The Effect of Mycotoxin Binder Toxeat® on The Expression of INFγ and INFα Genes and Serum Levels of IL2 and IL6 in Broiler Chickens Fed with Aflatoxin

Document Type : Full Research Paper

Authors

1 Department of Microbiology, Faculty of Science, Islamic Azad University, Arak Branch, Arak, Iran

2 Animal Science Research Institute of IRAN (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

3 Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

The aim of this study was to investigate the effect of Mycotoxin Binder Toxeat® in reducing the negative effects of aflatoxin on the expression of INFγ and INFα genes and serum levels of IL2 and IL6 in broiler chicks. 300 oneday-old (Cobb 500) broiler chicks were used. They were randomly assigned to six treatments, 5 replicates and 10 birds in each unit. Experimental treatments included: Base diet (T1), Base diet+1 mg/kg of aflatoxin (T2), T2+1 g/kg Toxeat® (T3), T2+2 g/kg Toxeat® (T4), T2+3 g/kg Toxeat® (T5), T2+1 g/kg Toxeat® without inorganic section (sodium-calcium hydrated aluminosilicate hydrate) (T6). On day 42 livers were collected (3 per treatment) to evaluate changes in the expression of INFγ and INFα genes after reverse transcription of RNA extracted from liver tissue were taken. The results of this study showed that the expression of INFγ and INFα genes were different in all experimental treatments, so that the expression of INFγ gene increased significantly in treatment 3 compared to other treatments (P <0.05). Also, expression of INFα gene  in Myxotoxin Binder Toxeat® receiving treatments increased significantly compared with the control group (P <0.05). In the study of IL6 serum concentration showed a significant difference between treatments (P <0.05) but there was no significant difference in IL2 serum concentration between treatments (P> 0.05). Generally, the results of the study showed that diet supplements with Mycotoxin Binder Toxeat® could be effective in controlling the undesirable effects of Aflatoxin, it has an effect on gene expression and production and secretion of cytokines resulting in a reduction in the mortality of broiler chicks by improving the immune response

Keywords

References

1-Agboola, A.F., B.R.O. Omidiwura., O. Odu., F.T. Odupitan and E.A. Iyayi. 2015. Effect of Probiotic and Toxin Binder on Performance, Intestinal Microbiota and Gut Morphology in Broiler Chickens. Journal of Animal Science Advances 5: 1369-1379.
2-Barati, M., M. Chamani. S. N. Mousavi., S. A. Hoseini., M. Taj Abadi Ebrahimi.2017.Effect of Commercial Toxin Binder, Native Probiotic Strains, Cell Wall Yeast and Aluminosilicate in Diets Contaminated with Aflatoxin, on the Expression of GOT2, CYP450 1A5 Genes and Serum Concentrations of Liver Enzymes in Broiler Chickens. Kafkas Univ Vet Fak Derg 23: 953-960.
3-Binder, E., M. Tan., L.M. Chin., L.J. Handl and J.Richard .2007. Worldwide occurrence of mycotoxins in commodities, feeds and feed ingredients. Animal Feed Science and Technology 137: 265–282.
4-Boudergue, C., C.Burel., S. Dragacci., M.C. Favrot., J.M. Fremy., C. Massimi., P. Prigent., P. Debongnie., L. Pussemier., H. Boudra and D. Morgavi. 2009. Review of mycotoxin detoxifying agents used as feed additives: mode of action, efficacy and feed/food safety. Scientific report submitted to EFSA. Reference number of the call for proposal: CFP/EFSA/ FEEDAP/2009/01, 192pp.
5-Bovo, F., L.T. Franco., E. Kobashigawa., G.E. Rottinghaus., D.R. Ledoux and C.A.F. Oliveira.2015. Efficacy of beer fermentation residue containing Saccharomyces cerevisiae cells for ameliorating aflatoxicosis in broilers. poultry science 94: 934-942.
6-Chen, K., S.Yuan., J.Chen., X. Peng., F.Wang., H. Cui and J. Fang. 2013. Effects of sodium selenite on the decreased percentage of T cell subsets, contents of serum IL-2 and IFN-γ induced by aflatoxin B1 in broilers. Research in veterinary science. 95: 143-145.
7-Corrier, D.E. 1991. Mycotoxicosis: mechanisms of immunosuppression. Veterinary immunology and immunopathology 30: 73-87.
8-Dugyala R.R. and R.P. Sharma. 1996. The effect of aflatoxin B1 on cytokine mRNA and corresponding protein levels in peritoneal macrophages and splenic lymphocytes. International journal of immunopharmacology 18: 599-608.
9-Eaton, D.L. and J.D. Groopman. 1994. The Toxicology of aflatoxins. academic press, New York, 383-424.
10-Fani Makki, O., A.Omidi., N.Afzali., H. Sarir., M. Frouzanmehr and A. Shibak. 2014. Efficacy of Silybum Marianum Seeds in Ameliorating the Toxic Effects of Aflatoxin B1 in Broilers. Iranian Journal of Toxicology 24: 977-982.
11-He, Y., J.Fang., X.Peng., H.Cui., Z. Zuo., J.Deng., Z.Chen., W.Lai., G.Shu and L.Tang. 2014. Effects of sodium selenite on aflatoxin B1-induced decrease of ileac T cell and the mRNA contents of IL-2, IL-6, and TNF-α in broilers. Biological trace element research 159:167-173.
12-Jiang, M., X. Peng., J, Fang., H. Cui., Z.Yu and Z.Chen. 2015. Effects of aflatoxin B1 on T-cell subsets and mRNA expression of cytokines in the intestine of broilers. International journal of molecular sciences 4: 6945-6959.
13-Livak, K.J and T. D. Schmittgen. 2001. Analysis of relative gene exoression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods 25:402-408.
14-Li, Y., Q.G. Ma., L.H. Zhao., H. Wei., G.X. Duan., J.Y. Zhang and C. Ji. 2014. Effects of lipoic acid on immune function, the antioxidant defense system, and inflammation-related genes expression of broiler chickens fed aflatoxin contaminated diets. International journal of molecular sciences 15: 5649-5662.
15-Meissonnier, G.M., P. Pinton., J. Laffitte., A.M.Cossalter., Y.Y. Gong., C.P. Wild., G. Bertin., P. Galtier and I.P. Oswald. 2008. Immunotoxicity of aflatoxin B1: impairment of the cell-mediated response to vaccine antigen and modulation of cytokine expression. Toxicology and applied pharmacology 231:142-149.
16-Magan, N. 2006. Mycotoxin contamination of food in Europe: Early detection and prevention strategies. Mycopathologia.162: 245–253.
17-Merrick B.A., D.P. Phadke., S.S. Auerbach., D. Mav., S.M. Stiegelmeyer., R.R. Shah and R.R.Tice. 2013. RNA-Seq profiling reveals novel hepatic gene expression pattern in aflatoxin B1 treated rats. PloS one 8: 61768.
18-Neeff, D.V., D.R. Ledoux., G.E. Rottinghaus., A.J.Bermudez., A. Dakovic., R.A. Murarolli and C.A.F. Oliveira. 2013. In vitro and in vivo efficacy of a hydrated sodium calcium aluminosilicate to bind and reduce aflatoxin residues in tissues of broiler chicks fed aflatoxin B1. Poultry science 92: 131-137.
19-Ramakers C., J.M. Ruijter., R.H. Deprez., A.F. Moorman. 2003. Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci Lett 339: 62-66.
20-SAS Institute Inc. 2004. SAS. STAT User's Guide: Version 9. SAS Institute Inc., Cary, North Carolina.
21-Tayal, V. and B.S. Kalra. 2008. Cytokines and anti-cytokines as therapeutics—an update. European journal of pharmacology 579: 1-12.
Veterinary Research & Biological Products
Volume 33, Issue 3
September 2020
Pages 41-48

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History

  • Receive Date: 19 March 2019
  • Revise Date: 26 June 2019
  • Accept Date: 30 June 2019

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