Effect of cysteamine antioxidant on cellular parameters and frozen sperm quality in ram

Document Type : Full Research Paper

Authors

1 Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

3 Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

Abstract

The aim of this study was improvement of ram frozen semen efficiency using addition of cysteamine antioxidant to freezing medium. In this experiment, semen samples were collected from five Zandi rams and add to the extender containing 0, 1, 2, 4 and 8 mM cysteamine. In sperm quality evaluation, sperm samples were evaluated and motion parameters, membrane integrity, morphology, acrosome integrity, mitochondria activity, viability and lipid peroxidation were assessed. The results showed using 2 mM cysteamine resulted in improving of sperm total and progressive motility, membrane integrity, mitochondria activity, acrosome integrity, and viability as well as reduction of apoptosis rate and lipid peroxidation. Using the treatment of 8 mM cysteamine caused to reduction of sperm quality after thawing and the antioxidant did not have any effect on sperm morphology. In conclusion, using 2 mM cysteamine in freezing extender could be a helpful strategy for improvement of ram sperm quality after cryopreservation.

Keywords

References

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History

  • Receive Date: 06 April 2020
  • Revise Date: 08 September 2020
  • Accept Date: 10 September 2020

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