine. Shahid Bahonar University of Kerman. Kerman. Iran

2 Department of Clinical Sciences, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

3 Department of Clinical Science, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract

Urinalysis provides the valuable information about physiological and pathological conditions of the patients. The aim of this study was to determine accuracy of human urinary dipsticks for assessment of different biochemical parameters such as pH, specific gravity, protein, glucose, bilirubin and leukocyte in canine urine samples. For this purpose, urine samples of 60 dogs were collected and paired measurements of the urinary parameters with both different commercial dipsticks (Kimia and MN) and standard tests were analyzed. The correlation coefficient was fair between the assayed urine protein with two urine strips and standard method (r=0.68). For glucose, correlation coefficient was good between reference method and the urine strips (r=0.78). In the case of protein and glucose, both strips showed excellent sensitivity and negative predictive value (NPV). Correlation coefficient was good between reference method and the urine strips (r=0.91). Although correlation was significant between urine specific gravity assayed using refractometer and strips (r=0.38), correlation coefficient was poor. It seemed that employing human urine strips was not suitable method for evaluating specific gravity and refractometer was preferred. In conclusion, it is suggested that human urine strips can be used to determine protein, glucose and pH in urine of dog.  

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References

1.Rizzi, T.E., Valenciano, A.C., Bowles, M., Cowell, R.L., Tyler, R. and D.B., DeNicola. 2017. Atlas of canine and feline urinalysis. John Wiley & Sons.
2.Yadav, S.N., Ahmed, N., Nath, A.J., Mahanta, D. and M.K., Kalita. 2020. Urinalysis in dog and cat: A review. Veterinary World 13: 2133-2141.
3.Grauer, G.F. 2007. Measurement, interpretation, and implications of proteinuria and albuminuria. Veterinary Clinics of North America: Small Animal Practice 37: 283-295.
4.Meindl, A.G., Lourenço, B.N., Coleman, A.E. and K.E., Creevy. 2019. Relationships among urinary protein to creatinine ratio, urine specific gravity, and bacteriuria in canine urine samples. Journal of Veterinary Internal Medicine 33: 192-199.
5.Aldridge, C.F., Behrend, E.N., Smith, J.R., Welles, E.G. and H.P., Lee. 2020. Accuracy of urine dipstick tests and urine glucose-to-creatinine ratios for assessment of glucosuria in dogs and cats. Journal of the American Veterinary Medical Association 257: 391-396.
6.Mie, K., Hayashi, A., Nishida, H., Okamoto, M., Yasuda, K., Nakata, M., Fukatsu, K., Matsunami, N., Yamashita, S., Ohashi, F. and H., Akiyoshi. 2019. Evaluation of the accuracy of urine analyzers in dogs and cats. Journal of Veterinary Medical Science 81: 1671-1675.
7.Defontis, M., Bauer, N., Failing, K. and A., Moritz. 2013. Automated and visual analysis of commercial urinary dipsticks in dogs, cats and cattle. Research in Veterinary Science 94: 440-445.
8.Reine, N.J. and C.E., Langston. 2005. Urinalysis interpretation: how to squeeze out the maximum information from a small sample. Clinical Techniques in Small Animal Practice 20: 2-10.
9.Mundt, L., and K., Shanahan. 2020. Graff's textbook of urinalysis and body fluids. 3rd ed. Jones & Bartlett Publishers.
10.Athanasiou, L.V., Katsoulos, P.D., Katsogiannou, E.G., Polizopoulou, Z.S., Diamantaki, M., Kamatsos, C. and G., Christodoulopoulos. 2018. Comparison between the urine dipstick and the pH meter to assess urine pH in sheep and dogs. Veterinary Clinical Pathology 47: 284-288.
11.Pressler, B.M., Vaden, S.L., Jensen, W.A. and D., Simpson. 2002. Detection of canine microalbuminuria using semiquantitative test strips designed for use with human urine. Veterinary Clinical Pathology 31: 56-60.
12.Reppas, G. and S.F., Foster. 2016. Practical urinalysis in the cat: 1: Urine macroscopic examination ‘tips and traps’. Journal of Feline Medicine and Surgery 18: 190-202.
13.Rizzi, T.E. 2014. Urinalysis in Companion Animal Part 1: Collection, Sample Handling, and Initial Evaluation. Today’s Veterinary Practice 4: 65-68.
14.Papasouliotis, K., Cue, S., Crawford, E., Pinches, M., Dumont, M. and K., Burley. 2006. Comparison of white blood cell differential percentages determined by the in_house LaserCyte hematology analyzer and a manual method. Veterinary Clinical Pathology 35: 295-302.
15.Parrah, J.D., Moulvi, B.A., Gazi, M.A., Makhdoomi, D.M., Athar, H., Din, M.U., Dar, S. and A.Q., Mir. 2013. Importance of urinalysis in veterinary practice–A review. Veterinary World 6: 640-646.
16.Kwong, T., Robinson, C., Spencer, D., Wiseman, O.J. and F.E.K., Frankl. 2013. Accuracy of urine pH testing in a regional metabolic renal clinic: is the dipstick accurate enough? Urolithiasis 41: 129-132.
17.Harley, L. and C., Langston. 2012. Proteinuria in dogs and cats. The Canadian Veterinary Journal 53: 631-638.
18.Kennedy, S.M., Lulich, J.P., Ritt, M.G. and E., Furrow. 2016. Comparison of body condition score and urinalysis variables between dogs with and without calcium oxalate uroliths. Journal of the American Veterinary Medical Association 249: 1274-1280.
19.Thornton, L.A., Burchell, R.K., Burton, S.E., Lopez Villalobos, N., Pereira, D., MacEwan, I., Fang, C., Hatmodjo, A.C., Nelson, M.A., Grinberg, A. and N., Velathanthiri. 2018. The effect of urine concentration and pH on the growth of Escherichia coli in canine urine in vitro. Journal of Veterinary Internal Medicine 32: 752-756.
20.Afsahi, A., Ahmadi-Hamedani, M. and M., Khodadi. 2020. Comparative evaluation of urinary dipstick and pH-meter for cattle urine pH measurement. Heliyon 6: e03316.
21.Hekmatynia, F., Eskandarzadeh, N., Imani, M., Rezaei, M. and M., Zamani-Ahmadmahmudi. 2019. The diagnostic performance of human urinary dipsticks to estimate urine pH, specific gravity (SpG), and protein in horses: are they reliable? BMC Veterinary Research 15: 1-5.
22.Zatelli, A., Paltrinieri, S., Nizi, F., Roura, X. and E., Zini. 2010. Evaluation of a urine dipstick test for confirmation or exclusion of proteinuria in dogs. American Journal of Veterinary Research 71: 235-240.
23.de Buys Roessingh, A.S., Drukker, A. and J.P., Guignard. 2001. Dipstick measurements of urine specific gravity are unreliable. Archives of Disease in Childhood. 85: 155-157.
24.Melandri, M., Veronesi, M.C. and S., Alonge. 2020. Urinalysis in Great Dane puppies from birth to 28 days of age. Animals 10: 636-649.
25.Ferreira, M.D.F., Garcia Arce, M., Handel, I.G., Breheny, C.R. and A.G., Gow. 2018. Urine dipstick precision with standard visual and automated methods within a small animal teaching hospital. Veterinary Record. 183: 415-415.

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History

  • Receive Date: 30 June 2021
  • Revise Date: 29 August 2021
  • Accept Date: 30 August 2021

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