The Role of Histological Test in Reducing The Use of Unauthorized Tissues in Meat Products Between Years of 2014 and 2017

Document Type : Full Research Paper

Authors

1 Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organisation (AREEO), Karaj, Iran

2 Food Research Institute Responsible expert, Institute of Standard and Industrial Research of Iran, Karaj, Iran

3 Department of Pathobilogy, Faculty of Veterinary Sciences, Bu-Ali Sina University, Hamadan, Iran

4 Ph.D Student of Parasitology, Urmia University, Urmia, Iran

Abstract

The prevention the using of unauthorized tissues (such as skin, bone, cartilage, soya, etc.) in the production of meat products in recent years has been very much considered. This is done to identify offending production units and increase consumer confidence in meat products. Histological method should be used to control the ingredients of meat products, along with chemical tests. The purpose of this study was to investigate the ability of histological experiments to reduce the violations of the use of unauthorized tissues in meat products (Sausages, Kielbasa, burgers, Kabab Loghmeh and nuggets). For this purpose, in the period of three years, from the second half of 2014 to the end of the first half of 2017, samples were sent to three laboratories for the detection of unauthorized tissues in the provinces of Tehran, Alborz and Esfahan were evaluated and recorded. The final results of the data recording after the end of the three-year period were evaluated by statistical software and the results were presented in the chart and picture. The results showed a significant decrease in the use of unauthorized tissues during the course of the study. By presenting the results of this research, we can be stated that histological examination as a diagnostic test has been able to play a role in preventing the use of unauthorized tissues in meat products.

Keywords


1- Abbasy-Fasarani, M., H. Hosseini, G.R. Jahed-Khaniki, M. Adibmoradi and S. Eskandari. 2013. Histological study of industrial hamburgers containing 30 and 60 percent meat for presence of unpermitted edible tissues and correlation of this factor to meat connective tissue chemical indices. Iranian Journal of Nutrition Sciences & Food Technology 7, 311-318. (In Farsi).
2- Daghighian, R., A. Javadi and S. Safavi. 2016. Histological and chemical evaluation of frauds in ground meat used for kebab in Tabriz. Journal of Food Hygiene 6, 15-26. (In Farsi).
3- Izadi, F., J. Sadeghinezhad, B. Hajimohammadi, S. Taghipourzahir, H. Fallahzadeh, M. Sheibani and A. Mirjalili. 2016. Detection of unauthorized tissues in trade frozen minced meat marketed in Yazd with histological method. Tolooebehdasht 14,423-431. (In Farsi).
4- Jahed, K.G.R. and N. Rokni. 2004. Histological detection of soya in freezing raw hamburger of Iran. Pajouhesh and Sazandegi 62,71-75. (In Farsi).
5- Jahed, K.G.R. and N. Rokni. 2007. Histological study of unpermitted tissues in heated meat products by using of masson’s trichrome stain. Pajouhesh and Sazandegi 73,96-102. (In Farsi).
6- Kamkar, A., N. Rokny, A. Rasouli and A. Shiroudi. 2004. Evaluating of hamburger quality using collagen content. Pajouhesh and Sazandegi 63,75-79. (In Farsi).
7- Latorre, R., J. Sadeghinezhad, B. Hajimohammadi, F. Izadi and M.T. Sheibani. 2015. Application of Morphological Method for Detection of Unauthorized Tissues in Processed Meat Products. Journal of Food Quality and Hazards Control 2, 71-74.
8- McNeill, S.H. 2014. Inclusion of red meat in healthful dietary patterns. Meat Science 98, 452-460.
9- Messia, M.C., T. Di Falco, G, Panfili and E. Marconi. 2008. RaPid determination of collagen in meat-based foods by microwave hydrolysis of Proteins and HPAEC-PAD analysis of 4-hydroxyProline. Meat Science 80, 401-409.
10- Prayson, B.E., J.T. McMahon and R.A. Prayson. 2007. Applying morphologic techniques to evaluate hotdogs: what is in the hotdogs we eat? Annals of Diagnostic Pathology. 12, 98-102.
11- Rao, Q., J.A. Richt and Y.H. Hsieh. 2016. Immunoassay for the Detection of Animal Central Nervous Tissue in Processed Meat and Feed Products. Journal of Agricultural and Food Chemistry 64, 3661-3668.
12- Rokni, N., M. Rezaeian, N. Nouri and F. Ebrahimpour. 2004. Detection of unauthorized tissues in some of distributed raw meat products in Tehran with histological methods. Pajouhesh and Sazandegi 17(4), 2-8. (In Farsi).
13- Sadeghinezhad, J., B. Hajimohammadi, F. Izadi, F. Yarmahmoudi and R. Latorre. 2015. Evaluation of the morphologic method for the detection of animal and herbal content in minced meat. Czech Journal of Food Science 33,564–569.
14- Sadeghinezhad, J., F. Izadi and R. Latorre. 2016. Application of histomorphological method to assess meat products. Anatomical Sciences Journal 13,73-78.
15- Suvarna, K.S., C. Layton and J.D. Bancroft. (2012). Bancroft's Theory and Practice of Histological Techniques E-Book. USA. Elsevier Health Sciences. p: 114-119.
16- Tafvizi, F. and M. Hashemzadegan. 2016. Specific identification of chicken and soybean fraud in premium burgers using multiplex- PCR method. Journal of food science and technology 53,816- 823. (In Farsi).
17- Tersteeg, M.H., P.A. Koolmees and F. Knapen. 2002. Immunohistochemical detection of brain tissue in heated meat products. Meat Science 61, 67-72.
18- Wijnker J.J., M.H. Tersteeg, B.R. Berends, J.C. Vernooij and P.A. Koolmees. 2008. Quantitative histological analysis of bovine small intestines before and after processing into natural sausage casings. Journal of Food Protection 71, 1199-1204.
19- Yaghoubifar, M., A. Shakernejad and A. Akaberi. 2009. Comparison of the Quality and safety of Sausage and Salami with Standards in Sabzevar Iran. Journal of Sabzevar University of Medical Sciences 16,114-120. (In Farsi).