Effect of spliceosome gene complex underlying the transition between the laying and brooding phases in pituitary transcriptome profile of breeder turkey

Document Type : Full Research Paper

Authors

1 Department of Animal Science, Animal Science and Food Technology Faculty, Agricultural Sciences and Natural Resources, University of Khuzestan, Mollasani, Iran.

2 Department of Biotechnology, Animal Science Research Institute of IRAN (ASIR), Agriculture Research, Education and Extension Organization (AREEO), Karaj, Iran.

3 Department of Animal Science, Agriculture Faculty, University of Buali Sina, Hamadan, Iran

Abstract

The spliceosome is a large ribonucleoprotein complex that guides pre-mRNA splicing in eukaryotic cells. In this research, pituitary transcriptome profile of commercial breeder turkey of BUT breed was studied during the laying and broodiness phases. A total of 334 differentially expressed genes during egg-laying phases and brooding phases were identified. There were 229 upregulated and 104 downregulated at egg-laying phases compared with brooding phases. In the present study results of both GO and KEGG analysis suggested that the genes in the spliceosome complex are critical in the transition from the egg-laying to brooding phase turkeys. In addition, in the calcium ion transport in the biological process, splicing activates and creates various isoforms of the calcium/calmodulin alpha, which plays an important role in the neurotransmitter and hormonal systems of turkeys. In this study it was revealed that SNRPD1, SNRPF and SNRPA1/U2A in spliceosome complex were essential for folliculogenesis and fertility. HSPA8 was
significantly down-regulated at laying turkeys compared with broodiness turkeys was involved in autophagy and follicular regression in broody turkey.
    

Keywords

References

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History

  • Receive Date: 30 January 2021
  • Revise Date: 25 March 2021
  • Accept Date: 04 April 2021

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