Recently acquired for NetB and TpeL toxins for vaccine production to protect against avian necrotic enteritis

Document Type : Literature Review

Authors

1 Department of Anaerobic Vaccine Research and Production, Specialized Clostridia Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

2 Associate Professor Department of Anaerobic Vaccine Research and Production, Specialized Clostridia Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

3 Department of Anaerobic Vaccine Research and Production, Specialized Clostridia Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.

Abstract

Clostridium is a broad genus of rod-shaped, anaerobic, gram-positive and spore-forming bacteria. It has 203 species and is classified into five isotypes (A, B, C, D, and E) based on four major toxins, iota (iA), alpha (cpa), beta (cpb) and epsilon (etx). These bacteria are one of the most important causes of disease in animals. Various toxins of these bacteria play an important role in pathogenesis. Newly discovered toxin such as NetB and TpeL could be mentioned. These toxins are involved in a variety of diseases, including necrotic enteritis. This article presents an overview of studies on NetB and TpeL toxins. Furthermore recent advances had been provided in the production of new generation vaccines. In this article, we demonstrated that the sequences of tpeL and netB were investigated using bioinformatics software for recombinant protein production. Different regions of the gene were selected and evaluated for fusion protein synthesis. The fusion gene was synthesized by PCR and the recombinant plasmid was transformed into E. coli. Then the fusion protein was purified by NI-NTA and used for confirmation by SDS-PAGE and Western blotting. Serum neutralization test was done to evaluate of antibody in rabbits. Mutants with less cytotoxic activity were also designed by site-directed mutagenesis kits and confirmed by sequencing. The results of this study suggested that new-generation vaccines could be used as a candidate vaccine against avian necrotic enteritis.

Keywords

References

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Veterinary Research & Biological Products
Volume 34, Issue 1
March 2021
Pages 26-37

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History

  • Receive Date: 09 December 2019
  • Accept Date: 19 February 2020

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