Effects of temperature and storage time on canine hematologic parameters using the Nihon Kohden MEK-6450 hematology analyzer

Document Type : Full Research Paper

Authors

1 Graduated from Faculty of Veterinary Medicine, Urmia University

2 Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University

3 Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University.

Abstract

Delay between sampling and sample analysis can occur when blood samples are transported to reference laboratories or coincide with holidays. The aim of this study was to evaluate the stability of hematological analytes in canine blood samples using Nihon Kohden MEK-6450 hematology analyzer. Blood samples were collected from 40 clinically healthy dogs in EDTA-containing tubes and examined for 1 hour using Nihon Kohden MEK-6450 analyzer. The samples were then stored at 4 and 24 ° C and re-analyzed at 3, 6, 24 and 48 hours after collection. Significant changes in blood analytes in samples from dogs included, HCT and MCV were increased at 24 h at both temperatures (4 and 24 ° C) and was decreased in MCHC from 6 h at 4 ° C and 24 h at 24 ° C and MPV was increased at 6 h at 4 ° C, respectively. Platelet count was also decreased from 6 h at both temperatures. There were no significant changes in RBC, WBC counts and hemoglobin concentration during the study. Although most changes in hematological analytes were analytically acceptable and clinically insignificant in samples when stored at 4 and 24 ° C, however, the best way to measure hematological analytes is immediate analysis after sampling. The blood samples are preferable to be analyzed within an hour after collection.

Keywords

References

1- Abeshi, J., E. Dimco, G. Dhamo, E. Lika and E. Ozuni. 2014. Effects of Storage at Room and Refrigerator Temperatures on Dogs Blood Parameters. Albanian Journal of Agricultural Sciences 13.
2- Ameri, M., H. A. Schnaars, J. R. Sibley and D. J. Honor. 2011. Stability of hematologic analytes in monkey, rabbit, rat, and mouse blood stored at 4 degrees C in EDTA using the ADVIA 120 hematology analyzer. Vet Clin Pathol 40: 188-193.
3- Bourges-Abella, N. H., A. Geffre, P. L. Deshuillers, J. P. Braun and C. Trumel. 2014. Changes in hematology measurements in healthy and diseased dog blood stored at room temperature for 24 and 48 hours using the XT-2000iV analyzer. Vet Clin Pathol 43: 24-35.
4- Bourner, G., J. Dhaliwal and J. J. L. h. o. p. o. t. I. S. f. L. H. Sumner. 2005. Performance evaluation of the latest fully automated hematology analyzers in a large, commercial laboratory setting: a 4-way, side-by-side study. 11: 285-297.
5- Buttarello, M. J. C. C. A. 2004. Quality specification in haematology: the automated blood cell count. 346: 45-54.
6- Clark, P., T. D. Mogg, H. W. Tvedten and D. Korcal. 2002. Artifactual changes in equine blood following storage, detected using the Advia 120 hematology analyzer. Vet Clin Pathol 31: 90-94.
7- Cohle, S. D., A. Saleem and D. E. J. A. j. o. c. p. Makkaoui. 1981. Effects of storage of blood on stability of hematologic parameters. 76: 67-69.
8- Furlanello, T., S. Tasca, M. Caldin, E. Carli, C. Patron, M. Tranquillo, G. Lubas and L. Solano-Gallego. 2006. Artifactual changes in canine blood following storage, detected using the ADVIA 120 hematology analyzer. Vet Clin Pathol 35: 42-46.
9- Guder, W. J. S. j. o. c. and l. investigation. 1999. Preanalytical factors and their influence on analytical quality specifications. 59: 545-549.
10- Gulati, G. L., L. J. Hyland, W. Kocher, R. J. A. o. p. Schwarting and l. medicine. 2002. Changes in automated complete blood cell count and differential leukocyte count results induced by storage of blood at room temperature. 126: 336-342.
11- Imeri, F., R. Herklotz, L. Risch, C. Arbetsleitner, M. Zerlauth, G. M. Risch and A. R. J. C. c. a. Huber. 2008. Stability of hematological analytes depends on the hematology analyser used: a stability study with Bayer Advia 120, Beckman Coulter LH 750 and Sysmex XE 2100. 397: 68-71.
12- Lippi, G., G. L. Salvagno, G. P. Solero, M. Franchini, G. C. J. J. o. L. Guidi and C. Medicine. 2005. Stability of blood cell counts, hematologic parameters and reticulocytes indexes on the Advia A120 hematologic analyzer. 146: 333-340.
13- Medaille, C., A. Briend-Marchal and J. P. Braun. 2006. Stability of selected hematology variables in canine blood kept at room temperature in EDTA for 24 and 48 hours. Vet Clin Pathol 35: 18-23.
14- Meyer, D. and J. J. S. L. Harvey. 2004. Veterinary laboratory medicine: interpretation & diagnosis. Saunders.
15- Prins, M., M. W. van Leeuwen and E. Teske. 2009. Stability and reproducibility of ADVIA 120-measured red blood cell and platelet parameters in dogs, cats, and horses, and the use of reticulocyte haemoglobin content (CH(R)) in the diagnosis of iron deficiency. Tijdschr Diergeneeskd 134: 272-278.
16- Thrall, M. A., G. Weiser, R. Allison and T. Campbell. 2012. Veterinary hematology and clinical chemistry. John Wiley & Sons.
17- Wood, B. L., J. Andrews, S. Miller and D. E. J. A. j. o. c. p. Sabath. 1999. Refrigerated storage improves the stability of the complete blood cell count and automated differential. 112: 687-695.
Veterinary Research & Biological Products
Volume 34, Issue 1
March 2021
Pages 87-93

Files

History

  • Receive Date: 02 December 2019
  • Accept Date: 04 February 2020

Share

How to cite

Statistics