Evaluation of Natural killer cell activity after coinfection of chickens with Infectious bursal disease and Avian influenza virus H9N2

Document Type : Full Research Paper

Authors

1 Avian Diseases Department, Razi Vaccine and Serum Research Institute,Agricultural Research, Education and Extension Organization(AREEO), Karaj, Iran.

2 Avian Health and Disease department, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran.

3 Immunology Department, Faculty of Medicine, Jondi Shapour University, Ahvaz, Iran.

4 Microbiolog Departmenty , Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran.

Abstract

Avian influenza virus subtype H9N2 is an endemic viral disease in middle east. On the otherhand Infectious bursal disease virus is a widespread infection in chicken which causes vast destruction of immune response to various diseases. Few studies on effect of the virus on innate immunity has been done. In this research we assesed NK cell activity after coinfection of chickens with IBD virus and Avian influenza virus H9N2. Thirthy two chickens were divided in four groups randomly. Group 1and 2 were inoculated with 103 CID50 IBD virus at one day old intra bursaly.at thirthy days old chickens in groups 1 and 3 were inoculated with 106 EID50 oculonasaly. Seven days after influenza virus infection chickens were euthanized and their spleens used for NK cell activity evaluation by Cytotoxicity Detection assay Kit plus LDH, Roche. The most activity was belonged to influenza infected groups but the differences was not Meaningful. So we can concluded that IBD and AI infection has no effect on NK activity.

Keywords

Main Subjects

References

1. Allan, W.H., R.J.T. Faragher, and G.A. Cullen .1972. Immunosuppression by infectious bursal agent in chickens immunized against Newcastle disease. Veterinary research, 90: 511-513.
2. Camacho, L., K.A. Schat, J.r. Brooks and D.I. Bounous .2003. Early cell-mediated immune responses to Marek s disease virus in two chicken lines with defined major histocompatibility complex antigens. Veterinary Immunology and Immunopathology. 95:145-153.
3. Cosgrow, A.S. 1962. An apparently new disease of chicken, avian nephrosis. Avian Diseases, 6: 385-389.
4. Corley, M. and J.Giambrone .2002. Immunosuppression in Specific-Pathogen-Free Broilers Administered Infectious Bursal Disease Virus Vaccines by in ovo Route. Avian Diseases, 46(4): 810-815.
5. Fujii, S., H. Hamada, K. Fujimoto, T. Shimomura, and M. Kawakita.1999. Activated Dendritic Cells From Bone Marrow Cells of Mice Receiving Cytokine-Expressing Tumor Cells Are Associated With the Enhanced Survival of Mice Bearing Syngeneic Tumors. Blood, 93(12): 4328-4335.
6. Gobel, T.W., B. Kaspers , and L. Stangassinger .2001. NK and T cells constitute two major, functionally distinct intestinal epithelial lymphocyte subsets in the chicken. International Immunology, 13(6): 757-762.
7. Hagenaars, T. J., E. A.J. Fischer, C. A. Jansen, J.M.J. Rebel, D. Spekreijse, L. Vervelde, J.A. Backer, M.C.M. de Jong, A.P. Koets. 2016. Modeling the Innate Immune Response against Avian Influenza Virus in Chicken. PLOS ONE. Journal. pone.1-17.
8. Kim, I.J., K. Karaca, T.L. Pertile, S.A. Erickson, and J.M. Sharma.1998. Enhanced expression of cytokine genes in spleen macrophages during acute infection with infectious bursal disease virus in chickens. Veterinary Immunology and Immunopathology, 61: 331-341.
9. Kim, I.J., SK.You, H. Kim, H.Y. Yeh, and J.M. Sharma. 2000. Characteristics of bursal T lymphocytes induced by infectious bursal disease virus. Journal of Virology,74:8884–8892.
10. Lam, K.M. 1991. Infectious bursal disease virus type1-induced suppression of chicken lymphocyte response to mitogen. Avian Pathology, 20(2): 205-212
11. Neulen, M.L.,B.C. Viertlboeck , C. Straub, T. W. Göbel.2015. Identification of novel chicken CD4+ CD3− blood population with NK cell like features. Developmental and Comparative Immunology, 49(1):72-78.
12. Otim, M.O. and M. Bisgaard .2005. Aflatoxicosis, infectious bursal disease and immune response to Newcastle disease vaccination in rural chickens. Avian Pathology, 34(4): 319-323
13. Reed, L.J. and Muench, H. (1938). A simple method of estimating fifty percent endpoint. American Journal of Hygince, 27: 493-497.
14. Rehman, Z.U., C. Meng , S. Umar, M. Munir and C. Ding.2016. Interaction of infectious bursal disease virus with the immune system of poultry. World's Poultry Science Journal, 72: 805-820
15. Seo, S.H. and R.G.Webster .2001. Cross-reactive, cell-mediated immunity and protection of chickens from lethal H5N1 influenza virus infection in Hong Kong poultry markets. Journal of Virology,75: 2516-2525.
16. Sharma, J.M. And B.D. Coulson.1979. Presence of natural killer cells in specific-pathogen-free chickens. Journal of national cancer institute, 63: 527-53.
17. Sharma, J.M. And L. Lee. 1983. Effects of infectious bursal disease on chicken natural killer cell activity and mitogenic response of chicken lymphoid cells: role of adherent cells in cellular immune suppression. Infection and Immunology, 42(2) : 747-754
18. Shoshtari A.H., S.A. Pourbakhsh, , H.A. Dadras, M.A. Bahmaninejad and R. Toroghi. 2004.Pathogenicity Study and Restriction Enzyme Profile of a Recently Isolated Infectious Bursal Disease Virus in Iran. Arch. Razi Ins. 58: 9-18
19. Thompson, G., H. Mohammed, B. Bauman, and S. Naqui .1997. Systemic and local antibody response to infectious bronchitis virus in chickens inoculated with infectious bursal disease virus and control chickens. Avian Disease, 41: 519-527.
20. Toro, H., J.C. Effler, F.J. Hoerr, and F.W. van Ginkel.2009. Pathogenicity of Infectious Bursal Disease Virus Variant AL2 in Young Chickens. Avian Diseases, 53(1):78-82
21. Thayer, S.G. and B.W. Charles.2008. Serological Procedures. pp:222-229. In: Zavala, L.D.; Swayne, DE. Glison, J.R. Pearson, J.E. Reed, W.M. Jackwood, M.W. And Woolcock, PR.(eds), A Laboratory Manual For the Isolation, Identification and Characterization of Avian Pathogens. 5th edition, Omni press, Madison, USA.
22. Utke, K., S. Bergmann, N. Lorenzen, and U. Fischer.2007. Cell mediated cytotoxicity in rainbow trout, infected with viral haemorrhagic septicaemia virus. Fish and Shellfish Immunology, 22: 182-196.
23. WHO.2002. WHO manual on animal influenza diagnosis and surveillance. http://www.who.int/csr/resources/publications/influenza/whocdscsrncs20025rev.pdf/Accessed 1/21/ 2018
24. Williams, H.A., and T.F. Davison .2005. Enhanced immunopathology induced by very virulent infectious bursal disease virus. Avian Pathology, 34 (1): 4 – 14.
25. Yeh, H.Y., M.K. Njenga, And J.M. Sharma .2002. Role of intrabursal T cells in infectious bursal disease virus infection. Archive of Virology,147(2): 285-304.

Files

History

  • Receive Date: 22 January 2018
  • Revise Date: 11 March 2018
  • Accept Date: 07 April 2018

Share

How to cite

Statistics