Phylogenetic analysis of Iran capripoxvirus isolates during 2013-2016

Document Type : Full Research Paper

Authors

1 Agricultural Research, Education and Extention Organization (AREEO), Razi vaccine & Sera institute, Karaj, Iran.

2 Agricultural Research, Education and Extention Organization (AREEO), Razi vaccine & Sera institute, Karaj, Iran

Abstract

Capripoxvirus (CaPV) genus of Poxviridae family comprises three closely related viruses, namely sheeppox virus (SPV), goatpox virus (GPV) and lumpy skin disease virus (LSDV) causing sheeppox and goatpox and lumpy skin disease of cattle, respectively, with significant economic impact on livestock in endemic areas. Sheeppox and goatpox are endemic for many past years, and Lumpy skin disease emergently occurred in recent years in Iran. Since capripoxviruses are morphologically and antigenically identical discrimination of them relies exclusively on the use of molecular tools. The aim of this study is the molecular characterization of Iran capripoxviruses based on sequence analysis of the chemokine receptor (CKR) homologue gene. For this purpose, 16 capripoxvirus isolates consist of 10 SPV, 4 GPV and 2 LSDV isolates from different parts of Iran during 2013-2016 were used. CKR genes of isolates were amplified by PCR and cloned to pTZ57R/T vector. They were sequenced and analyzed by comparing to those of CaPVs available in the Genbank. Sequence and phylogenetic analysis showed that Iran SPV isolates with more than 99% identity were grouped on the same SPV clade retrieved from Genbank, except for Oman SPV. Iran GPV isolates with 98-99% identity were clustered with those of other countries and furthermore, with India and Bangladesh GPV isolates showed the same genetic profile. Iran LSDV isolates were 99-100% identical with other LSDs in the same clade. Conclusively, Iran SPV, GPV, and LSDV isolates are quite different based on sequence analysis of the CKR gene.

Keywords

Main Subjects

References

1- Diallo, A. and G.J. Viljoen, Genus capripoxvirus, in Poxviruses. 2007, Springer. p. 167-181.
2-Bhanuprakash, V., et al., An epidemiological study of sheep pox infection in Karnataka State, India. Revue scientifique et technique-Office international des épizooties, 2005. 24(3): p. 909.
3-3Davies, F.G., Lumpy skin disease, an African capripox virus disease of cattle. British Veterinary Journal, 1991. 147(6): p. 489-503.
4-Yune, N. and N. Abdela, Epidemiology and economic importance of sheep and goat pox: a review on past and current aspects. J Vet Sci Technol, 2017. 8(2): p. 1-5.
5- Carn, V., Control of capripoxvirus infections. Vaccine, 1993. 11(13): p. 1275-1279.
6- Perrin, A., et al., Recombinant capripoxviruses expressing proteins of bluetongue virus: evaluation of immune responses and protection in small ruminants. Vaccine, 2007. 25(37-38): p. 6774-6783.
7- Kitching, R., P. Bhat, and D. Black, The characterization of African strains of capripoxvirus. Epidemiology & Infection, 1989. 102(2): p. 335.343-
8- Babiuk, S., et al., Quantification of lumpy skin disease virus following experimental infection in cattle. Transboundary and Emerging Diseases, 2008. 55(7): p. 299-307.
9- Black, D., J. Hammond, and R. Kitching, Genomic relationship between capripoxviruses. Virus Research, 1986. 5(2-3): p. 277-292.
10- Tuppurainen, E., et al., Capripoxvirus diseases: current status and opportunities for control. Transboundary and emerging diseases, 2017. 64(3): p. 729-745.
11- Mahmoud, M. and M. Khafagi, Detection, identification, and differentiation of sheep pox virus and goat pox virus from clinical cases in Giza Governorate, Egypt. Veterinary world, 2016. 9(12): p. 1445.
12- Babiuk, S., et al., Yemen and Vietnam capripoxviruses demonstrate a distinct host preference for goats compared with sheep. Journal of General Virology, 2009. 90(1): p. 105-114.
13- Kitching, R. and W. Taylor, Clinical and antigenic relationship between isolates of sheep and goat pox viruses. Tropical animal health and production, 1985. 17 :(2) p. 64-74.
14- Shakya, S., V. Rao, and R. Chandra, Characterization of capripox virus isolated from field outbreak in goats. Indian veterinary journal, 2004. 81(3): p. 241-244.
15- Le Goff, C., et al., Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination. Journal of general virology, 2009. 90(8): p. 1967-1977.
16- Cabrera-Vera, T.M., et al., Insights into G protein structure, function, and regulation. Endocrine reviews, 2003. 24(6): p. 76.781-5
17- Afonso, C., et al., Genome of deerpox virus. Journal of virology, 2005. 79(2): p. 966-977.
18- Gershon, P.D. and D.N. Black, The nucleotide sequence around the capripoxvirus thymidine kinase gene reveals a gene shared specifically with leporipoxvirus. Journal of general virology, 1989. 70(3): p. 525-533.
19- Tulman, E., et al., The genomes of sheeppox and goatpox viruses. Journal of virology, 2002. 76(12): p. 6054-6061.
20- Hosamani, M., et al., Differentiation of sheep pox and goat poxviruses by sequence analysis and PCR-RFLP of P32 gene. Virus genes, 2004. 29(1): p. 73-80.
21- Stram, Y., et al., The use of lumpy skin disease virus genome termini for detection and phylogenetic analysis. Journal of virological methods, 2008. 151(2): p. 225-229.
22- Varshovi, H.R., et al., Molecular characterizations of Iranian sheeppox and goatpox viruses by sequence analysis of chemokine receptor homologue gene. 2009.
23- Zhou, T., et al., Phylogenetic analysis of Chinese sheeppox and goatpox virus isolates. Virology journal, 2012. 9(1): p. 25.
24- Toplak, I., et al., Complete genome sequence of lumpy skin disease virus isolate Serbia/Bujanovac/2016, Detected during an Outbreak in the Balkan Area. Genome announcements, 2017. 5(35): p. e00882-17.

Files

History

  • Receive Date: 17 July 2018
  • Revise Date: 13 August 2018
  • Accept Date: 04 September 2018

Share

How to cite

Statistics