Molecular Detection of Haemoproteous Parasite in Wild life Partridges in Mazandaran Province

Document Type : Full Research Paper

Authors

1 Department of Cellular and Molecular, Babol Branch, Islamic Azad University, Babol, Iran.

2 Department of Parasitology, Islamic Azad University, Babol Branch, Babol, Iran

3 Faculty of Veterinary Medicine, Babol-Branch, Islamic Azad University, Babol, Iran.

Abstract

Haemoproteus. spp., infactly is a protozoan parasite. Over than 140 species of that have been known that generally it is reported among wild birds and other birds categories. Its species vector is Hippoboscidae fly and colicoidae mosquito. In birds who involved in weakness of immune system, Haemoproteus. spp. may result in Hemolytic anemia, anorexia and death.  The aim of this study, Molecular study on Haemoproteus. spp. parasite in wildlife Partridge of Mazandaran province and identifying new species of parasite. First, 12 Pieces of wild partridges were sampled with trapping in nature. Then smears of colored bloods by giemsa studied to identify macro- and micro-gametes of Haemoproteus spp. in birds red blood cells, then positive samples, were studied in order to perform PCR method, on cytochrome b like gene. Morphology study in this research demonstrate 5 positive of 12 samples. Infection of Haemoproteus spp. in Positive samples were confirmed by study with PCR method and investigating sample nucleotide sequence using “BLAST” software. Comparing sample nucleotide sequence with nucleotide sequence of other species of Haemoproteus spp. parasite showed that similarity between sample nucleotide sequences with mentioned nucleotide sequences is low. Data analysis showed that identified species of Haemoproteous parasite in this study is a new species called “Haemoproteus. partridage”.

Keywords

References

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History

  • Receive Date: 06 December 2020
  • Revise Date: 08 March 2021
  • Accept Date: 15 March 2021

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