Effects of Dietary Rose Extract on Qualititative Parameters of Frozen-Thawed Rooster Sperm

Document Type : Full Research Paper

Authors

1 M.Sc. Department of Animal Science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.

2 Assistant professor, Department of Animal Science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.

Abstract

The objective of the present study was to investigate the effects of rose (Rosa damascena) extract on the quality of frozen-thawed rooster spermatozoa. Sixteen roosters divided into four aliquots and received a diet containing 0, 50, 100, 150 mg/kg of rose extract. On the 0, 14, 28, 42, 56 and 70th day of experiment, semen fluid were collected and sperm motility characteristics including total motility, progressive motility, linearity (LIN), straight line velocity (VSL), amplitude of lateral head displacement (ALH), curvilinear velocity (VCL), average path velocity (VAP), and sperm viability, acrosome integrity and lipid peroxidation were evaluated after cooling-thawing procedure. The treatments with 100 and 150 mg of rose extract significantly improved post-thaw total motility, ALH, VSL, and sperm viability, and reduced lipid membrane peroxidation (P<0.05). The results showed that total motility, progressive motility, sperm viability, ALH, and lipid membrane peroxidation is affected by interaction between experimental diets × time, and improved (P<0.05). However, on the 70th day of experiment, highest level of rose extract significantly reduced progressive motility (P<0.05). In conclusion, 100 or 150 mg daily consumption of rose extract in the diet, as a natural antioxidant, can improve rooster sperm quality after frozen-thawed and reduce the adverse effects of freezing process during cryopreservation on the sperm.

Keywords


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