Antagonistic Activity of Lactobacilli Isolated from Buffalo Milk and Yogurt against Some Food Pathogens in Golestan province

Document Type : Full Research Paper

Authors

1 Department of Microbiology, Gorgan Branch, Islamic Azad University, Gorgan, Iran

2 Department of Microbiology, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran

Abstract

Lactic acid bacteria which are found in large amounts in dairy products, have antagonistic activity against many pathogenic microorganisms. In this study, the inhibitory effect of Lactobacillus strains isolated from buffalo milk and yogurt in Bandar Gaz city was investigated. Raw milk and yogurt samples were cultivated on MRS medium, incubating anaerobically at 37°C for 48 h. The suspected colonies were identified on the basis of Gram’s staining and biochemical tests. The antibacterial activity of the cell-free supernatant extracted from Lactobacillus strains was determined using the agar well diffusion method against standard strains including Shigella flexneri ATCC 12022 ، Salmonella typhimurium ATCC 14028 ، Vibrio paraheamolyticus ATCC 17802 ، Escherichia coli ATCC 11303 and Staphylococcus aureus ATCC 29213. A total 10 Lactobacillus strains were identified as L. plantarum, L. casei, L. acidophilus. The cell-free supernatant of L. plantarum A1 had a relatively strong inhibitory effect against S. aureus and a moderate inhibitory effect against E. coli and S. flexneri, while no inhibitory effect on V. paraheamolyticus and S. typhimurium growth. L. casei B1 showed a moderate inhibitory effect against S. flexneri and S. aureus. The supernatant of L. acidophilus R1 vs. S. aureus showed a moderate inhibitory effect and had an inhibitory effect on V. paraheamolyticus. The results of this study showed that Lactobacilli isolates had good inhibitory activity against some food pathogens, therefore further studies on their use as biopreservatives to prevent the survival and proliferation of microorganisms in food products are recommended. 

Keywords


1-Chowdhury, A., Md.N. Hossain, NJ. Mostazir, Md. Fakruddin, M. Billah, et al.2012. Screening of Lactobacillus spp. from Buffalo Yoghurt for Probiotic and Antibacterial Activity. Journal of Bacteriology and Parasitology 3: 3-8.
2-Davoodabadi, A., MM. Soltan Dallal, E. Lashani, M. Tajabadi Ebrahimi. 2015. Antimicrobial Activity of Lactobacillus spp. Isolated From Fecal Flora of Healthy Breast-Fed Infants against Diarrheagenic Escherichia coli. Jundishapur Journal of Microbiology 8(12):1-6.
3-Dorri, K., N. Namdar, V. Hemayatkhah Jahromi. 2013. Isolation of Lactobacilli from Dairy Products and Their Effects on the Main Pathogenic Bacteria in Stomach and Intestine. Medical Laboratory Journal 7: 21-29.
4-Eid, R., J. El Jakee, A. Rashidy, H. Asfour, S. Omara, MM. Kandil, Z. Mahmood, J. Hahne, AA.  Seida. 2016. Potential Antimicrobial Activities of Probiotic Lactobacillus Strains Isolated from Raw Milk. Journal of Probiotics & Health 4: 1-8.
5-Farahbakhsh, M., H. Hakimi, R. Bahram Abadi. 2013. Survey on Isolation of Probiotic Lactobacilli from Traditional Yogurts Produced in Rural Areas of Rafsanjan and their Antimicrobial Effects. Journal of Rafsanjan University of Medical Sciences 12: 733-46. 
6-Forhad, M. H., S.M. Khaledur Rahman, Md. Shahedur Rahman, F. Karim Saikot, K. Chandra Biswas. 2015. Probiotic Properties Analysis of Isolated Lactic Acid Bacteria from Buffalo Milk. Archives of Clinical Microbiology 7: 1-6.
7-Fozouni, L., M. Yaghoobpour, A. Ahani Azari. 2019. Probiotics in Goat Milk: A Promising Solution for Management of Drug-Resistant Acinetobacter baumannii. Jorjani Biomedicine Journal 7: 31-38.
8-Gad, S.A., R.M.A. El-Baky, A.B. F. Ahmed, G.F.M. 2016. Gad. In vitro evaluation of probiotic potential of five lactic acid bacteria and their antimicrobial activity against some enteric and food-borne pathogens. African Journal of Microbiology Research 10: 400-409.
9-González, L., H. Sandoval, N. Sacristán, JM. Castro, JM. Fresno, ME. Tornadijo. 2007. Identification of lactic acid bacteria isolated from Genestoso cheese throughout ripening and study of their antimicrobial activity. Food Control 18(6):716-22. 
10-Hossein Alipour, E., K. Mardani, M. Moradi. 2018. Isolation and Identification of Lactobacillus salivarius from Buffalo Milk and Evaluation of Its Antimicrobial Activity. Iran. Iranian Journal of Medical Microbiology 12: 96-106.
11-Iranmanesh, M., H. Ezzatpanah, N. Mojgani, MA. Karimi Torshizi, M. Aminafshar, M. Maohamadi. 2012. Isolation of Lactic Acid Bacteria from Ewe Milk, Traditional Yoghurt and Sour Buttermilk in Iran. European Journal of Food Research & Review 2: 79-92.
12-Ivanova, I., P. Kabadjova, A. Pantev, S. Danova, X. Dousset. 2000. Detection, purification and partial characterization of a novel bacteriocin substance produced by Lactococcus lactis subsp. lactis b14 isolated from Boza- Bulgarian traditional cereal beverage. Biocatalysis 41: 47-53.
13-Juárez Tomás, MS., CI. Saralegui Duhart, PR. De Gregorio, E. Vera Pingitore, ME. Nader-Macías. 2011. Urogenital pathogen inhibition and compatibility between vaginal Lactobacillus strains to be considered as probiotic candidates. European Journal of Obstetrics & Gynecology and Reproductive Biology 159(2):399-406.
14-Karami, S., M. Roayaei, H. Hamzavi, M. Bahmani, H. Hassanzad-Azar, L. Mahmoodnia, M. Rafieian-Kopaei. 2017. Isolation and identification of probiotic Lactobacillus from local dairy and evaluating their antagonistic effect on pathogens. International Journal of Pharmaceutical Investigation 7: 137–141.
15-Kasra-Kermanshahi, R. and Mobarak-Qamsari, E. 2015. Inhibition Effect of Lactic Acid Bacteria against Food Born Pathogen, Listeria monocytogenes. Applied Food Biotechnology 2: 11-19.
16-Kazemi Darsnaki, R., N. Ghaemi, M. Mirpour. 2010. Antimicrobial activity of lactic acid bacteria isolated from probiotic products (Lactobacillus and Bifidobacterium). Journal of Microbial Biotechnology Islamic Azad University 2: 29-36. 
17-Kiai, A., N. Amir Mozaffari, H. Sami Al-Adab, N. Jandaghi, A. Ghaemi. 2006. Antagonistic effect of lactic acid bacteria isolated from yogurt against pathogenic bacteria. Journal of Gorgan University of Medical Sciences 8(1): 33 - 28. 
18-Koohsari, H., Z. Rashti, S. Arab. 2019. The Isolation of lactic acid bacteria from local dairy products of Gorgan town with the ability to inhibit the growth of some gastrointestinal pathogens. Journal of Food Microbiology 6: 22 – 36.
19-Manzoor, A., I. Ul-Haq, SH. Baig, J. Iqbal Qazi, S. Seratlic. 2016. Efficacy of Locally Isolated Lactic Acid Bacteria against Antibiotic-Resistant Uropathogens. Jundishapur Journal of Microbiology 9: 1-11.
20-Naeemi, Z., H. Koohsari, H.R. Pordeli. 2019. Isolation of lactic acid bacteria with probiotic potential from bovine colostrum in livestock farms of Ramian Township in located in the north of Iran. International Journal of Molecular and Clinical Microbiology 9: 1097-1107.
21-Narimani, T., A. Tari Nejad. 2014. Isolation and biochemical and molecular identification of probiotic bacteria from traditional buffalo milk and yogurt of Khoy city. Journal of Food Research 42 (3): 349-335. 
22-Rahimpour Hesari, M., R. Kazemi Darsanaki, A. Salehzadeh. 2017. Antagonistic Activity of Probiotic Bacteria Isolated from Traditional Dairy Products against E. coli O157:H7. Journal of Medical Bacteriology 6: 23-30.
23-Saud, B., P. Pandey, G. Paudel, G. Dhungana G, V. Shrestha. 2020. In-vitro Antibacterial Activity of Probiotic against Human Multidrug Resistant Pathogens. Archives of Veterinary Science and Medicine 3: 31-39.
24-Sikarchi, A., L. Fozouni. 2018. Inhibitory Effect of Probiotic Bacteria Isolated from Camel Milk on Clinical Strains of Drug-Resistant Helicobacter pylori. Medical Laboratory Journal 12: 20-26.
25-Servin, AL. 2004. Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. FEMS Microbiology Reviews 28(4):405-40. 
26-Slozilova, I., S. Purkrtova, M. Kosova, M. Mihulova, E. Svirakova, K. Demnerova. 2014. Antilisterialactivity of lactic acid bacteria against Listeria monocytogenes strains originating from different sources. Czech Journal of Food Sciences 32: 145-151.
27-Taheri, P., N. Samadi, M.R. Khoshayand, M. Fazeli, H. Jamalifar, M.R. Ehsani. 2011. Study on the Antibacterial Activity of Lactic Acid Bacteria Isolated from Traditional Iranian Milk Samples. International Journal of Agricultural Science and Research 2: 27-34.
28-Tesfaye, A. 2014. Antagonism and primary in vitro probiotic evaluation of lactic acid bacteria recovered from Ergo. Journal of Agricultural and Biological Science 7: 240-245.
29-Zare Mirzaei E, E. Lashani, A. Davoodabadi. 2018. Antimicrobial properties of lactic acid bacteria isolated from traditional yogurt and milk against Shigella strains. GMS Hygiene and Infection Control 13: 1-5.