Abattoir isolation and microbiological identification of field isolates of Pasteurella multocida collected from cattle at Tehran AND Alborz slaughterhouses

Document Type : Full Research Paper

Authors

National Research Laboratory of Pasteurella, Veterinary Aerobic Bacteria Vaccines Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran.

Abstract

This abattoir-based investigation was conducted in search for presence of Pasteurella multocida, collection of its isolates, characterization of their biochemical profiles and also classification of their capsular types in Tehran and Alborz provinces. Swabs from 89 lungs and 595 nasal passages of bovine calves at a single slaughterhouse in Karaj (Karaj city) and 5 slaughterhouses in Tehran (Shahriar, Shahr-e-Ray, Robat Karim, Kahrizak and Varamin) were cultured on specific media. Among the 339 non-haemolitic individual colonies, 89 were proved P. multocida by conventional biochemical tests. The PM-PCR assay confirmed identity of 64 isolates as Pm where 60 were shown to belong to the capsular type A and a single one to capsular type B. The remaining 3 isolates were non-typeable. We believe still a lot needs to be done at the national level to fully acknowledge the epidemiology of Pm in Iran.

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References


1- Al-Maary K.S., T.M. Dawoud, A.S. Mubarak, A.M. Hessain, H.M. Galal, S.A. Kabli and M.I. Mohamed. 2017. Molecular characterization of the capsular antigens of Pasteurella multocida isolates using multiplex PCR. Saudi journal of biological sciences 24,367-370.
2- Carter G.R. 1952. The type specific capsular antigen of Pasteurella multocida. Canadian journal of medical sciences 30,48-53.
3- Croville G., C. Foret, P. Heuillard, A. Senet, M. Delpont, M. Mouahid, M.F. Ducatez, F. Kichou and J.L. Guerin. 2018. Disclosing respiratory coinfections: a broad-range panel assay for avian respiratory pathogens on a nanofluidic PCR platform. Avian pathology : Journal of the WVPA,1-8.
4- Fereidouni S., H. Modir Rousta and F. Azin. 2006. The First Report of Avian Cholera in Miankaleh Wetland, Southeast Caspian Sea. Podoces 1,71-75.
5- Ghadimipour R., M. Ghorbanpoor, D. Gharibi, M. Mayahi and A. Jabbary. 2017. Pheno-and genotypic characteristics of Pasteurella multocida avian isolates in selected provinces of Iran. Iranian Journal of Veterinary Clinical Sciences 11.
6- Gharibi D., M. Hajikolaei, M. Ghorbanpour and S. Barzegar. 2017. Isolation, molecular characterization and antibiotic susceptibility pattern of Pasteurella multocida isolated from cattle and buffalo from Ahwaz, Iran. Archives of Razi Institute 72,91-98.
7- Jabbari A., M. Esmaelzadeh and G.R. Moazeni Jula. 2006. Polymerase chain reaction typing of Pasteurella multocida capsules isolated in Iran. Iranian journal of veterinary research 7,50-55.
8- Jamali H., M. Rezagholipour, S. Fallah, A. Dadrasnia, S. Chelliah, R.D. Velappan, K.S. Wei and S. Ismail. 2014. Prevalence, characterization and antibiotic resistance of Pasteurella multocida isolated from bovine respiratory infection. Veterinary journal (London, England : 1997) 202,381-383.
9- Khamesipour F., H. Momtaz and M.A. Mamoreh. 2014. Occurrence of virulence factors and antimicrobial resistance in Pasteurella multocida strains isolated from slaughter cattle in Iran. Frontiers in microbiology 5.
10- Kock R.A., M. Orynbayev, S. Robinson, S. Zuther, N.J. Singh, W. Beauvais, E.R. Morgan, A. Kerimbayev, S. Khomenko, H.M. Martineau, R. Rystaeva, Z. Omarova, S. Wolfs, F. Hawotte, J. Radoux and E.J. Milner-Gulland. 2018. Saigas on the brink: Multidisciplinary analysis of the factors influencing mass mortality events. Science advances 4,eaao2314.
11- Sahragard I., Y. Tahamtan, M. Valadan, M. Hyati, F. Moazeni and Z. Shirazi. 2012. Development of rapid PCR method for simultaneous identification of species, specific capsular type, and toxigenicity of Pasteurella sp. isolates. Comparative clinical pathology 21,1333-1336.
12- Sellyei B., A. Thuma, D. Volokhov and Z. Varga. 2017. Comparative Analysis of Pasteurella multocida Isolates from Acute and Chronic Fowl Cholera Cases in Hungary During the Period 2005 Through 2010. Avian diseases 61,457-465.
13- Shayegh J., M. Parvizi and M. Hejazi. 2010. Diversity of caprine and ovine Pasteurella multocida isolates based on 16S rRNA gene sequencing. Iranian journal of veterinary research 11,373-378.
14- Shayegh J., J. Sharaf, P. Mikaili and H. Namvar. 2009. Pheno-and genotyping of Pasteurella multocida isolated from goat in Iran. African Journal of Biotechnology 8.
15- Shirzad-Aski H. and M. Tabatabaei. 2016. Molecular characterization of Pasteurella multocida isolates obtained from poultry, ruminant, cats and dogs using RAPD and REP-PCR analysis. Molecular biology research communications 5,123-132.
16- Shirzad Aski H. and M. Tabatabaei. 2016. Occurrence of virulence-associated genes in Pasteurella multocida isolates obtained from different hosts. Microbial pathogenesis 96,52-57.
17- Sotoodehnia A., J. Vand Yousefi and I. Aarabi. 1986. Isolation and typing of Pasteurella multocida poultry isolates from Iran. Arch Razi Inst 36,85-86.
18- Sprague L.D. and K. Tadayon. 2017. Genome Sequence of Pasteurella multocida Strain Razi_Pm0001. Genome announcements 5.
19- Tabatabaei M., G. Moazzeni Jula, A. Jabbari and M. Esmailzadeh. 2007. Pathogenicity and immunogenicity of native and mutant strains of Pasteurella multocida, the causative agents of haemorrhagic septicaemia. Iranian journal of veterinary research 8,40-44.
20- Tahamtan Y. and H. Mirghafari. 2016. Classification, capsular PCR typing and genetic diversity of Pasteurella multocida isolated from sheep and goats in Iran using Bam HI, Hind III restriction endonuclease enzymes by PCR-RFLP. Tropical biomedicine 33,535-542.
21- Tahamtan Y. and I. Sahragard. 2016. Characterization, ribotyping and capsular pcr typing of Pasteurella multocida isolated from iranian sheep and goats.
22- Tavasoli A., A. Sotoodeh, I. Aarabi and J. Vand Yosefi. 1984. A case report of fowl cholera disease in north of Iran. Arch Inst Razi 34 & 35,39-41.
23- Townsend K.M., J.D. Boyce, J.Y. Chung, A.J. Frost and B. Adler. 2001. Genetic organization of Pasteurella multocida cap Loci and development of a multiplex capsular PCR typing system. Journal of clinical microbiology 39,924-929.
24- Valadan M., A. Jabbari, M. Niroumand, Y. Tahamtan and H. Bani. 2014. Isolation and identification of Pasteurella multocida from sheep & goat in Iran. Archives of Razi Institute 69,47-55.
25- Zarlasht F. and M. Khan. 2018. A Case of Recurrent Pasteurella Bacteremia in an Immunocompetent Patient with No Animal Bite. The American journal of case reports 19,95-98.

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History

  • Receive Date: 17 April 2018
  • Accept Date: 17 April 2018

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