Identification and phylogenetic analysis based on Newcastle disease virus gene F isolated from broiler poultry farms in Markazi province

Document Type : Full Research Paper

Authors

1 Agricultural Research Education and Extension Organization, Razi Vaccine and Serum Research Institute, Arak Branch , Iran

2 Agricultural Research Education and Extension Organization, Razi Vaccine and Serum Research Institute, Karaj, Iran

Abstract

Newcastle Disease Virus (NDV)is one of the most contagious viral diseases of poultry with rapid spread around the world. It identified by respiratory symptoms, nervous and digestive disorders, and high mortality. NDv causes significant economic losses in poultry industry throughout the world. Avian paramixovirus serotype 1(APMV-1) is a member of family paramyxoviridae, the genus Avulavirus, Newcastle disease virus belongs to APMV-1. The aim of this study to recognize and phylogenic analysis of Newcastle disease Virus isolated from poultry farms in Markazi Province by RT-PCR. In this research, recognition and phylogenic analysis was performed based on F gene of Newcastle disease virus. About 30 samples including brain, trachea, lung and spleen were obtained from suspected farms. Then, They were inoculated to 9 - 11 days age SPF embryonic eggs. After that 2-5 days, The Allantoic fluid harvested, the HA test was performed and genetically analysis using specific primer for F gene was done by RT PCR. In this study, Among the samples 13 of them was confirmed as Newcastle disease virus. Phylogeny tree was constructed for F gene using MEGA 6 software. The results showed that all isolates NDV belonged to genotype VII and subgenotype VII-j. These obtained data can use to improve the efficacy of the vaccine and disease prevention program, as well as its use for vaccine production against the Newcastle-disease causative agents.

Keywords

References

1- Afzal, F., Saliha, U. (2015). Isolation characterization of Newcastle disease viruse & comparitive efficacy of different vaccine regimes in broiler birds. The journal of animal & plant sciences, 25(4): page:971-976.
2-Atyabi N., Alemohammad H. (2017) . Serological valuation of Newcastle disease vaccination titer in the broiler farms of Mazandaran province during 2014-2017; veterinary Researches & Biological products NO 118, pp:58-64.
3-Bogoyavlenskiy, A., Berezin, V., Prilipov, A., Usachev, E., Lyapina, O. (2009). Newcastle disease outbreaks in Kazakhstan and Kyrgyzstan during 1998, 2000, 2001, 2003, 2004, and 2005 were caused by viruses of the genotypes VIIb and VIId. Virus Genes, 39: 94-101.
4- Boroomand,M., Jafari, R.A., Mayahi, M.(2016). Molecular characterization and phylogenetic study of the fusion genes of Newcastle disease virus from the recent outbreaks in Ahvaz, Iran, VirusDis. (January–March 2016) 27(1):102–105.
5- Diel, D.G., Dasilva, L.H., Liu, H., Wang, Z., Miller, P.J., Afonso, C.L. (2012). Genetic diversity of avianparamyxovirus type1: proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes. Infection, Genetics and Evolution, 12: 1770–1779.
6- Ebrahimi, M. M., Shahsavandi, S.( (2012) ). Phylogeny and evolution of Newcastle disease virus genotypes isolated in asia during 2008-2011; Virus genes 45: 63-68.
7- Esmaelizad,M., Mayahi ,V., Pashaei,M., Goudarzi, H.( 2016). Identiļ¬cation of novel Newcastle disease virus sub-genotype VII-(j) based on the fusion protein. Arch Virol, DOI 10.1007/s00705-016-3189-9.
8- Farooq, M., Saliha, U., (2014). Biological & genotypic characterization of Newcastle disease virus isolated from disease outbreaks in commercial poultry farms in northern Punjab, Pakistan. Virology reports 3, 30-39.
9- Ganar, K., Das, M., . (2014). Newcastle disease virus: Current status and our understanding; viruse research 184,71-81.
10-Ghalyanchi,A.L., Hosseini, H., karimi, V.(2012). phylogenetic study base on matrix gene of Iranian Newcastle disease virus isolates, 2011-2012;comp Clin pathol .
11- Ghalyanchi, L.A., Hosseini, H., Karimi, V.(2014). Phylogenetic study based on the phosphoprotein gene of Iranian Newcastle disease viruses (NDV) isolates, 2010- 2012; Iranian Journal of veterinary medicine, 8(2):73-77.
12-Giovanni, C., Susta, L.,. (2011) . Newcastle disease: a review of field recognition & current methods of laboratory detection. Journal of Veretinary Diagnostic Investigation 23(4)637-656.
13-Gould, A.R., Kattenbelt, J.A., Selleck, P., Hansson, E., Della-Porta, P.A., Westbury, H.A. (2001). Virulent Newcastle in Australia: Molecular epidemiological analysis of viruses isolated prior to and during the outbreaks of 1998-2000. Virus Research, 77: 51-60.
14-Hossenii, H., Ghalyanchi, A. L, Torai, R. ( 2014). Molecular characterization and phylogenetic study of Newcastle disease Viruses isolated in Iran, 2010-2012; Avian disease 58: 373-376.
15-Khan, S.T., Rehmani, Rue, C., Miller, P., Afonso, C.L.( 2010). Phylogenetic and pathological characterization of Newcastle disease virus isolates from Pakistan. Journal of Clinical Microbiology 48: 1892-1894.
16-Kianizadeh, M., Aini, I., Omar, A.R., Yusoff, K., Sahrabadi, M., Kargar, R. (2002). Sequence and phylogenetic analysis of the fusion protein cleavage site of Newcastle disease virus field isolates from Iran. Acta Virologica 46: 247-251.
17- Kianizadeh, M.,, lderis , A., Shahrabadi, M.S., Kargar, R. (1999). Biological and Molecular Characterization of Newcastle Disease Virus Isolated from Iran; Arch.RaZ; lns. 50.
18-Kiril, M. D., Claudio, L. A.(2016). Newcastle disease vaccines_ A solved problem or a continuous challenge? Veterinary Microbiology 7477 No pages 11.
19- Maclachlan, N. J., Dubove, E. J.( 2011). Book,Fenner's veterinary Virology, 4th edition,,chapter 17 p.p310-315.
20-Mayahi, M. Boroomand, Z . (2016) . Study on experimental infection of Newcastle disease virus in local breeder chicks infected with infectious bursal disease virus; veterinary Researches & Biological products NO 117, pp:11-23.
21- Mayahi, V., Esmaelizad, M.(2017). Molecular evolution and epidemiological links study of Newcastle disease virus isolates from 1995 to 2016 in Iran. Arch Virol 162:3727–3743.
22- Merabanpour, M., khoobyar, S., Rahimian, A. ( 2014). Phylogenetic characterization of the fusions genes of the Newcastle disease viruses isolated in Fars province poultry farms during 2009-2011;Veterinary research fourm; 5(3)187-191.
23- Miller, P. J., Kin.g, D. J. (2007). Antigenic difference among Newcastle disease virus strains of defferent genotyping used in vaccine formulation affect viral shedding after a virulent challenge .Vaccine 25, 7238-7246.
24-Miller, P.J., Kim, L.M., Ip. H.S., Afonso, C.L. (2009). Evolutionary dynamics of Newcastle disease virus. Virology, 391: 64-72.
25- Newcastle disease; The center for food security & public heath.( 2016). www.cfsph.iastate.edu.
26-OIE Terrestrial Manual 2012,C H A P T E R 2. 3 . 1 4.NEWCASTLE DISEASE.
27- Panda, A., Huang, Z., Elankumaran ,S. (2004). Role of fusion protein cleavage site in the virulence of Newcastle disease virus, Microbial Pathogenesis 36,1-10.
28-Samadi, S., Kianizadeh, M., Najafi, M.F., Nasab, S.D., Davatgar, A.M., Royaee, A., Pilvar, P. (2014). Molecular characterization and phylogenetic study of velogenic Newcastle disease virus isolates in Iran. Virus Genes, 48: 290-295.
29-Siddique, N., Naeem, K., (2013). sequence phylogenetic analysis of virulent Newcastle disease virus isolates from Pakistan during 2009-2013 reveals circulation of new sub genotype; virology 444,37-40.
30- Singh, K., Jindal, N., Gupta, S.L.( 2005). Detection of Newcastle disease virus genome from the field outbreaks in poultry By Reverse Transcription- polymerase chain reaction; International Journal of poultry Science 4(7)472-475.
31-Solomon, P., Abolnik, C., Joannis, T.M., Bisschop, S. (2012). Virulent Newcastle disease virus in Nigeria: identification of a new clade of sublineage 5f from livebird markets. Virus Genes, 44: 98–103.
32- Uddin H., Islam K., Barua M., Islam S. and Ahad A. (2017) Characterization of hemagglutination activity of emerging Newcastle disease virus in Bangladesh. International Journal of One Health. EISSN: 2455-8931, Open Access.
33-Van Borm, S., Obishakin, E., Joannis, T. (2012). Further evidence for the widespread co-circulation of lineages 4b and 7 velogenic Newcastle disease viruses in rural Nigeria. Avian Pathology, 41: 377–382.
34- Wang, Y., Zang, H., Gu, C. ( 2018). phylogenetic and pathotypic characterization of a Newcastle disease virus strain isolated from Ducks and pigeons in Hubei, China; Brazilian journal of poultry science , v.20/n.1/145-152.
35- Xu, Q., Sun,J. and et al; Genetic antigenic, and pathogenic characteristics of Newcastle disease viruses isolated from geese in china. Journal of veterinary diagnostic investigation 1-10.
36- Yune, N., Abdela, N.( 2017). update on epidemiology, Diagnosis & control technique of Newcastle disease, Journal of veterinary science & technology,8:2.
37-Setareh Khoobyar, Mohammad Bagher Nazari, Abdollah Rahimian, Mohammad Javad Mehrabanpour(2011) Isolation and pathotyping of Newcastle viruses by RT-PCR and MDT methods from broiler chickens in Fars province, Journal of Microbial WorldVolume 4, No. 1 & 2.
38-FATHI E.A., POURBAKHSH S.A., JAFARIAN MOHSEN(2009) PHYLOGENIE ANALYSIS AND MOLECULAR IDENTIFICATION OF NEWCASTLE DISEASE VIRUS IN FOWLS FORM IN CHAHARMAHAL-BAKHTIYARI PROVINCE, SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL , Volume 5 , Number 2 (23); Page(s) 50 To 55.
39-Statistics of Iran Statistics Center 1394, Management and Planning Organization, statistics of broiler farming statistics,page 22-28

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History

  • Receive Date: 11 January 2019
  • Revise Date: 06 February 2020
  • Accept Date: 18 April 2020

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