Cross-reactive immunogenic proteins of P.multocida serotypes: candidates for improving vaccine

Document Type : Full Research Paper

Authors

1 Department of Avian Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

2 Department of Cellular Molecular Biology, Faculty of Life Sciences, Kharazmi University, Karaj, Iran

Abstract

Because P.multocida serotypes have no cross protection Several studies have been focused on identification of immunogenic molecules shared between different serotypes in the hope of production of a wide-range vaccine effective against all causative serotypes. In the present study, crude sonicated cell extracts of P. multocida serotypes A:1, A:3, and A:4 were immunoblotted against chicken hyper-immune sera in order to identify cross-reactive immunogenic proteins of them. 35 chickens aged two months randomly divided into 7 groups of five and were injected by monovalent or trivalent serotypes with time intervals of 14 days between first and second injections, and 10 days between second and third and third to fourth injections. The first inoculum was adjuvanted with complete Freund adjuvant (CFA), the second and third inocula were adjuvanted with incomplete Freund adjuvant (IFA), and the fourth one was without any adjuvant. Results showed that there are at least 5 cross-reactive immunogenic proteins in serotypes A:1, A:3, and A:4, in a way that antibodies produced by chicken in an immune response to immunization with whole cell Pasteurella suspensions are able to react with each of the 3 serotypes. These cross-reactive agents are proteins (or at least in some parts) with the molecular weight of 39, 40, 37, 53, and 30 K.Da. The 39 K.Da band was the most prominent immunoblott and introduced as candidate for molecular identification and future vaccine studies.

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Main Subjects

References

1-Ali, H. A., T. Sawada, H. Hatakeyama, N. Ohtsuki and O. Itoh.2004. Characterization of a 39kDa capsular protein of avian Pasteurella multocida using monoclonal antibodies. Veterinary Microbiology 100: 43-53
2-Ataei, S., R. Burchmore, , C. Hodgson, , A. Finucane, R. Parton, , and J.G. Coote. 2009.“Identification of immunogenic proteins associated with protection against haemorrhagic septicaemia after vaccination of calves with a live-attenuated aroA derivative of Pasteurella multocida B:2”. Research in Veterinary Science 87(2): 207-210
3-Borrathybay, E., T. Sawada, Y. Kataoka, N. Ohtsu, M. Takagi, S. Nakamura and E. Kawamoto. 2003. A 39 kDa protein mediates adhesion of avian Pasteurella multocida to chicken embryo fibroblast cells. Veterinary Microbiology 97, 229-243
4-Boyce, J. D. and B. Adler.2001. Acapsular Pasteurella multocida B:2 can stimulate protective immunity against pasteurellosis. Infection and Immunity 69:1943-1946.
5-Boyce, J. D., P. A. Cullen, V. Nguyen, I. Wilkie, and B. Adler.2006. Analysis of the Pasteurella multocida outer membrane sub-proteome and its response to the in vivo environment of the natural host. Proteomics 6:870-880.
6-Confer A.W.2009. Update on bacterial pathogenesis in BRD. Animal Health Research Review 10:145–148.
7-Dabo S.M, A.W. Confer and R.A. Quijano-Blas. 2003.Molecular and immunological characterization of Pasteurella multocida serotype A:3 OmpA: evidence of its role in P. multocida interaction with extracellular matrix molecules. Microbial Pathology. 35(4):147-57.
8-De Alwis, M.C.1993. Pasteurellosis in Production Animals: a Review. pp. 11-22. In B. E. Patten, Pasteurellosis in Production Animals. Australian Centre for International Agricultural Research (ACIAR).
9- Gong, Q., N. Qu, M. F. Niu, and C.L. Qin. 2016. Evaluation of immunogenicity and protective efficacy of recombinant ptfA of avian Pasteurella multocida. Iranian Journal of Veterinary Research, Shiraz University 17(2): 84-88
PMCID: PMC5090136
10- Greenwood, D., R.C. Slack and J. F. Peutherer.2002. Medical Microbiology, a guide to microbial infections: pathogenesis, immunity, laboratory diagnosis and control, Sixteenth ed: Churchill Livingstone. London
11- Harper, M., J.D. Boyce, I.W. Willkie and B. Adler.2003. Signature-tagged mutagenesis of Pasteurella multocida identifies mutants displaying differential virulence characteristics in mice and chickens. Infection and Immunity 71:5440-5446.
12 - Hatfaludi, T., K. Al-Hasani, J.D. Boyce and B. Adler, (2010). Outer membrane proteins of Pasteurella multocida. Veterinary Microbiology 144: 1–17.
13- Xiao, K., Q. Liu, X. Liu, Y. Hu, X. Zhao and Q. Kong.2016. Identification of the Avian Pasteurella multocida phoP Gene and Evaluation of the Effects of phoP Deletion on Virulence and Immunogenicity. International journal of Molecular Sciences 17:12-20
14- Luo, Y., Q. Zeng, J. R. Glisson, M.W. Jackwood, I.H. Cheng and C. Wang.1999. Sequence analysis of Pasteurella multocida major outer membrane protein (OmpH) and application of synthetic peptides in vaccination of chickens against homologous strain challenge. Vaccine 17: 821-831.
15 -May, B.J, Q. Zhang, L.L Li, M.L Paustian , T.S. Whittam, V. Kapur, .2001. Complete genomic sequence of Pasteurella multocida, Pm70. Proceedings of the National Academy of Sciences of United States of America 98(6):3460-5.
16- Muniandy, N., D.N. Love and T.K. Mukkur. 1998. Immunogenicity of purified lipopolysaccharide or protein-oligosaccharide conjugates of Pasteurella multocida type 6:B in mice. Comp Immunol Microbiol Infect Dis 21, 257-279.
17- OIE. 2004. HAEMORRHAGIC SEPTICAEMIA. In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 5th edition, 2004: OIE.available online at: www.oie.int/fileadmin/Home/eng/...in.../HAEMORRHAGIC_SEPTICEMIA.pdf
18- Pandher, K., A.W. Confer and G.L. Murphy.1998.Genetic and immunologic analyses of PlpE, a lipoprotein important in complement-mediated killing of Pasteurella hemolytic serotype 1. Infection and Immunity 66(12):5613-9.
19- Rimler, R. B. and K.R. Rhoades. 1989. Solubilization of membrane-associated cross-protection factor(s) of Pasteurella multocida. Avian Diseases 33: 258-263.
20- Rimler, R. B. 2001. Purification of a cross-protective antigen from Pasteurella multocida grown in vitro and in vivo. Avian Diseases 45:572-580.
21- Roier, s., J.C. Fenninger, D.R. Leitner, G.N. Rechberger, J. Reidl and S. Schild.2006.Immunogenicity of Pasteurella multocida and Mannheimia haemolyticaouter membrane vesicles. International Journal of Medical Microbiology 303(5): 247–256.
22- Singh, A.P., S. Singh, R. Ranjan, S.K. Gupta, V.P. Singh, and B. Sharma.2010.Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2. Journal of Veterinary Sciences 11(3):227-33.
23- Tabatabai, L. B. and E. S. Zehr.2004. Identification of five outer membrane-associated proteins among cross-protective factor proteins of Pasteurella multocida. Infecion and Immunity 72:1195-1198.
24- Watt, J. M., E. Swiatlo, M.M. Wade and F.R. Champlin .2003. Regulation of capsule biosynthesis in serotype A strains of Pasteurella multocida. FEMS Microbiology Letter 225: 9-14
25- Wu, J.R., J.H. Shien, H.K. Shieh, C.F. Chen and P.C. Chang 2007. Protective immunity conferred by recombinant Pasteurella multocida lipoprotein E (PlpE). Vaccine 25(21):4140-8.
Veterinary Research & Biological Products
Volume 32, Issue 3
September 2019
Pages 38-46

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History

  • Receive Date: 17 December 2017
  • Revise Date: 02 February 2019
  • Accept Date: 03 February 2019

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