Evaluation attenuated variety of Neospora caninum as experimental live vaccine in Mice

Document Type : Full Research Paper

Authors

1 MSC Microbiology, Islamic Azad University, Branch Shiraz, Iran.

2 Agriculture Research, Education and Extention Organization (AREEO), Razi Vaccine and Serum Research Institute, Shiraz, Iran.

Abstract

Neospora caninum is one of the most important agent cause abortion in cattle all around the world which can lead in economic loss in cattle herds. Currently, most attention is focused into the live vaccine . So far there is no effective vaccine against neosporosis since the aim of this study was to evaluate the attenuated variety of N. caninum as a potential live vaccine. Sixteen female mice BALB/c were divided randomly into conrtrol and attenuated groups. Twenty-one days after immunization, sera of all groups were taken and subjected for ELISA and gamma interferon (IFN-γ) tests. All mice were challenged by the NC-1 strain of N. caninum. One month after challenge tissue samples were collected for PCR. The results of ELISA and IFN-γ tests indicated that immune response generated in the vaccinated group. The results showed that the attenuated variety of N. caninum stimulated both humeral and cellular immune responses. The results indicated that investigations carried out on non-pathogenic strains and attenuated varieties of Neospora caninum to provide live and protective vaccine is promising.

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References


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History

  • Receive Date: 15 August 2017
  • Revise Date: 30 October 2017
  • Accept Date: 30 October 2017

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