Production of Specific Immunoglobulin Y in Egg Yolk Against Some Calf Diarrhea Pathogens

Document Type : Full Research Paper

Authors

1 Animal Breeding and Genetics, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 Animal Breeding and Genetics, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

Calf diarrhea is a commonly reported disease and a major problem to the livestock industry. Although, antibiotics are used to treat diarrhea in calves, they are not effective against viruses as well as they may cause some other problems such as antibiotic resistance. As a result, natural antibodies such as immunoglobulin Y (IgY) from egg yolk could be a possible replacement for traditional antibiotics with less side effects. The objective of this research was production of specific IgY againt major calf diarrhea pathogens such as coronavirus, rotavirus, E. coli, and Clostridium perfringens in the egg yolk. For this purpose, this experiment was performed with two treatments (control and vaccinated group with the trivalent vaccine of coronavirus, rotavirus, and E. coli as well as Clostridium perfringens vaccine) and seven replicates in each group on W-36 Hy-Line laying hens. SDS-PAGE and Bradford methods were used for quality and quantity evaluations of IgY, respectively. Finally, the effect of freeze-drying and spray drying on IgY degradation was assessed by SDS-PAGE. The results showed that heavy and light chains of IgY were visible in the treatment group. The control group did not show any visible IgY fragments. Furthermore, the quantity of IgY secreted in egg yolk was estimated at 4.729 mg/ml. Finally, the comparison between two drying methods showed that freeze-drying did not damage IgY although spray-drying caused a substantial damage. Furthur experiments are required to validate the biological activity of the specific IgY produced in this study.

Keywords

References

1. Amro, W. A., W. Al-Qaisi, and F. Al-Razem. 2018. Production and purification of IgY antibodies from chicken egg yolk. Journal of Genetic Engineering and Biotechnology 16: 99-103.
2.Babai, R., G. Blum-Oehler, B. E. Stern, J. Hacker, and E. Z. Ron. 1997. Virulence patterns from septicemic Escherichia coli O78 strains. FEMS Microbiology Letters 149: 99-105.
3.Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry 72: 248-254.
4.Cho, Y. I., and K. J. Yoon. 2014. An overview of calf diarrhea-infectious etiology, diagnosis, and intervention. Journal of veterinary science 15: 1-17.
5.Du, E., W. Wang, L. Gan, Z. Li, S. Guo, and Y. Guo. 2016. Effects of thymol and carvacrol supplementation on intestinal integrity and immune responses of broiler chickens challenged with Clostridium perfringens. Journal of Animal Science and Biotechnology 7: 19.
6.Forte, C., L. Moscati, G. Acuti, C. Mugnai, M. P. Franciosini, S. Costarelli, S. Costarelli, G. Cobellis, and M. Trabalza-Marinucci. 2016. Effects of dietary Lactobacillus acidophilus and Bacillus subtilis on laying performance, egg quality, blood biochemistry and immune response of organic laying hens. Journal of Animal Physiology and Animal Nutrition 100: 977–987.
7.Gehad, A. E., M. M. Mashaly, H. S. Siegel, E. A. Dunnington, and P. B. Siegel. 1999. Effect of genetic selection and MHC haplotypes on lymphocyte proliferation and interleukin-2 like activity in chicken lines selected for high and low antibody production against sheep red blood cells. Veterinary Immunology and Immunopathology 68: 13–24.
8.Heegaard, P. M., Y. Fang, and G. Jungersen. 2016. Novel adjuvants and immunomodulators for veterinary vaccines. Methods in Molecular Biology 1349: 63–82.
9.Hodek H. P., P. Trefil, J. Simunek, J. Hudecek, and M. Stiborova. 2013. Optimized protocol of chicken antibody (IgY) purification providing electrophoretically homogenous preparations. International Journal of Electrochemical Science 8: 113–24.
10.Marquardt, R. R., L. Jin, J. W. Kim, L. Fang, A. A. Frohlich and S. K. Baidoo. 1999. Passive protective effect of egg-yolk antibodies against enterotoxigenic Escherichia coli K88+ infection in neonatal and early-weaned piglets. FEMS Immunology and Medical Microbiology 23: 283-288.
11.Martella, V., K. Bányai, J. Matthijnssens, C. Buonavoglia, and M. Ciarlet. 2010. Zoonotic 6 aspects of rotaviruses. Veterinary Microbiology 140: 246–255.
12.Oliver, S. P., and S. E. Murinda. 2012. Antimicrobial resistance of mastitis pathogens. Veterinary Clinics: Food Animal Practice 28: 165-185.
13.Perween, S., K. Kumar, S. K. Chandramoni, P. K. Singh, M. Kumar, and A. Dey. 2016. Effect of feeding different dietary levels of energy and protein on growth performance and immune status of Vanaraja chicken in the tropic. Veterinary world 9: 893.
14.Polson, A., M. B. von Wechmar, and M. H. van Regenmortel. 1980. Isolation of viral IgY antibodies from yolks of immunized hens. Immunological Communications 9: 475-93.
15.Rose, M. E., E. Orlans, and N. Buttress. 1974. Immunoglobulin classes in the hen's egg: their segregation in yolk and white. European journal of immunology 4: 521-523.
16.Roy, I., and M. N. Gupta. 2004. Freeze-drying of proteins: some emerging concerns. Journal of Biotechnology and Applied Biochemistry 39: 165–177.
17.Shahidi, F., and X. Q. Han. 1993. Encapsulation of food ingredients. Critical Reviews in Food Science and Nutrition. 33: 501-547.
18.Shimizu, M., R. C. Fitzsimmons, and S. Nakai. 1988. Anti-E. coli immunoglobulin Y isolated from egg yolk of immunized chickens as a potential food ingredient. Journal of Food Science 53: 1360-1366.
19.Sitnik, O., P. Jawor, W. Kopeć, T. Skiba, and T. Stefaniak. 2013. Production and characterization of egg yolk antibodies against bovine alimentary tract pathogens. Polish Journal of Veterinary Science 16: 283-91.
20.Sun, J. H., Z. Jiang, and S. Hu. 2008. Effect of four adjuvants on immune response to F4 fimbriae in chickens. Veterinary Immunology and Immunopathology 121: 107-112.
21.Sunwoo, H. H., E. N. Lee, K. Menninen, M. R. Suresh, and J. S. Sim. 2002. Growth inhibitory effect of chicken egg yolk antibody (IgY) on Escherichia coli O157:H7. Journal of Food Science 67: 1486-1494.
22.Vega, C., Bok, M., Chacana, P., Saif, L., Fernandez, F., and V. Parreño. 2011. Egg yolk IgY: protection against rotavirus induced diarrhea and modulatory effect on the systemic and mucosal antibody responses in newborn calves. Veterinary immunology and immunopathology 142: 156-169.
23.Wang, S., X. Zeng, Q. Yang, and S. Qiao. 2016. Antimicrobial peptides as potential alternatives to antibiotics in food animal industry. International journal of molecular sciences 17: 603.
Veterinary Research & Biological Products
Volume 34, Issue 1
March 2021
Pages 69-75

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History

  • Receive Date: 06 November 2019
  • Revise Date: 10 February 2020
  • Accept Date: 22 February 2020

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