Use of Razi variety of Toxoplasma gondii in serological diagnosis of Toxoplasma infection in compare with molecular test

Document Type : Full Research Paper

Authors

1 slamic Azad University Shiraz Branch, Department of Biochemistry

2 Research, Education and Extention Organization (AREEO), Razi Vaccine & Serum Research Institute, Shiraz, Iran.

Abstract

Toxoplasmosis is the most common parasitic zoonosis world-wide, which caused by the protozoan parasite, Toxoplasma gondii. Toxoplasmosis is the main cause of abortion in sheep. There are different ways for the diagnosis of toxoplasma infection such as cell culture, serological and molecular tests. The aim of this study was the evaluation of variety of
T. gondii which produced in Shiraz, Razi Institute for the diagnosis of toxoplasmosis by direct agglutination test in compare with ELISA and molecular test. Blood samples were collected from 184 ewes with a history of abortion in north of Fars province, Iran. Samples were investigated with molecular and serological (consist of ELISA and direct agglutination test) assays for detection of toxoplasmosis. Results showed that the rate of positive sera by use of commercial kit and agglutination test were 21% and 24% respectively. The McNamara analysis showed no significant difference between agglutination and ELISA tests and the results of Kappa analysis showed a very good agreement between of these two serological assays. Nevertheless, all samples were negative by molecular assay. The direct agglutination test was showed to be an appropriate, simple, fast and economical test for toxoplasmosis diagnosis in sheep. In conclusion, the results of present study confirmed despite the attenuation of virulence of Razi variety of Toxoplasma gondii it could be suitable for serological diagnosis of toxoplasmosis.

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References

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Veterinary Research & Biological Products
Volume 31, Issue 2
May 2018
Pages 86-92

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History

  • Receive Date: 30 May 2017
  • Revise Date: 06 January 2018
  • Accept Date: 15 January 2018

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