Comparison of microscopic and molecular methods in the diagnosis of Anaplasma marginale and determination of related risk factors in cattle in Semnan province

Document Type : Full Research Paper

Authors

1 Department of Parasitic Disease Research, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

2 Department of pathobiology, Faculty of veterinary medicine, Karaj branch, Islamic Azad University, Karaj, Iran

Abstract

This study aimed to determine the molecular prevalence of Anaplasma marginale among cattle of Semnan province. A total of 208 blood samples were randomly collected via the ear vein and jugular vein from healthy cattle for microscopy and molecular examination, respectively. The extracted DNA from blood cells was amplified by a pair of primer, which amplify an approximately 866bp DNA fragment from the region of the msp4 gene of  A. marginale and 45.2% of cattle blood samples were identified positive for A. marginale. Chi-square tests were used to compare molecular prevalence values relative to climate, altitude, longitude, latitude, season, farm-type, hygiene of the farm, vectors, farm density, age, sex, and milk yield. The significant major risk factors of A. marginale in cattle were identified as latitude, farm-type, vectors, age, and milk yield by the multivariable logistic regression analysis (P< 0.05). The examination of 50 microscopic fields showed 31.9% sensitivity and 54.4% specificity compared to molecular examination. The Kappa coefficient between molecular and microscopy (50 fields) techniques indicated a slight level of agreement (Kappa= 0.13). This study is the first molecular detection of A. marginale from cattle in Semnan province, Iran. Further researches are needed to determine the vectors, vector-host interactions, and genotypic variants that may affect the presence and distribution of Anaplasma species in Semnan province.

Keywords

References

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Veterinary Research & Biological Products
Volume 34, Issue 4 - Serial Number 4
January 2022
Pages 136-146

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History

  • Receive Date: 19 September 2020
  • Revise Date: 04 October 2020
  • Accept Date: 31 October 2020

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