Production of hydrolyzed protein from vermicompost worms Eisenia foetida for use in vaccine production process

Document Type : Full Research Paper

Authors

1 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

2 Research Institute of Applied Sciences (RIAS), ACECR, Tehran, Iran

Abstract

The most basic application of protein hydrolysis is nitrogen supply for bacterial culture media. Considering that about 200 million doses of Clostridium bacteria are used annually in our country and to produce this vaccine dose, 16 tons of hydrolyzed protein (peptone) is needed. Currently, this need is supplied through imports. Therefore, achieving the technical knowledge of peptone production from various inexpensive sources in the country is essential. Therefore, due to the development of vermicompost worm farms, inexpensiveness, availability, high protein in the body, hydrolysis protein of this animal was performed. Due to the development of vermicompost worm farms, inexpensiveness, availability, high protein in the body, protein hydrolysis of this animal was performed. Samples of worms were purchased from farms around Mashhad. For disposal of digestive system materials, worms were placed in a clean water and sand container for 24 hours. Then, by homogenizing them in water for 30 minutes, homogeneous suspension and uniformity of them were provided. Subsequently, chloroform was performed in two 30-minute times at 25c and 60c temperature, remove fat from protein tissue. Using six-normal hydrolytic hydrochloric acid vapor phase at 110c for 10 hours, protein hydrolysis was performed. Complete drying of the sample was completed in 3 hours under vacuum conditions with one bar pressure and 70°C. In this study, from the initial value of 10 gr of vermicomposting worms, three gr of hydrolyzed proteins were produced This amount of hydrolysis protein produced was 30% of the initial weight of worms.

Keywords

References

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History

  • Receive Date: 03 April 2021
  • Revise Date: 23 June 2021
  • Accept Date: 28 June 2021

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