Effect of ketamine on pentylenetrazole - induced experimental epileptiform activity in male rat

Document Type : Full Research Paper

Author

Assistant Professor, PhD Pharmacology, Basic Sciences Department, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.

Abstract

Epilepsy is one of the chronic diseases of the central nervous system. Despite the many drugs used to treat it, their effectiveness is questionable. So the purpose of this study was to investigate the stimulatory and protective effect of ketamine on experimental - epileptic activity induced by intraperitoneal injection of pentylenetetrazole in adult male rats. In this study, 15 male rats (200-250 gr) were used and randomly divided to control groups (n=5) that received normal saline and two treatment groups that received ketamine 20 mg/kg (n=5) and the second group that received ketamine 50 mg/kg (n=5) intraperitoneally. After anesthesia with ketamine-xylasein combination and animal skull surgery, the intradermal electrodes were placed on the striatum pyramidal layer of CA1 region of the hippocampus and epileptiform activity was induced by intraperitoneal injection of pentylenetetrazole and was evaluated in terms of the number of spikes per unit of time and amplitude of them by eTrace software. The results of this study showed that ketamine intraperitoneal doses of 20 and 50 mg/kg had no significant effect on the epileptic activity induced by pentylenetetrazole. Intraperitoneal injection of ketamine 20 and 50 mg/kg has no significant effect on pentylenetrazole induced seizure activity and has no stimulatory and inhibitory effects in the desired activities in that doses.

Keywords

References

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Veterinary Research & Biological Products
Volume 33, Issue 1
March 2020
Pages 101-107

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History

  • Receive Date: 06 May 2019
  • Revise Date: 06 July 2019
  • Accept Date: 06 July 2019

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