The effect of Ceratonia siliqua aqueous extract on canine semen quality on during storage at Refrigerator

Document Type : Full Research Paper

Authors

1 Department of Theirogenology, Faculty of Veterinary Medicine, Urmia University, Iran

2 Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azarbaijan, Iran

Abstract

Cooling and thawing result in oxidative damage, physical and chemical changes in the sperm. The aim of the present study was to evaluate the effect of hydroalcoholic extract of Ceratonia siliqua (carob) on semen quality during storage at 4 ° C. In this study, three male dogs aged 3-7 years were used for semen collection. The method of collection semen is manual. In this study, the second part of the ejaculate, which is rich in sperm, was used. Samples above 200 × 106 were used for the experiments. Sampling was performed twice a week, and to eliminate the diversity of sperm samples, all ejaculate dogs were pooled each time. The pooled sperm was centrifuged by centrifugation at 700 g for 5 minutes and the seminal plasma was discarded. After dilution, spermatozoa were divided into 5 groups: 1) Control, 2) control Sham, 3) 10 µg/ml of carob extract, 4) 20 µg/ml of carob extract, 5) 40 µg/ml of carob extract.
Hydroalcoholic extract of carob had the highest effect on sperm parameters improvement in the 20 µg/ml group compared to the other treatment groups and also had a significant difference with the control and control sham groups (P <0.05). This study demonstrates that Ceratonia siliqua hydroalcoholic extract, due to its antioxidant properties, can protect sperm against oxidative damage caused by refrigerated storage.

Keywords

References

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History

  • Receive Date: 02 March 2020
  • Revise Date: 05 April 2020
  • Accept Date: 08 April 2020

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