Investigation of the expressions of the C-type Lysozyme gene in head kidney, liver, pancreas and spleen tissues in persian breeding sturgeon (Acipenser persicus Borodin, 1897) by using real time RT- PCR assays

Document Type : Full Research Paper

Authors

1 Department of Fisheries and Forestry, Faculty of natural resource, Gonbad Kavous University, Golestan, Iran.

2 Assistant professor, Department of Fisheries and Forestry, Faculty of natural resource, Gonbad Kavous University, Golestan,Iran.

3 Food Hygiene and Public Health Department, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman.

4 Associate professor, Department of Fisheries and Forestry, Faculty of natural resource, Gonbad Kavous University, Golestan, Iran.

Abstract

Lysozymes are important proteins of the innate immune system for the defense against bacterial infection, especially gram-positive bacteria in fishes. According to the ability to transfer this gene from breeders to the next generation, as well as recent advantage-ground in molecular genetics on applying novel methods in the field of mRNA analysis using real time RT-PCR to measure the expression of the different genes, the present study was conducted to evaluate the expression of the C- type lysozyme gene in tissues of liver, head of kidney, pancreas and spleen in breeding of Acipenser persicus. For this purpose, tissue sampling was done after artificial breeding from 16 fish (8 males and 8 females). The tissues were transported to the laboratory in liquid nitrogen. Total RNA was isolated and cDNA synthesis was performed from resected tissues. The mRNA expression of the C-type Lysozyme gene in different tissues were detected by real time quantitative PCR with β-actin gene as an internal control. Expression of the c-type lysozyme gene was detected in all tested tissues. The maximum copy numbers of the mRNA expression of the C-type Lysozyme gene were detected in spleen tissue and the lowest expression of this enzyme was determined in the pancreas tissue (p < 0.01). Overall, considering the role of different factors such as sex, age, size, season, water temperature, pH, toxicants, infections and type of stressors in the lysozyme activity or level, which may have eventually caused the direct activation of transcription of lysozyme gene, as well as considering the importance of breeding of sturgeons, we could claim that this gene can well be used as a reference gene for the prognosis and screening of immune deficiency and control of their environment in A. persicus.

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References

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Veterinary Research & Biological Products
Volume 31, Issue 2
May 2018
Pages 161-170

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History

  • Receive Date: 07 December 2017
  • Revise Date: 25 February 2018
  • Accept Date: 03 March 2018

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