Investigation of the prevalence of virulence and antibiotic resistance genes in Escherichia coli isolated from ostriches and human urinary tract infections

Document Type : Full Research Paper

Authors

1 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran.

2 Department of clinical sciences, Faculty of veterinary medicine, Ferdowsi university of Mashahd, Mashhad, Iran.

Abstract

Escherichia coli is an important member of the natural gut flora of humans, warm-blooded animals and birds. Often, infections caused by Escherichia coli occur opportunistically, and the main and most common infections are uropathogenic Escherichia coli (UPEC) and pathogenic Escherichia coli in birds (APEC). In the present study, 30 extra-intestinal Escherichia coli samples were collected from 30 ostriches suspected of having septicemia and 30 plates containing Escherichia coli from patients with urinary tract infections. The isolates were studied for 8 common virulence genes (astA, iss, irp2, papC, iucD, tsh, vat, cva A / B cvi cvaC, as well as 6 antibiotic resistance genes (tetA, blaTEM, sul1, aadA1, aaC. (3) -IV, OnRA) were examined. The results of this study on the prevalence and frequency of virulence factors indicated that the highest frequency in APEC strains was related to papC genes (16/66%) and iss (20%). In UPEC strains, it was related to irp2 (53.33%) and cva A/B cvi cvaC(40%) genes. Also, in the study of antibiotic resistance in APEC isolates, the highest frequency was related to aac (3) -IV gene (30%) and the highest resistance was to the aadA1 gene (8.4%). Despite the genetic differences in some virulence factors between the two groups of bacteria in the present study, our results showed that major genetic similarities Bacterial isolates from humans and ostriches, and further studies on the importance of the prevalence of these viruses and resistance genes in the epidemiology and pathogenesis of bacteria in the human and ostrich populations.

Keywords

References

1. Amiri, M., Jajarmi, M., Ghanbarpour, R. 2017. Prevalence of resistance to quinolone and fluoroquinolone antibiotics and screening of qnr genes among Escherichia coli isolates from urinary tract infection. Int J Enteric Pathog 5:100-105. 
2. Amiri, M., Farzin, H., Jamshidian-Mojaver, M. 2019. Phenotypic and Genotypic Study of Antibiotic Resistance among Escherichia coli Isolates from Human Urinary Infection Cases in Bojnord Province. AJCM 26: 173-180.
3. Amani, F., Hashemitabar, G., Ghaniei, A. et al. 2020. Antimicrobial resistance and virulence genes in the Escherichia coli isolates obtained from ostrich. Trop Anim Health Prod. 52: 3501–3508 
4. Askari, N., Ghanbarpour, R. 2019. Molecular investigation of the colicinogenic Escherichia coli strains that are capable of inhibiting E. coli O157: H7 in vitro. BMC 15: 14-18.
5. Bekal, S., Lin, A., Vincent, A., Berry, C., Gilmour, M., Fournier, É Tremblay, C. 2015. Draft genome sequence of a necrotoxigenic Escherichia coli isolate. Genome announcements. ASM 3: 250-258.
6. Escobar‐Páramo, P., Le Menac’h, A., Le Gall, T., Amorin, C., Gouriou, S., Picard, B., Denamur, E. 2006. Identification of forces shaping the commensal Escherichia coli genetic structure by comparing animal and human isolates. Environmental microbiology 8:1975-1984.  
7. Ewers, C., Li, G., Wilking, H., Kieβling, S., Alt, K., Antáo, E. M., Böhnke, U. 2007. Avian pathogenic, uropathogenic, and newborn meningitis-causing Escherichia coli: how closely related are they. IJMM 297: 163-176. 
8. Girardeau, J. P., Dalmasso, A., Bertin, Y., Ducrot, C., Bord, S., Livrelli, V., Martin, C. 2005. Association of virulence genotype with phylogenetic background in comparison to different seropathotypes of Shiga toxin-producing Escherichia coli isolates. JCM 43:6098-6107.
9. Hojati, Z., Zamanzad, B., Hashemzadeh, M., Molaie, R., Gholipour, A. 2015. The FimH gene in uropathogenic Escherichia coli strains isolated from patients with urinary tract infection. JJM 8: 180-186.
10. Jajarmi, M., Ghanbarpour, R., Sharifi, H., Golchin, M. 2015. Distribution pattern of EcoR phylogenetic groups among shiga toxin-producing and enteropathogenic Escherichia coli isolated from healthy goats. Int J Enteric Pathog 3: 1-27971. 
11. Johnson, J. R., Delavari, P., Kuskowski, M., Stell, A. L. 2001. Phylogenetic distribution of extraintestinal virulence-associated traits in Escherichia coli. IJID 183: 78-88.
12. Jamshidian-Mojaver M, Amiri M, Farzin H. 2020. Phenotypic and genotypic evaluation of fluoroquinolones resistance in Klebsiella pneumoniae isolates from urinary tract infections in Bojnourd city. MJMS 63: 2335-40
13. Kader, A. A., Kumar, A., Kamath, K. A. 2007. Fecal carriage of extended-spectrum β-lactamase–producing Escherichia coli and Klebsiella pneumoniae in patients and asymptomatic healthy individuals. ICHE 28: 1114-1116.
14) Mashayekhi F, Moghny M, Faramarzpoor M, Yahaghi E, Khodaverdi Darian E, Tarhriz V, Dormanesh B. 2014. Molecular characterization and antimicrobial resistance of uropathogenic Escherichia coli. Iranian Journal of Biotechnology 12: 32-40.
15. Micenková, L., Bosák, J., Štaudová, B., Kohoutová, D., Čejková, D., Woznicová, V. ,Šmajs, D. 2016. Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates. Microbiology Open 5: 490-498.
16. Munkhdelger, Y., Gunregjav, N., Dorjpurev, A., Juniichiro, N., Sarantuya, J. 2017. Detection of virulence genes, phylogenetic group and antibiotic resistance of uropathogenic Escherichia coli in Mongolia. JIDC 11: 51-57.
17. Naderi, Z., Ghanbarpour, R., Sami, M. 2016. Antimicrobial resistance characteristics and phylogenetic groups of Escherichia coli isolated from diarrheic calves in southeast of Iran. Int J Enteric Pathog 4: 1-7. 
18. Najafi, S., Rahimi, M., Nikousefat, Z. 2019. Extra-intestinal pathogenic Escherichia coli from human and avian origin: Detection of the most common virulence-encoding genes. VRF 10: 43-48. 
19. Nateghi, F., Jafarpour, M., Nazemi, A. 2010. A Survey for Detection of Eight Correlated Genes of Avian Pathogenic Escherichia coli in Human Uropathogenic Escherichia coli. Journal of Microbial World 3:169-176.
20. Nisa, S., Scanlon, K. M., Donnenberg, M. S. 2013. Enteropathogenic Escherichia coli. In Escherichia coli , Academic Pres 5: 75-119.
21. Obeng AS, Rickard H, Ndi O, Sexton M, Barton M. 2012. Antibiotic resistance, phylogenetic grouping and virulence potential of Escherichia coli isolated from the faeces of intensively farmed and free range poultry. Veterinary microbiology 15:300-306.
22. Rodriguez-Siek, K. E., Giddings, C. W., Doetkott, C., Johnson, T. J., Fakhr, M. K., Nolan, L. K. 2005. Comparison of Escherichia coli isolates implicated in human urinary tract infection and avian colibacillosis. Journal of General Microbiology 151:2097-2110.
23. Shookohi, M., Rashki, A. 2016. Prevalence of toxigenic genes in Escherichia Coli isolates from hospitalized patients in Zabol, Iran. Int J Enteric Pathog 4: 1-5. 
24. Sun, H., Liu, P., Nolan, L. K., Lamont, S. J. 2015. Avian pathogenic Escherichia coli (APEC) infection alters bone marrow transcriptome in chickens. BMC genomics 16: 690-698. 
 

Files

History

  • Receive Date: 04 November 2020
  • Revise Date: 09 January 2021
  • Accept Date: 20 January 2021

Share

How to cite

Statistics