Isolation and molecular identification of Mycobacterium from Rainbow fishes in Sabzevar pools (April - September 2017)

Document Type : Full Research Paper

Authors

1 Reference Laboratory for Bovine Tuberculosis, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

2 abzevar University of Medical Sciences, Faculty of Medicine, Sabzevar, Iran

3 Veterinary Aerobic Bacteria Vaccines Department, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

The members of the genus Mycobacterium, are the causative agents of mycobacteriosis, Mycobacteriosis is a chronic and progressive disease that may affect all tissues of the fish. Clinical signs include scale loss, dermal ulceration, spinal defects and etc. Mycobacteriosis is a zoonosis disease, and most people who deal with fish, are at risk. Due to the expansion of Rainbow fish breeding in the city of Sabzevar and having awareness the disease caused by Mycobacterium in fish and as a result of mortality and economic damage and also possibility of transferring this bacterium from fish to humans, it is necessary to study Mycobacterium in Sabzevar Rainbow fish. The purpose of this research is isolation and molecular identification of Mycobacterium from Rainbow fishes in Sabzevar pools. In this research 50 Rainbow fishes with clinical signs collected from seven different fish breeding pools in Sabzevar city. After autopsy and culture of the specimens in sterile conditions, in order to confirm acid-fast of the bacteria, was performed Ziel-Neelsen staining. Then, DNA extraction was performed and PCR-16s rRNA was performed to confirm mycobacterium infection. To determine identity, the results of the sequencing of the PCR product were evaluated using the BLAST program. In this research, three acidfast isolates grew, that the sequence of all three isolates belonged with 99% similar to Mycobacterium peregrinum. Given that M. peregrinum is isolated from aquatic and human respiratory tract infections, Therefore, it is necessary to warn people who deal with fish in any way.

Keywords

References

1- Akbari, Sh., N. Mosavari, K. Tadayon, and H. Rahmati-Holasoo. 2014. Isolation of Mycobacterium fortuitum from fish tanks in Alborz, Iran. Iranian journal of Microbiology. 6(4): p. 234–239.
2- Antuofermo, E., A. Pais, M. Polinas, T. Cubeddu, M. Righetti, M.A. Sanna, and M. Prearo. 2017. Mycobacteriosis caused by Mycobacterium marinum in reared mullets: first evidence from Sardinia (Italy). Journal of Fish Diseases. 40(3): p. 327-337.
3- Aranaz, A., A. J. Gibello, J. Alvarez, and A. I. Mata. 2008. Mycobacterium peregrinum infection in farmed European tench (Tinca tinca L). Veterinary Microbiology.131: p. 393-399.
4- Bragg, R. R., Hildegard, F. A. K. Huchzermeyer, and Monica A. M. Hanisch. 1990. Mycobacterium fortuitum Isolated from three species of fish in South Africa. Onderstepoort Journal of Veterinary Research. 57: p.101-102.
5- Gauthier, D.T. and M. W. Rhodes. 2009. Mycobacteriosis in fishes: A review. The Veterinary Journal. 180(1): p. 33-47.
6- Gcebe, N., A. L. Michel., and T. M. Hlokwe. 2018. Non-tuberculous Mycobacterium species causing mycobacteriosis in farmed aquatic animals of South Africa. Biology Medicine Central. 18: (32).
7- Ghazi-Saeidi, K., R. Hashemzadeh, M. Mohammadi, F. D. Fatemi-Nasab, and E. Ghaemi. 2006. Mycobacterium mainum infection in caviar fishes and fishermans in Ashorada of Golestan province in North of Iran. Journal of Gorgan University of Medicl Sciences. 8(2): p. 60-62. (In Farsi).
8- Hashish, E., A. Merwad, Sh. Elgaml, A. Amer, H. Kamal, A. Elsadek, A. Marei, and M. Sitohy. 2018. Mycobacterium marinum infection in fish and man: epidemiology, pathophysiology and management; a review. Veterinary Quarterly. 38(1): p. 35-46.
9- Hongslo, T. and E. Jansson. 2014. Occurence of different species of mycobacteria in aquarium fish from Swedish pet-shops. Bulletin of the European Association of Fish Pathologists. 34(3): p. 78-86.
10- Kamijo, F., H. Uhara, and H. Kubo. 2012. A Case of Mycobacterial Skin Disease Caused by Mycobacterium peregrinum, and a Review of Cutaneous Infection. Case Reports in Dermatology. 4(1): p. 76-79.
11- Kusar, D., U. Zajc, V. Jencic, M. Ocepek, J. Higgins, M. Zolnir-Dovc, and M. Pate. 2017. Mycobacteria in aquarium fish: results of a 3year survey indicate caution required in handling pet-shop fish. Journal of Fish Diseases. 40(6): 773-784.
12- Lescenko, P., L. Dvorska, L. Matlova, and I. Pavlik. 2003. Mycobacterial infection in aquarium fish. Veterinary Medicina. 48(3): p. 71-78.
13- Mobasher-Amini, A. 2017. Isolation, Molecular identification and DNA fingerprinting of Mycobacterium from fish culture farms offer in Karaj city. MSc thesis. Tabriz Higher Education Institude of Rab- rashidgraduation. Tabriz, Iran.
14- Mohajeri, P., L. Yazdani, A. Hashemi shahraki, A. Alvandi, S. Atashi, A. Farahani, A. Almasi, and M. Rezaei. 2016. Verification of Frequency in Speceis of Nontuberculous Mycobacteria in Kermanshah Drinking Water Supplies Using the PCR-Sequencing Method. Microbial Drug Resistance. 23(3): p. 359-364.
15- Mouton, E., L. Basson, D. Impson. 2001. Healthstatus of ornamental freshwater fishes imported to South Africa: a pilot study. Aguarium Science and Conservation. 3(4): p. 313- 319.
16- Pourahmad, F., M. Nemati, and R.H. Randolph. 2013. Comparison of three methods for detection of Mycobacterium marinum in goldfish (Carassius auratus). Aquaculture. 422-423: p. 42-46.
17- Puk, K., T. Banach, A. Wawrzyniak, and L. Adaszek. 2017. Detection of Mycobacterium marinum, Mycobacterium peregrinum, Mycobacterium fotuitum, Mycobacterium abscessus in aquarium fish. Journal of Fish Diseases. 4(1): p. 1-5.
18- Rahbar, F., A. Lamei, and H. Babazadeh. 2010. Isolation of rapid growing mycobacteria from soil and water in Iran. African Journal of Biotechnology. 24(9): p. 3618-3621.
19- Todorval, T. T., V. Kaludova, G. Tsankoval, and N. Ermenlieva. 2015. A pulmonary infection caused by Mycobacterium peregrinum- a case report. Journal of IMAB. 21(4): p. 1000-1002.
20- Van Soolingen, D. T., T. Hoogenboezem, PE. deHaas, PW. Hermans, MA. Koedam, KS. Teppema, PJ. Brennan, GS. Besra, F. Portaels, J. Top, LM. Schouls, and JD. van Embden. 1997. A novel pathogenic taxon of the Mycobacyetium tuberculosis complex, canetti: characterization of an exceptional isolate from Africa. International Journal of Sestematic Bacteriology. 47: p. 1236- 1245.
21- Whipps, C.M., S. T. Dougan, and M. L. Kent. 2007. Mycobacterium haemophilum infection of zebrafish in research facilities. Federation of European Microbiological Societies Microbiolog Letters. 270(1): p.21-26.
22- Whipps, C.M., C. Lieggi, and R. Wagner. 2012. Mycobacteriosis in Zebrafish Colonies. Institude for Laboratory Aminal Research. 53(2): p. 95–105.

Files

History

  • Receive Date: 14 November 2018
  • Revise Date: 04 May 2019
  • Accept Date: 06 May 2019

Share

How to cite

Statistics