An investigation on the anthelmintic effect of Nigella sativa L. extract on the infective larva of Parascaris equorum in vitro

Document Type : Full Research Paper

Authors

1 Department of Pathobiology, School of Veterinary Medicine, Shiraz University.

2 Department of Pathobiology, School of Veterinary Medicine, Shiraz University

3 Department of Clinical Studies, School of Veterinary Medicine, Shiraz University

Abstract

In this study, the anthelmintic effects of the medicinal herb, Nigella Sativa L., was screened in vitro against the infective larvae of Parascaris equorum. Follow the collection of Parascaris worms, the egg content were depleted and hatched using sodium hypochlorite solution. The recovered larvae of the parasite were exposed to six concentrations (6.25, 12.5, 25, 50, 100 and 200 mg/mL) of the Nigella extract and then examined for the viability at 0, 10, 20, 30, 40, 50 and 60 minutes after the challenge. The results revealed that the concentration of 6.25 mg/mL had not any significant anthelmintic effects on P. equorum larvae. In contrast, the statistics indicated that all the higher concentrations of the Nigella extract remarkably reduced the viability of larva. These results were obvious during the first ten minutes of the experiment. With increase in time of exposure, the mortality effect of the extract elevated significantly. In addition, all larvae were inactivated after 40, 20 and 10 minutes after exposure at the concentrations of 50, 100 and 200 mg/ml, respectively. These results confirmed that this herbal extract possess good antiparasitic effects against P. equorum infections.

Keywords

References

1- Ademola, I.O. and J.N. Eloff. 2010. In vitro anthelmintic activity of Combretum molle (Combretaceae) against Haemonchus contortus ova and larvae. Veterinary Parasitology, 169: 198-203. 
2- Akhtar, M.S., Z. Iqbal, M.N. Khan and M. Lateef. 2000. Anthelmintic activity of medicinal plants with particular reference to their use in animals in the Indo Pakistan subcontinent. Small Ruminant Research, 38: 99-107.
3- Akhtar, M.S. and S. Riffat. 1991. Field trial of Saussurea lappa roots against nematodes and Nigella sativa seeds against cestodes in children. Journal of Pakistan Medical Association, 41: 185-187.
4- Albuquerque, U.P., P.M. Medeiros, A.L. Almeida, J.M. Monteiro and E.M.F.L. Neto, J.G. Melo and J.P. Santos. 2007. Medicinal plants of the caatinga (semi-arid) vegetation of NE Brazil. Journal of Ethnopharmacology,114: 325-354.
5- Al-Mayah, K.Sh., N.M. Al-Bashir and B.M. Al-Azzawi. 2012. In vivo efficacy of Nigella Sativa aqueous seed extract against metacestode of Echinococcus granulosus. Medical Journal of Babylon, 9(1): 140-151.
6- Al-Shaibani, I.R.M., M.S. Phulan, A. Arijo, T.A. Qureshi and A.M. Kumbher. 2008. Anthelmintic activity of Nigella sativa L, seeds on gastrointestinal nematodes of sheep. Pakistan Journal of Nematology, 26(2): 207-218.
7- Asase, A., A.A. Oteng-Yeboah, G.T. Odamtten, M.S.J. Simmonds. 2005. Ethnobotanical study of some Ghanaian anti-malarial plants. Journal of Ethnopharmacology, 99(2): 273-27.
8- Athanasiadou, S., O. Tzamaloukas, I. Kyriazakis, F. Jackson and R. Coop. 2005. Testing for direct anthelmintic effects of bioactive forages against Trichostrongylus colubriformis in grazing sheep. Veterinary Parasitology, 127: 233-243.
9- Ayaz, E., H. Yilmaz, H. Ozbek, Z. Tas and O. Orunc. 2007. The effect of Nigella sativa oil against Aspiculuris tetraptera and Hymenolepis nana in naturally infected mice. Saudi medical Journal, 28: 1654-1657.
10- Boersema, J.H., M. Eysker and J.W. Nas. 2002. Apparent resistance of Parascaris equorum to macrocyclic lactones. Veterinary Record, 150: 279-281.
11- Boyle, A.G. and R. Houston. 2006. Parasitic pneumonitis and treatment in horses; Clinicla Techniques in Equine Practice, 5: 225-232. 
12- Clayton, H.M. 1986 Ascarids recent advances. Veterinary Clinics of North America: Equine Practice, 21: 313-328.
13- Githiori, J.B., S. Athanasiadou and Thamsborg S.M. 2006. Use of plants in novel approaches for control of gastrointestinal helminths in livestock with emphasis on small ruminants. Veterinary Parasitology, 139(4): 308-320.
14- Hammond, J.A., D. Fieding and S.C. Bishop. 1997. Prospects for plant anthelmintics in tropical veterinary medicine. Veterinary Research Communications, 2: 213-228.
15- Hearn, F.P.D. and A.S. Peregrine. 2003. Identification of foals infected with Parascaris equorum apparently resistant to ivermectin. Journal of Veterinary Medicine Association, 233: 482-485.
16-Hounzangbe-Adote, M.S., V. Paolini, I. Fouraste, K. Moutairou, H. Hoste. 2005. In vitro effects of four tropical plants on three life-cycle stages of the parasitic nematode, Haemonchus contortus. Research in Veterinary Science, 78: 155-160.
17- Hördegen, P., H. Hertzberg, J. Heilmann, W. Langhans and V. Maurer. 2007. The anthelmintic efficacy of five plant products against gastrointestinal trichostrongylids in artificially infected lambs. Veterinary Parasitology, 117: 51-60.
18- Hussain, A., M.N. Khan, Z. Iqbal and M.S. Sajid. 2008. An account of the botanical anthelmintics used in traditional veterinary practices in Sahiwal district of Punjab, Pakistan. Journal of Ethnopharmacology, 119: 185-190.
19- Iqbal, Z., M.A. Munir, M. Khan, M.S. Akhtar and I. Javed. 2001. In vitro inhibitory effects of Sorghum bicolor on hatching and moulting of Haemonchus contortus eggs. International Journal of Agriculture and Biology, 3: 451-453.
20- Iqbal, Z., M. Lateef, M. Ashraf and A. Jabbar. 2004. Anthelmintic activity of Artemisia brevifolia in sheep. Journal of Ethnopharmacology, 93: 265-268.
21- Jabbar, A., M.A. Zaman, Z. Iqbal, M. Yaseen and A. Shamim. 2007. Anthelmintic activity of Chenopodium album (L.) and Caesalpinia crista (L.) against trichostrongylid nematodes of sheep. Journal of Ethnopharmacology, 114: 86-91.
22- Mahmoud, M.R., H.S. El-Abhar and S. Saleh. 2002. The effect of Nigella sativa oil against the liver damage induced by Schistosoma mansoni infection in mice. Journal of Ethnopharmacology, 79: 1-11.
23-Melo, A.C., I.F. Reis, C.M.L. Bevilaqua, L.S. Vieira, F.A.M. Echevarria and Melo, L.M. 2003. Nematódeos resistentes a anti-helmínticos em rebanhos de ovinos e caprinos do estado do Ceará, Brasil. Ciênc Rural, 33: 339-344.
24- Näreaho, A., K. Vainio and A.Oksanen. 2011. Impaired efficacy of ivermectin against Parascaris equorum, and both ivermectin and pyrantel against strongyle infections in trotter foals in Finland. Veterinary Parasitology, 182: 372–377.
25- Niezen, J., T. Waghorn, W. Charleston and G. Waghorn. 1995. Growth and gastrointestinal nematode parasitism in lambs grazing either lucerne (Medicago sativa) or sulla (Hedysarum coronarium) which contains condensed tannins. Journal of Agricultural Science, 125: 281-289.
26- Rakhshandehroo, E., M. Asadpour, A. Jafari and S.H. Malekpour. 2016. The effectiveness of Cinnamomum zeylanicum, Punica granatum flower and Capsicum annuum extracts against Parascaris equorum infective larvae. Journal of Faculty of Veterinary Medicin Istanbul University, 42(2): 132-137.
27- Rakhshandehroo, E., M. Asadpour, A. Jafari and S.H. Malekpour. 2016. The anthelmintic effects of five plant extracts on the viability of Parascaris equorum larvae. Equine Veterinary Education, 29: 219-224.
28- Rizvi, W., A. Kumar, N.H. Rizvi, R. Ahmad, K.C. Singhal and A. Khan. 2005. Antifilarial activity of Nigella sativa on Setaria cervi-an in vitro study. Oriental Pharmacology and Experimental Medicine, 5(3): 240-245.
29- Rochfort, S., A.J. Parker and F.R. Dunshea. 2008. Plant bioactives for ruminant health and productivity. Phytochemistry, 69: 299-322.
30- Shalaby, H.A. and F.M. El-Moghazy. 2013. In vitro effect of Nigella sativa oil on adult Toxocara vitulorum. Pakistan Journal of Biological Science 16: 1557-1562.
Veterinary Research & Biological Products
Volume 33, Issue 1
March 2020
Pages 33-40

Files

History

  • Receive Date: 29 January 2019
  • Revise Date: 22 March 2019
  • Accept Date: 20 April 2019

Share

How to cite

Statistics