Antifungal activity of Amphotericin B-Loaded N, N, N-Trimethyl Chitosan Nanoparticles against Candida albicans

Document Type : Full Research Paper

Authors

1 Department pathobiology faculty of veterinary specialized science. science and research branch.islamic azad university,tehran,iran

2 Department of Pathobiology, Faculty of Veterinary Specialized Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Department of Human Vaccine and Serum, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

4 Department of Tuberculosis, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

Abstract

Recent advancements in drug delivery system through development of nanomedicine has significantly improved the delivery of the medicine to the target tissue, consequently reduced the required therapeutic dose, toxicity and side effects. The aim of this study was test antifungal susceptibility of trimethylchitosan nanoparticles in combination with amphotericin B (NPs-AmB) through broth micro-dilution method described in CLSA-A3 guidelines on resistant (R) and susceptible (S) Candida albicans isolates and the cell viability and proliferation assays. Minimum inhibitory concentrations (MICs) 90 % of NPs-AmB and AmB determined to be 0.25 and 0.75 µg/mL, and minimum fungicidal concentration (MFC) were 0.5 and 1 µg/mL for S-isolate. MIC 90 for R-isolate were 8 and 32 µg/mL and MFC were 32 and 64 µg/mL respectively demonstrating a significant improvement in antifungal activity (p

Keywords

References

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History

  • Receive Date: 18 November 2020
  • Revise Date: 24 January 2021
  • Accept Date: 06 February 2021

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